公开(公告)号：SG11201706342PA

公开(公告)日：2017-09-28

申请号：SG11201706342P

申请日：2016-01-27

Applicant: ACADEMIA SINICA

Inventor： WU HAN-CHUNG ,  TANG CHUNG-TAO

IPC: A61K39/12 ,  A61P31/14 ,  A61P37/04 ,  C07K14/08 ,  C07K14/18 ,  C07K16/10 ,  G01N33/53

Abstract: Isolated mutant dengue virus E protein variants are disclosed. The variant comprises an amino acid sequence that is at least 80% identical to SEQ ID NO: 1 and has one or more amino acid residue substitutions at position corresponding to Asn8 (N8), Arg9 (R9), Val12 (V 12) and/or Glu13 (E13). The variant may comprise an amino acid sequence that is at least 90% identical to the SEQ ID NO: 1 and lack an infection-enhancing antibody-binding motif comprising the amino acid sequence of SEQ ID NO: 28 at domain I. An isolated nucleic acid sequence encoding the variant, a plasmid expressing the variant, a plasmid expressing a virus-like particle comprising the variant, a DNA vaccine, and a method of detecting the presence of a dengue virus in a biological sample are also disclosed.