公开(公告)号：WO2013003624A3

公开(公告)日：2013-03-14

申请号：PCT/US2012044701

申请日：2012-06-28

Applicant: ACADEMIA SINICA ,  CHANG YING-CHIH ,  WU HAN-CHUNG ,  TSENG PO-YUAN

Inventor： CHANG YING-CHIH ,  WU HAN-CHUNG ,  TSENG PO-YUAN

IPC: G01N33/53

CPC classification number: G01N33/54386 ,  C07K16/30 ,  C07K17/14 ,  G01N1/405 ,  G01N33/54393 ,  G01N33/57492

Abstract: This invention relates to a surface coating for capture circulating rare cells, comprising a nonfouling composition to prevent the binding of non-specific cells and adsorption of serum components; a bioactive composition for binding the biological substance, such as circulating tumor cells; with or without a linker composition that binds the nonfouling and bioactive compositions. The invention also provide a surface coating for capture and purification of a biological substance, comprising a releasable composition to release the non-specific cells and other serum components; a bioactive composition for binding the biological substance, such as circulating tumor cells; with or without a linker composition that binds the releasable and bioactive compositions. The present invention also discloses a novel microfluidic chip, with specific patterned microstructures to create a flow disturbance and increase the capture rate of the biological substance.

Abstract translation: 本发明涉及用于捕获循环稀有细胞的表面涂层，其包含防止非特异性细胞结合的非结垢组合物和血清成分的吸附; 用于结合生物物质的生物活性组合物，例如循环肿瘤细胞; 具有或不具有结合非结垢和生物活性组合物的接头组合物。 本发明还提供用于捕获和纯化生物物质的表面包衣，其包含可释放组合物以释放非特异性细胞和其它血清成分; 用于结合生物物质的生物活性组合物，例如循环肿瘤细胞; 具有或不具有结合可释放和生物活性组合物的接头组合物。 本发明还公开了一种具有特定图案化微结构的新型微流体芯片，以产生流动扰动并增加生物物质的捕获率。

公开(公告)号：EP2726870A4

公开(公告)日：2015-03-11

申请号：EP12805303

申请日：2012-06-28

Applicant: ACADEMIA SINICA

Inventor： CHANG YING-CHIH ,  WU HAN-CHUNG ,  TSENG PO-YUAN

IPC: G01N33/53 ,  C07K17/14

CPC classification number: G01N33/54386 ,  C07K16/30 ,  C07K17/14 ,  G01N1/405 ,  G01N33/54393 ,  G01N33/57492

Abstract: This invention relates to a surface coating for capture circulating rare cells, comprising a nonfouling composition to prevent the binding of non-specific cells and adsorption of serum components; a bioactive composition for binding the biological substance, such as circulating tumor cells; with or without a linker composition that binds the nonfouling and bioactive compositions. The invention also provide a surface coating for capture and purification of a biological substance, comprising a releasable composition to release the non-specific cells and other serum components; a bioactive composition for binding the biological substance, such as circulating tumor cells; with or without a linker composition that binds the releasable and bioactive compositions. The present invention also discloses a novel microfluidic chip, with specific patterned microstructures to create a flow disturbance and increase the capture rate of the biological substance.

Abstract translation: 本发明涉及一种用于捕获循环稀有细胞，其包含不结垢组合物，以防止非特异性细胞和血清成分的吸附的结合的表面涂层; 用于结合的生物物质，颜色的生物活性组合物：如循环肿瘤细胞; 具有或不具有左组合物做了结合不结垢和生物活性的组合物。 因此，本发明提供用于生物物质的捕获和纯化的表面涂层，其包括可释放的组合物，以释放非特异性细胞和其它血清组分; 用于结合的生物物质，颜色的生物活性组合物：如循环肿瘤细胞; 具有或不具有左组合物确实结合释放的生物活性和组合物。 因此，本发明光盘松一新颖微流体芯片，与特定的微结构图案化以产生一个流动扰动并增加生物物质的捕获率。