公开(公告)号：JP2014144958A

公开(公告)日：2014-08-14

申请号：JP2014035431

申请日：2014-02-26

Applicant: Academia Sinica ,  アカデミア シニカAcademia Sinica

Inventor： WONG CHI-HUEY ,  WU CHUNG-YI ,  YU ALICE ,  YU JOHN

IPC: A61K39/00 ,  A61K39/385 ,  A61K39/39 ,  A61P35/00

CPC classification number: A61K39/0011 ,  A61K2039/55511 ,  A61K2039/6037 ,  A61K2039/6081 ,  A61K2039/627 ,  C12N15/1137 ,  C12N2310/14

Abstract: PROBLEM TO BE SOLVED: To provide: an immunogenic composition capable of high levels of immune responses immune-targeting glycolipid antigen Globo H, which is a tumor associated sugar chain; a cancer vaccine; and a method of treating cancer.SOLUTION: The invention relates to an immunogenic composition comprising: (a) a sugar chain such as Globo H or an immunogenic fragment thereof conjugated with a carrier protein comprising diphtheria toxin cross-reacting material 197 (DT-CRM 197) through a linker such as p-nitrophenol; and (b) an adjuvant with the following structure comprising a glycolipid capable of binding CD1d on a dendritic cell such as an α-galactosyl ceramide derivative.

Abstract translation: 要解决的问题：提供：能够高水平免疫应答的免疫原性组合物免疫靶向糖脂抗原Globo H，其是肿瘤相关糖链; 癌症疫苗; 和一种治疗癌症的方法。解决方案：本发明涉及免疫原性组合物，其包含：（a）糖链，例如Globo H或其与包含白喉毒素交叉反应材料197（DT-CRM）的载体蛋白缀合的免疫原性片段 197）通过接头如对硝基苯酚; 和（b）具有以下结构的佐剂，其包含能够结合树突状细胞如α-半乳糖基神经酰胺衍生物上的CD1d的糖脂。

公开(公告)号：WO2010005735A3

公开(公告)日：2010-03-18

申请号：PCT/US2009047537

申请日：2009-06-16

Applicant: ACADEMIA SINICA ,  YU ALICE L ,  YU JOHN ,  WONG CHI-HUEY

Inventor： YU ALICE L ,  YU JOHN ,  WONG CHI-HUEY

IPC: A61K31/716 ,  A61K31/164 ,  A61K31/7008 ,  A61P35/00

CPC classification number: A61K39/0011 ,  A61K2039/55511 ,  A61K2039/6037 ,  A61K2039/6081 ,  A61K2039/627 ,  C12N15/1137 ,  C12N2310/14

Abstract: An immune composition containing Globo H or its fragment (e.g., SSEA3) and an adjuvant, e.g., a -GalCer, for eliciting immune responses against Globo H or its fragment and uses thereof in cancer treatment. Also disclosed is a method of treating cancer by inhibiting the activity of one of FUT1 and FUT2, both of which involve in Globo H biosynthesis.

Abstract translation: 包含Globo H或其片段（例如SSEA3）和佐剂（例如，-GalCer）的免疫组合物，用于引发针对Globo H或其片段的免疫应答及其在癌症治疗中的用途。 还公开了通过抑制FUT1和FUT2之一的活性来治疗癌症的方法，两者都涉及Globo H生物合成。

公开(公告)号：WO2006044616A3

公开(公告)日：2006-08-24

申请号：PCT/US2005036961

申请日：2005-10-14

Applicant: ACADEMIA SINICA ,  WONG CHI-HUEY ,  HSU HSIEN-YEH ,  HUA KUO-FENG ,  LIN CHUN-HUNG ,  HSU JASON ,  CHEN SHUI-TEIN ,  LIN KUO-I ,  YANG WEN-BIN ,  YU JOHN ,  YU ALICE

Inventor： WONG CHI-HUEY ,  HSU HSIEN-YEH ,  HUA KUO-FENG ,  LIN CHUN-HUNG ,  HSU JASON ,  CHEN SHUI-TEIN ,  LIN KUO-I ,  YANG WEN-BIN ,  YU JOHN ,  YU ALICE

IPC: A01N65/00

CPC classification number: A61K36/074

Abstract: A fucose-containing glycoprotein fraction obtainable from an extract of Ganoderma Lucidum Reishi, compositions comprising the fuconse-containing glycoprotein fraction, a method to mediate immunomodulating events associated with IL-1 gene expression, a method to stimulate the expression of an inflammatory cytokine, a method to modulate protein kinase pathways associated with inflammatory cytokine Interleukin 1, a method to induce a TLR mediated event; a method to modulate differentiation of a mononuclear cell, a method to enhance cytotoxicity of an NK cell against an NK-sensitive tumor cell, a method to activate the expression of cytokines, a method to induce Blimp-1 expression in a mouse splenic B cell or human B cell, human mature splenocytes or dendritic cell, a method to inhibit LPS induced nitric oxide production in macrophages, a method to activate spleen cells proliferation, and a method to modify the proteome of a spleen cell.

Abstract translation: 从灵芝提取物获得的含岩藻糖的糖蛋白部分，包含含有蛋白质的糖蛋白部分的组合物，介导与IL-1基因表达相关的免疫调节事件的方法，刺激炎性细胞因子的表达的方法 调节与炎症细胞因子相关的蛋白激酶途径白细胞介素1的方法，诱导TLR介导的事件的方法; 调节单核细胞分化的方法，增强NK细胞对NK敏感性肿瘤细胞的细胞毒性的方法，激活细胞因子表达的方法，在小鼠脾B细胞中诱导Blimp-1表达的方法 或人B细胞，人成熟脾细胞或树突状细胞，抑制巨噬细胞中LPS诱导的一氧化氮产生的方法，活化脾细胞增殖的方法以及修饰脾细胞的蛋白质组的方法。

公开(公告)号：AU2014388315A1

公开(公告)日：2016-11-17

申请号：AU2014388315

申请日：2014-10-28

Applicant: ACADEMIA SINICA ,  CHANG GUNG MEMORIAL HOSPITAL ,  DCB USA LLC ,  DEV CT BIOTECHNOLOGY

Inventor： YU ALICE L ,  YU JOHN ,  WANG YA-HUI ,  HSU CHUAN-LUNG ,  CHEN YI-CHANG ,  LOK YING-YUNG

IPC: G01N33/574

Abstract: An antibody against transmembrane coiled-coil domains protein 3 (TMCC3), or a binding fragment thereof, includes the CDR sequences set forth in the specification. A method for treating cancer includes administering to a subject in need thereof an antibody against a transmembrane coiled-coil domains protein 3 (TMCC3). The antibody binds to an epitope in an intercoil domain of TMCC3. A method of diagnosing or assessing a cancer condition includes assessing a level of expression or activity of a transmembrane coiled- coil domains protein 3 (TMCC3) in a sample, wherein an increase in the level of expression of activity of TMCC3 as compared to a standard indicates the presence of cancer stem cells in the sample.

公开(公告)号：AU2014201215B2

公开(公告)日：2016-05-05

申请号：AU2014201215

申请日：2014-03-05

Applicant: ACADEMIA SINICA

Inventor： WONG CHI-HUEY ,  WU CHUNG-YI ,  YU ALICE ,  YU JOHN

IPC: A61K39/00 ,  A61K39/385

Abstract: The present invention provides for an immunogenic composition comprising (a) a glycan, such as Globo H, stage-specific embryonic antigen-3 (SSEA-3) or SSEA-4, conjugated to a carrier protein through a linker; (b) an adjuvant comprising a glycolipid, such as a-galactosyl-ceramide adjuvant, wherein the composition induces an immune response, such as a higher relative level of IgG isotype antibodies compared to IgM isotype antibodies, or neutralising antibodies against at least one antigen selected from the group consisting of Globo H, Gb4, SSEA-3, and SSEA-4. Uses of the immunogenic composition for treating or inhibiting tumour growth are also provided.

公开(公告)号：CA2728341C

公开(公告)日：2019-07-02

申请号：CA2728341

申请日：2009-08-06

Applicant: ACADEMIA SINICA

Inventor： WONG CHI-HUEY ,  WU CHUNG-YI ,  YU ALICE ,  YU JOHN

IPC: A61K39/00 ,  A61K39/385

Abstract: Immunogenic compositions, cancer vaccines and methods for treating cancer are provided. Compositions compris-ing: (a) a glycan such as Globo H or an immunogenic fragment thereof, wherein the glycan is conjugated with a carrier protein by a linker such as para-nitrophenyl; and (b) an adjuvant comprising glycolipid capable of binding CDId on a dendritic cell, such as an a-galactosyl-ceramide derivative, wherein the immunogenic composition induces an immune response that induces a higher relative level of IgG isotype antibodies as compared to IgM isotype antibodies, are provided. Immunogenic compositions compris-ing the carrier protein diphtheria toxin cross-reacting material 197 (DT-CRM 197) and the adjuvant C34 are provided. Antibodies generated by immunogenic compositions disclosed herein further neutralize at least one of the antigens Globo H, stage-specific embryonic antigen-3 (SSEA-3) and stage-specific embryonic antigen-4 (SSEA-4). Therapeutics against breast cancer stem cells comprising immunogenic compositions comprising Globo H, SSEA-3 or SSEA-4 conjugated with DT-CRM 197.

公开(公告)号：MX2010013932A

公开(公告)日：2013-03-01

申请号：MX2010013932

申请日：2009-08-06

Applicant: ACADEMIA SINICA

Inventor： WONG CHI-HUEY ,  WU CHUNG-YI ,  YU JOHN ,  YU ALICE

IPC: A61K39/00 ,  A61K39/385

Abstract: Se proporcionan composiciones inmunogénicas, vacunas de cáncer y métodos para tratar cáncer. Se proporcionan composiciones que comprenden: (a) un glicano tal como Globo H o un fragmento inmunogénico del mismo, en donde el glicano está conjugado con una proteína portadora mediante un enlazador tal como para-nitrofenilo; y (b) un adyuvante que comprende glucolípido capaz de enlazar CDId sobre una célula dendrítica, tal como un derivado de a-galactosilceramida, en donde la composición inmunogénica induce una respuesta inmune que induce un nivel relativo más alto de anticuerpos de isotipo IgG como es comparado con los anticuerpos de isotipo IgM. Se proporcionan composiciones inmunogénicas que comprenden el material de reacción cruzada de toxina de difteria de proteína portadora 197 (DT-CRM 197) y el adyuvante C34. Los anticuerpos generados por las composiciones inmunogénicas divulgadas en la presente además neutralizan por lo menos uno de los antígenos Globo H, antígeno embriónico específico de etapa-3 (SSEA-3) y antígeno embriónico específico de etapa-4 (SSEA-4). Terapias contra células madre de cáncer de seno que comprenden composiciones inmunogénicas que comprenden Globo H, SSEA-3 o SSEA-4 conjugado con DT-CRM 197.

公开(公告)号：NZ590140A

公开(公告)日：2012-07-27

申请号：NZ59014009

申请日：2009-08-06

Applicant: ACADEMIA SINICA

Inventor： WONG CHI-HUEY ,  WU CHUNG-YI ,  YU ALICE ,  YU JOHN

IPC: A61K39/00 ,  A61K39/385

Abstract: Disclosed is an immunogenic composition comprising: (a) a glycan conjugate including a carrier protein, and a glycan including Globo H, an immunogenic fragment thereof, or stage-specific embryonic antigen-4 (SSEA-4), wherein the glycan is conjugated with the carrier protein through a linker; and (b) an adjuvant including C34, wherein C34 is defined in the specification. Also disclosed is the use of the immunogenic composition in the manufacture of a medicament for the treatment or inhibition of tumor growth, wherein administration of medicament induces au immune response that produces a higher relative level of IgG isotype antibodies as compared to IgM isotype antibodies.

公开(公告)号：CA2728341A1

公开(公告)日：2010-01-14

申请号：CA2728341

申请日：2009-08-06

Applicant: ACADEMIA SINICA

Inventor： WONG CHI-HUEY ,  WU CHUNG-YI ,  YU ALICE ,  YU JOHN

IPC: A61K39/00 ,  A61K39/385

Abstract: Immunogenic compositions, cancer vaccines and methods for treating cancer are provided. Compositions comprising: (a) a glycan such as Globo H or an immunogenic fragment thereof, wherein the glycan is conjugated with a carrier protein by a linker such as para-nitrophenyl; and (b) an adjuvant comprising glycolipid capable of binding CDId on a dendritic cell, such as an a-galactosyl-ceramide derivative, wherein the immunogenic composition induces an immune response that induces a higher relative level of IgG isotype antibodies as compared to IgM isotype antibodies, are provided. Immunogenic compositions comprising the carrier protein diphtheria toxin cross-reacting material 197 (DT-CRM 197) and the adjuvant C34 are provided. Antibodies generated by immunogenic compositions disclosed herein further neutralize at least one of the antigens Globo H, stage-specific embryonic antigen-3 (SSEA-3) and stage-specific embryonic antigen-4 (SSEA-4). Therapeutics against breast cancer stem cells comprising immunogenic compositions comprising Globo H, SSEA-3 or SSEA-4 conjugated with DT-CRM 197.

公开(公告)号：WO2012061113A3

公开(公告)日：2012-06-21

申请号：PCT/US2011057637

申请日：2011-10-25

Applicant: ACADEMIA SINICA TAIWAN ,  YU JOHN ,  YU ALICE ,  WU H C ,  WANG SHENG-HUNG ,  I-JU CHEN

Inventor： YU JOHN ,  YU ALICE ,  WU H C ,  WANG SHENG-HUNG ,  I-JU CHEN

IPC: C07K5/10 ,  A61K38/07 ,  A61K38/10 ,  A61K49/14 ,  A61P35/00 ,  C07K7/08

CPC classification number: G06F19/16 ,  A61K9/127 ,  A61K9/1271 ,  A61K31/337 ,  A61K31/475 ,  A61K31/496 ,  A61K31/704 ,  A61K38/10 ,  A61K45/06 ,  A61K47/48815 ,  A61K47/6911 ,  A61K49/0043 ,  A61K49/0056 ,  A61K51/1234 ,  C07K5/1024 ,  C07K7/08 ,  G01N2333/47 ,  G01N2500/04 ,  A61K2300/00

Abstract: Cancer-targeting peptides having a PX1LX2 motif, in which X1 is His or an amino acid residue with a hydrophobic side chain and X2 is Pro, Phe, or Trp. Also disclosed herein are conjugates containing the cancer-targeting peptides and uses thereof in cancer treatment and diagnosis.

Abstract translation: 具有PX1LX2基序的癌症靶向肽，其中X1是His或具有疏水侧链的氨基酸残基，并且X2是Pro，Phe或Trp。 本文还公开了含有癌症靶向肽的缀合物及其在癌症治疗和诊断中的用途。