公开(公告)号：GB2296773B

公开(公告)日：1998-12-09

申请号：GB9526464

申请日：1995-12-22

Applicant: AGENCY IND SCIENCE TECHN

Inventor： MIZUTANI FUMIO ,  HIRATA YOSHIKI ,  YABUKI SOICHI

IPC: G01N27/327 ,  C12Q1/00

公开(公告)号：GB2296773A

公开(公告)日：1996-07-10

申请号：GB9526464

申请日：1995-12-22

Applicant: AGENCY IND SCIENCE TECHN

Inventor： MIZUTANI FUMIO ,  HIRATA YOSHIKI ,  YABUKI SOICHI

IPC: G01N27/327 ,  C12Q1/00

Abstract: An enzyme electrode comprises a polyion complex membrane of an immobilised enzyme formed on an electrode. The polyion complex membrane comprises polycations and polyanions. The polycations are polymers having cationic groups, examples being polylysine, poly(ethyleneimine), poly(dimethylaminoethyl methacrylate) and quaternarized poly (vinyl pyrrolidone). The polyanions are polymers having anionic groups, examples being poly(styrene sulfonic acid), polyglutamic acid, perfluorinated ionomer, or poly(ester-sulfonic acid). The electrode is made by applying an aqueous solution of polycations (or polyanions) to the electrode, applying an aqueous solution of the enzyme, applying an aqueous solution of polyanions (or polycations), and drying. Lactic acid may be determined using lactate oxidase as the enzyme.

公开(公告)号：JPH08178886A

公开(公告)日：1996-07-12

申请号：JP32258594

申请日：1994-12-26

Applicant: AGENCY IND SCIENCE TECHN

Inventor： MIZUTANI FUMIO ,  HIRATA YOSHIKI ,  YABUKI SOICHI

IPC: G01N27/327 ,  C12Q1/00

Abstract: PURPOSE: To improve an enzyme electrode to obtain a high sensitivity, quick response, high durability by mixing a polycation aqueous solution and a polyanion aqueous solution on a substrate electrode, drying the same and constituting a polyion complex film. CONSTITUTION: After a substrate electrode is pretreated, e.g. polished, etc., a polycation aqueous solution (or polyanion aqueous solution) is dropped onto the surface of the electrode, then an aqueous solution of an enzyme to be solidified is dropped. Finally, a polyanion aqueous solution (or polycation aqueous solution) is dropped. In the final dropping process, a polyion complex taking in the enzyme precipitates on the substrate electrode. When the aqueous solution is air-dried, an enzyme electrode with the solidified enzyme film on the substrate electrode is obtained. The ratio of the polycation and polyanion is preferably equivalent to that required for forming the complex. Moreover, the amount of the enzyme to be solidified is 10% or more, and desirably 30-100% to the polyion complex.