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公开(公告)号：JPH04144677A
公开(公告)日：1992-05-19
申请号：JP27003290
申请日：1990-10-08
Applicant: AGENCY IND SCIENCE TECHN
Inventor： HOSONO KUNIAKI
Abstract: PURPOSE:To enable extraction of an intracellular substance from yeast cells at a low cost without any troublesome operation by applying an osmotic pressure to shock yeast fungi under weak alkaline conditions without mechanically crushing the yeast fungi. CONSTITUTION:Prescribed common salt is added to a culture medium such as YPD culture medium (containing 1% yeast extract, 2% peptone and 2% glucose) for yeasts to culture a halotolerant yeast such as Zygosaccharomyces.rouxii and further a yeast such as Saccharomyces.cerevisiae with low halotolerance and the yeast fungi are then collected and subsequently washed with a saline solution at a concentration about equal to that of the common salt in the culture medium. The washed fungal cells are then suspended in a buffer solution such as alkaline sodium hydrogencarbonate.sodium carbonate buffer solution at pH about 9-10 without containing the common salt and incubated to carry out osmotic pressure shock. Thereby, simple operation can be performed without contamination by cell walls, cell cross sections, etc., and the fungal cells in a large amount can be treated at a low cost due to no use of a special chemical.
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公开(公告)号：JPH0614766A
公开(公告)日：1994-01-25
申请号：JP23349191
申请日：1991-09-12
Applicant: AGENCY IND SCIENCE TECHN , KUMAMOTO PREFECTURE
Inventor： HOSONO KUNIAKI , NISHIMURA KATANORI , NAKAGAWA MASARU , HACHIMAN NORIYOSHI
Abstract: PURPOSE:To obtain a new fused strain capable of providing a soft type SHOCHU (low-class distilled spirit), having good aroma and manifesting the acid resistance, high-temperature fermenting properties, excellent aroma and high yield of alcohol by subjecting mutual strains belonging to Saccharomyces.cerevisiae to protoplast fusion. CONSTITUTION:The objective fused yeast is obtained by mutually subjecting strains belonging to Saccharomyces.cerevisiae to protoplast fusion, e.g. Saccharomyces.cerevisiae KS2 (FERM P-12233). This fused yeast is obtained by preparing a yeast fungal cell suspension from, e.g. respective lysine-requiring mutant strains of Saccharomyces.cerevisiae KUMAMOTO yeast for SAKE (Japanese rice wine) and Saccharomyces.cerevisiae S-4, then treating each with a protoplast preparing solution containing a yeast cell wall digesting enzyme, subsequently mixing equal amounts of both the protoplasts, then adding a fusion agent, carrying out the cell fusion, further culturing the protoplast mixture solution and separating the formed colony.
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公开(公告)号：JPS62192303A
公开(公告)日：1987-08-22
申请号：JP3602486
申请日：1986-02-20
Applicant: AGENCY IND SCIENCE TECHN
Inventor： HOSONO KUNIAKI , SUZUKI HIDEO
Abstract: PURPOSE:A novel mold mycelial growth inhibitor, consisting of a peptide having specific acyl groups, capable of exhibiting improved effect on growth inhibition of mold mycelia and particularly useful as an agricultural chemical against Piricularia oryzae (blast) which is hardly controlled. CONSTITUTION:An inhibitor consisting of a peptide having acyl groups expressed by the formula (R is C10H21, C11H23 or C12H25) having inhibitory action on cycle adenosine-3',5'-monophosphate phosphodiesterase. The above-mentioned compound expressed by the formula is obtained by cultivating Bacillus subtilis C-756 (FERM-P No.6785) separated from soil normally by an aerobic culture method and recovering the produced peptide produced in the cultivation filtrate. The above-mentioned inhibitor contains 0.02-1.0wt% above-mentioned compound as an active ingredient and is used in the normally used form of a solution, e.g. aqueous solution, etc., a mixture with a carrier powder, etc., by a spraying or coating method, etc. The inhibitor can be utilized as chemical for various blights or damages, e.g. agricultural chemical, medicine or antimicrobial agent (antifungal agent).
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公开(公告)号：JPH089994A
公开(公告)日：1996-01-16
申请号：JP17362492
申请日：1992-05-21
Applicant: AGENCY IND SCIENCE TECHN
Inventor： YAMAZAKI YUKINAE , HOSONO KUNIAKI , TOMIZUKA NOBORU
Abstract: PURPOSE:To obtain the subject optically high-purity compound useful as a synthetic raw material, etc., for physiologically active substances, organometallic polymeric complexes by reducing acetylferrocene with a yeast of the genus Candida, a yeast of the genus Saccharomyces, a bacterium of the genus Nocardia, etc. CONSTITUTION:This method for producing an optically active dextro-rotatory (+)-(S)-1- hydroxyethylferrocene is to use a microbial cell of a yeast of the genus Candida (e.g. Candica tropicalis IFO199), a yeast of the genus Saccharomyces (e.g. Saccharomyces rosei IFO428) or a bacterium of the genus Nocardia (e.g. Nocardia erythropolis IAM12122), inoculate the seed culture into a culture medium, carry out the shaking culture at 28 deg.C for 23hr on a rotary shaker, then centrifuge the resultant cultured product, collect the microbial cell, add the collected microbial cell into a solution prepared by adding a solution of acetylferrocene to a 0.1M phosphoric acid buffer solution (pH 7.5) containing glucose, performing the ultrasonic treatment and uniformly dispersing the acetylferrocene therein, shake the resultant mixture solution at 28 deg.C for 69hr and reduce the acetylferrocene. The obtained opt ically active dextro- rotatory (+)-(S)-1-hydroxyethylferrocene is useful as a synthetic raw material, etc., for physiologically active substances or organometallic polymeric complexes.
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Patent Agency Ranking
公开(公告)号：JPH0471499A
公开(公告)日：1992-03-06
申请号：JP18108890
申请日：1990-07-09
Applicant: AGENCY IND SCIENCE TECHN
Inventor： YAMAZAKI YUKINAE , HOSONO KUNIAKI , SHIRAKI MASARU
IPC: C12P41/00
Abstract: PURPOSE:To prepare the subject complex useful as a raw material for asymmetric organic synthesis reagents in a simple process in a good yield by asymmetrically acylating the racemates of a specific compound with a lipase and subsequently hydrolyzing the racemates. CONSTITUTION:The racemates of a compound of the formula I (R1 is alkyl, alkoxy) is asymmetrically acylated with a lipase, and the obtained ester is separated from the unreacted alcohol, followed by hydrolyzing the ester, whereby the dextro notatory optically active isomer and the levorotatory optically active isomer are obtained. The lipase is preferably a lipase originated from e.g. readily available Pseudomonas fluorescence.
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公开(公告)号：JPH0361481A
公开(公告)日：1991-03-18
申请号：JP19677589
申请日：1989-07-31
Applicant: AGENCY IND SCIENCE TECHN
Inventor： KAJIWARA SHIGERU , HOSONO KUNIAKI , MAEDA HIDEKATSU , IIDA MITSUGI , KIMURA KEIKO
Abstract: PURPOSE:To make possible to produce dehydrogenating enzyme for formic acid having specific physicochemical properties such as a small Km value to formic acid and NAD by culturing a microorganism belonging to genus Paracoccus in medium and accumulating in the cultured substance. CONSTITUTION:A microorganism belonging to genus Paracoccus having formic acid as an only carbon source such as Paracoccus 12-A strain (FERM P-10835) is cultured in a medium containing formic acid as main carbon source, ammonium salt, etc., as nitrogen source, and phosphoric acid source, etc., as inorganic salt. Next, the cultivation is finished and the microbial cell is collected from the cultured solution by means of centrifugation, etc., then enzyme is extracted into a buffer solution with crushing the microbial cell by means of supersonic cell crusher, etc., thus purified with a salting-out method, etc., to afford the objective dehydrogenating enzyme for formic acid. Resultant enzyme has the following properties; active temperature range: 25-70 deg.C, active pH range: 5.0-11, molecular weight: about 100,000, Km value to formic acid: 5.0mM, Km value to NAD: 0.036mM, stability to temperature:
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公开(公告)号：JPH01281092A
公开(公告)日：1989-11-13
申请号：JP11188388
申请日：1988-05-09
Applicant: AGENCY IND SCIENCE TECHN
Inventor： YAMAZAKI YUKINAE , HOSONO KUNIAKI , TOMIZUKA NOBORU
Abstract: PURPOSE:To readily and efficiently obtain the optical isomer of 1- hydroxyethylferrocene by reducing acetyl-ferrocene with a specific microbial cell or treating racemic body of 1-hydroxyethylferrocene with a specific enzyme. CONSTITUTION:Among the optical isomer of 1-hydroxyethylferrocene, the dextro- rotatory (+)-(S)-1-hydroxyethylferrocene is obtained by reducing acetylferrocene using a microbial cell selected from yeasts belonging to Candida and Saccharomyces and a bacterium belonging to Nocardia and the levo-rotatory (-)-(R)-1-hydroxyethylferrocene is obtained by treating the racemic body of 1-hydroxyethelferrocene with an alcoholdehydrogenase of horse liver.
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公开(公告)号：JPH0975084A
公开(公告)日：1997-03-25
申请号：JP25450595
申请日：1995-09-07
Applicant: AGENCY IND SCIENCE TECHN , HIGETA SHOYU KK
Inventor： ITO HIROKO , MATSUI IKUO , ISHIKAWA KAZUHIKO , HIRANO TAKASHI , HOSONO KUNIAKI , TAKAGI HIROAKI , TANAKA AKIMITSU
Abstract: PROBLEM TO BE SOLVED: To obtain a new modified synthetic enzyme in which tyrosine constituting the active center of the region A of a cyclodextrin-synthesizing enzyme is replaced with another amino acid, and which gives the reaction product greatly high in the amount of the synthesized α-cyclodextrin. SOLUTION: This new modified cyclodextrin-synthesizing enzyme wherein the 100-tyrosine constituting the active center of the region A of a cyclodextrin- synthesizing enzyme having an amino acid sequence of the formula is replaced with another amino acid such as tryptophane, phenylalanine, leucine or aspartic acid. The action of the enzyme on a substrate such as starch can give the reaction product greatly high in the content of the α-cyclodextrin. The modified enzyme is obtained by extracting the chromosome from Bacillus macerans IAM 1243, multiplying the chromosome with PCR using a synthesized primer containing the base sequence of the cyclodextrin-synthesizing enzyme, subjecting the multiplied chromosome to a site-specific mutation so as to replace the tyrosine of the active center of the obtained gene with another amino acid.
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公开(公告)号：JPH0937797A
公开(公告)日：1997-02-10
申请号：JP21404395
申请日：1995-07-31
Applicant: AGENCY IND SCIENCE TECHN
Inventor： ITO HIROKO , HIRANO TAKASHI , HOSONO KUNIAKI
Abstract: PROBLEM TO BE SOLVED: To obtain the subject new primer consisting of oligonucleotides having specific base sequences respectively, capable of amplifying the 18S ribosome RNA of plant worm, determining and analyzing quickly the base sequence and identifying the plant worm's strain. SOLUTION: This new primer consists of oligonucleotides having base sequences of formula I and formula II, respectively, and has the following advantages: the 18S ribosome RNA gene of plant worm can be amplified by PCR process; the base sequence of the gene can be determined and analyzed quickly; the plant worm's strain capable of producing pharmacologically active substance can be identified based on its genetic characteristics; and exploration efficiency of new physiologically active substance-productive bacteria can be remarkably improved. As the plant worm (e.g. Cordyceps sinensis) belongs to Ascomycetes, this new primer is obtained by the following process: several kinds of partial base sequences are synthesized based on the base sequence of the 18S ribosome RNA gene of a strain (e.g. Sclerotinia sclerotiorum) as other Ascomycetes, and from these partial base sequences, one that enables amplification of the plant worm's gene is selected.
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