公开(公告)号：JPH0549475A

公开(公告)日：1993-03-02

申请号：JP29184891

申请日：1991-08-20

Applicant: AGENCY IND SCIENCE TECHN

Inventor： MIYAKE MASATO ,  ASADA YASUO ,  SHIRAKI MASARU ,  KATO SAKAE

IPC: C12N9/16 ,  C12R1/89

Abstract: PURPOSE:To simply obtain a heat resistant restriction enzyme having high active purity in high yield by subjecting a restriction enzyme solution to heat treatment for a time when an endonuclease contained as impurity is substantially deactivated at the lowest temperature at which an objective heat resistant restriction enzyme starts to deactivate. CONSTITUTION:A restriction enzyme-containing solution is subjected to heat treatment at the lowest temperature at which the objective heat resistant enzyme starts to deactivate for a time when an endonuclease contained as an impurity is substantially deactivated. The solution is subjected to heat treatment at 60 deg.C for 10min in the case of a new heat resistant restriction enzyme Sel I (whose enzymatic properties are shown in the table) derived from thermophilic Synechococcus elongatus (FERM P12393).

公开(公告)号：JPH03127992A

公开(公告)日：1991-05-31

申请号：JP26376689

申请日：1989-10-09

Applicant: AGENCY IND SCIENCE TECHN ,  CCI KK

Inventor： KOSUGI YOSHIJI ,  SHIRAKI MASARU ,  KUNIEDA TSUTOMU ,  MURASE HIRONORI

IPC: C12P7/62 ,  C12P7/64

Abstract: PURPOSE:To decrease the free fatty acid content in an ester after reaction by reacting a fatty acid with an alcohol in the presence of an immobilized lipase. CONSTITUTION:An ester is produced by reacting a fatty acid with an alcohol. The above process is carried out by reacting in the presence of an immobilized lipase having an anion-exchange group on the carrier surface and then in the presence of a lipase immobilized to a hydrophobic carrier free from anion exchange group on the carrier surface. The lipase used in the above process is an enzyme capable of bonding an acyl-enzyme (R-COO-E) or (R-COS-E) by bonding a fatty acid (R-COOH) with an enzyme (HO-E) or (HS-E) and any kind of enzyme such as phospholipase, lipoprotein lipase, protease, etc., can be used provided that the enzyme has the above function. The immobilized lipase can be prepared by contacting a lipase solution with an anion exchange material or a hydrophobic carrier.

公开(公告)号：JPH02138975A

公开(公告)日：1990-05-28

申请号：JP29315388

申请日：1988-11-19

Applicant: AGENCY IND SCIENCE TECHN ,  RAKUTOU KASEI KOGYO KK

Inventor： KOSUGI YOSHIJI ,  TANAKA HIDEOKI ,  SHIRAKI MASARU ,  TAKAHASHI KATSUO ,  MAEDA ISAO ,  SHIMOTOMAI KOICHI

IPC: C12N11/08

Abstract: PURPOSE:To prepare a large amount of a highly active immobilized lipase by bringing a solution, regulated to >=0.1 ionic strength and containing 40-70vol.% water-miscible polar solvent added thereto into contact with an anion exchanger. CONSTITUTION:A lipase enzymic solution, regulated to

公开(公告)号：JPS6322803A

公开(公告)日：1988-01-30

申请号：JP31283586

申请日：1986-12-26

Applicant: AGENCY IND SCIENCE TECHN

Inventor： HIRANO TAKASHI ,  OHASHI SHINICHI ,  MORIMOTO SATOSHI ,  SHIRAKI MASARU ,  TSUDA KEISHIRO ,  KOBAYASHI TOMOO ,  TSUKAGOSHI SHIGERU

IPC: A61K31/765 ,  A61K45/00 ,  A61K47/48 ,  A61P35/00 ,  C08F8/30

Abstract: PURPOSE:To obtain a copolymer having excellent slow-releasing property of a carcinostatic substance, low toxicity and improved carcinostatic effect, by reacting a carcinostatic substance to a divinyl ether-maleic anhydride copolymer and hydrolyzing the reaction product. CONSTITUTION:A divinyl ether-maleic anhydride copolymer of formula I (n is a value corresponding to the copolymer molecular weight of 2,000-15,000) is made to react with a carcinostatic substance having hydroxyl group or amino group (e.g. 5-fluorouridine, 1-beta-D-arabinofuranosylcytosine) in the presence of an organic solvent (e.g. N-methylpyrrolidone) at a rate to give a content of usually 5-40wt%. The reaction product is hydrolyzed and optionally converted to a salt to obtain the objective copolymer of formula II (R is residue obtained by removing one H atom from the hydroxyl group or amino group of the above carcinostatic substance). EFFECT:The carcinostatic activity is promoted by the synergistic effect with the carcinostatic activity of the copolymer.

公开(公告)号：JPH0471499A

公开(公告)日：1992-03-06

申请号：JP18108890

申请日：1990-07-09

Applicant: AGENCY IND SCIENCE TECHN

Inventor： YAMAZAKI YUKINAE ,  HOSONO KUNIAKI ,  SHIRAKI MASARU

IPC: C12P41/00

Abstract: PURPOSE:To prepare the subject complex useful as a raw material for asymmetric organic synthesis reagents in a simple process in a good yield by asymmetrically acylating the racemates of a specific compound with a lipase and subsequently hydrolyzing the racemates. CONSTITUTION:The racemates of a compound of the formula I (R1 is alkyl, alkoxy) is asymmetrically acylated with a lipase, and the obtained ester is separated from the unreacted alcohol, followed by hydrolyzing the ester, whereby the dextro notatory optically active isomer and the levorotatory optically active isomer are obtained. The lipase is preferably a lipase originated from e.g. readily available Pseudomonas fluorescence.

公开(公告)号：JPH0292282A

公开(公告)日：1990-04-03

申请号：JP24313188

申请日：1988-09-28

Applicant: AGENCY IND SCIENCE TECHN

Inventor： KOSUGI YOSHIJI ,  TANAKA HIDEOKI ,  SHIRAKI MASARU

IPC: C12N11/08

Abstract: PURPOSE:To obtain immobilized lipase capable of using in the presence of a fatty acid having high concentration and adapted to a counter current type reaction system by immobilizing a lipase so as to leave an ununited anion exchange group of the lipase on a fine powder hydrophobic carrier containing the anion exchange group. CONSTITUTION:An anion exchange group (e.g., tert. amino group or quaternary ammonium group) is introduced into a substrate of anion exchange resin (e.g., styrene/divinylbenzene copolymer) to prepare a fine particle hydrophobic carrier containing an anion exchange group and having 0.02-0.3mm particle size. Then the fine powder hydrophobic carrier is brought into contact with a lipase to immobilize the lipase so as to leave ununited anion exchange group of the lipase. Since lipase molecule exists together with anion exchange group in the resultant immobilized lipase, the above-mentioned reaction can be carried out in the presence of a fatty acid having high concentration and the immobilized lipase is preferably used in field of oils and fats, etc.

公开(公告)号：JPH0672959A

公开(公告)日：1994-03-15

申请号：JP13376291

申请日：1991-03-28

Applicant: AGENCY IND SCIENCE TECHN ,  BOOSOO YUSHI KK

Inventor： KOSUGI YOSHIJI ,  SHIRAKI MASARU ,  AZUMA NAOTERU

IPC: C07C69/587 ,  C12P7/64

Abstract: PURPOSE:To provide the subject novel compound useful for the prevention of circulatory diseases such as hypercholesterolemia and thrombosis, for medical experiments, for the research of physiological functions, etc. CONSTITUTION:A compound of formula I (R is group of formula II). The compound of formula I is obtained by mixing eicosapentaenoic acid triglyceride with glycerol in a prescribed ratio, and subsequently simultaneously performing the contact reaction of the prepared substrate with an immobilized lipase and the dehydrative treatment of the reaction solution.

公开(公告)号：JPH05331105A

公开(公告)日：1993-12-14

申请号：JP27447391

申请日：1991-07-24

Applicant: AGENCY IND SCIENCE TECHN ,  BOOSOO YUSHI KK

Inventor： KOSUGI YOSHIJI ,  SHIRAKI MASARU ,  AZUMA NAOTERU

IPC: C07C69/587 ,  C12P7/64

Abstract: PURPOSE:To provide a new docosahexaenoic acid triglyceride which is useful as a functional food, and in the study of physiological functions of docosahexaenoic acid, reduction in cholesterol, prophylaxis for cardiovascular diseases such as thrombosis and medical experiments. CONSTITUTION:Docosahexaenoic acid of formula I (R is formula II). The compound is obtained by preparing a substrate as a mixture of docosahexaenoic acid or its alcohol ester and glycerol at the stoichiometric ratio or close thereto. Then the substrate is brought into contact with immobilized lipase, preferably the immobilized lipase from Candida, and the reaction mixture is subjected to water or alcohol removal to give a high concentration of the glyceride. The reaction is carried out at 30 to 60 deg.C in the reaction system under the atmosphere where the air is replaced with an inert gas and the water or alcohol removal is effected under vacuum or by passing an inert gas through the reaction mixture. The concentration operation is continued until the water content becomes lower than 100ppm in the reaction system.

公开(公告)号：JPH05328962A

公开(公告)日：1993-12-14

申请号：JP33750291

申请日：1991-10-22

Applicant: AGENCY IND SCIENCE TECHN ,  BOOSOO YUSHI KK

Inventor： KOSUGI YOSHIJI ,  SHIRAKI MASARU ,  AZUMA NAOTERU

IPC: C12M1/40 ,  C12P7/64

Abstract: PURPOSE:To obtain a continuous synthesizer for an ester capable of synthesizing the ester in high yield and a method for continuous synthesis thereof. CONSTITUTION:A fixed bed reactor 1 containing an immobilized lipase is connected through a circulating pump 3 to a dehydrating vessel 2 in a loopy form. The resultant reactional systems are arranged in at least two stages (A) and (B). The mutual adjacent reactional systems (A) and (B) are connected with a transfer pipe 5 having a transfer pipe 4 placed therein and an acid feed source 8 and an alcohol feed source 9 are connected to the first reactional system (A). The final reactional system (B) is equipped with a taking outlet 12 for the resultant product.

公开(公告)号：JPH06181739A

公开(公告)日：1994-07-05

申请号：JP9345391

申请日：1991-03-29

Applicant: AGENCY IND SCIENCE TECHN

Inventor： KOSUGI YOSHIJI ,  SHIRAKI MASARU

IPC: C12M1/00 ,  C12M1/02 ,  C12N11/04 ,  C12P7/64

Abstract: PURPOSE:To provide a binary phase bioreactor for efficiently performing a reaction using an immobilized enzyme. CONSTITUTION:The objective binary phase immobilized enzyme reactor is provided with stationary tanks 2, 8 at the top and the bottom and two or more stirring tanks 4 at the intermediate part. These tanks are separated from each other with liquid-permeable partition plates 5 having micropores inhibiting the passage of the immobilized enzyme. The stirring tanks contain immobilized enzyme. A pipe 1 for discharging a product having low specific gravity is attached to the top end of the top stationary tank, the top stirring tank is furnished with a pipe 3 for supplying a substrate having high specific gravity, the bottom stirring tank is furnished with a pipe 7 for supplying a substrate having low specific gravity and a pipe 9 for discharging a product having high specific gravity is attached to the lower end of the bottom stationary tank. A pulse generator 6 is connected to the bottom of the bottom stationary tank.