公开(公告)号：GB2433449B

公开(公告)日：2011-01-26

申请号：GB0625308

申请日：2006-12-19

Applicant: AGILENT TECHNOLOGIES INC

Inventor： YIN HONGFENG ,  KILLENN KEVIN ,  BRENNEN REID

IPC: B01L99/00 ,  G01N30/20 ,  G01N30/60 ,  G01N30/72

Abstract: Fluidic separation devices and methods with reduced sample broadening are provided. A column is located downstream from a holding chamber, and a flow providing means provides fluid flow effective to convey a sample along a flow path that extends from the holding chamber into the separation column. The sample is typically focused in the flow path upstream from the separation column. Optionally, the invention may be employed with electrospray mass spectrometry and in microfluidic applications.

公开(公告)号：GB2433449A

公开(公告)日：2007-06-27

申请号：GB0625308

申请日：2006-12-19

Applicant: AGILENT TECHNOLOGIES INC

Inventor： YIN HONGFENG ,  KILLENN KEVIN ,  BRENNEN REID

IPC: B01L99/00 ,  G01N30/16 ,  B01D15/14 ,  B81B1/00 ,  G01N30/60

Abstract: Fluidic separation devices (10) and methods with reduced sample broadening are provided. A column (25) is located downstream from a holding chamber (53), and a flow providing means provides fluid flow effective to convey a sample along a flow path that extends from the holding chamber (53) into the separation column (25). The sample is typically focused in the flow path upstream from the separation column (25). Optionally, the invention may be employed with electrospray mass spectrometry and in microfluidic applications. The means for focusing the sample may preferably be achieved by using material that makes holding chamber less sample retentive than the separation column, providing a heat source to the holding chamber and/or having an inlet conveying fluid downstream from the holding chamber and upstream from the separation column.