公开(公告)号：WO1994024286A1

公开(公告)日：1994-10-27

申请号：PCT/US1994003463

申请日：1994-03-30

Applicant: ALLIEDSIGNAL INC.

Inventor： ALLIEDSIGNAL INC. ,  GOLDBERG, Ina ,  ALLENZA, Paul ,  LUPTON, Francis, Stephen

IPC: C12N15/52

CPC classification number: C12N9/0044

Abstract: The present invention provides a recombinant plasmid adapted for transformation of a host microorganism, the plasmid comprising a plasmid vector into which a polydeoxyribonucleotide segment that codes for ammonia monooxygenase and hydroxylamine oxidoreductase has been inserted, wherein the resulting transformant exhibits at least 50 % of the overall level of ammonia monooxygenase and hydroxylamine oxidoreductase activity of the donor. Also provided are an isolated polydeoxyribonucleotide segment that encodes ammonia monooxygenase and hydroxylamine oxidoreductase, and a transformant containing the ammonia monooxygenase and hydroxylamine oxidoreductase gene segments.

Abstract translation: 本发明提供了适于转化宿主微生物的重组质粒，该质粒包含质粒载体，其中插入了编码氨单加氧酶和羟胺氧化还原酶的多脱氧核糖核苷酸区段，其中所得转化体显示总体至少50％ 供体的氨单加氧酶水平和羟胺氧化还原酶活性。 还提供了编码氨单加氧酶和羟胺氧化还原酶的分离的多脱氧核糖核苷酸片段，以及含有氨单加氧酶和羟胺氧化还原酶基因片段的转化体。

公开(公告)号：EP0449839A1

公开(公告)日：1991-10-09

申请号：EP89911317.0

申请日：1989-09-06

Applicant: AlliedSignal Inc.

Inventor： GOLDBERG, Ina ,  SALERNO, Anthony, Joseph

IPC: C12N15 ,  C12N1 ,  C12P21 ,  C12R1

CPC classification number: C12N15/67 ,  C12N15/11 ,  C12N15/70

Abstract: La présente invention concerne une nouvelle souche bactérienne permettant d'améliorer l'expression de gènes hétérologues.