公开(公告)号：WO2006118857A3

公开(公告)日：2007-02-22

申请号：PCT/US2006015491

申请日：2006-04-20

Applicant: AMNIS CORP ,  ORTYN WILLIAM ,  BASIJI DAVID ,  LYNCH DAVID

Inventor： ORTYN WILLIAM ,  BASIJI DAVID ,  LYNCH DAVID

IPC: G01N33/53

CPC classification number: G01N33/505 ,  G01J3/2889 ,  G01J3/36 ,  G01J3/4406 ,  G01N15/1429 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/53 ,  G01N21/6428 ,  G01N21/6456 ,  G01N21/6458 ,  G01N33/5005 ,  G01N2015/1006 ,  G01N2015/1497 ,  G01N2021/6421 ,  G01N2021/6441 ,  G06K9/00127

Abstract: Aspects of the present invention encompass the collection of multispectral images from a population of objects, and the analysis of the collected images to measure at least one characteristic of the population, using photometric and/or morphometric features identifiable in the collection of images. In an exemplary application, the objects are biological cells. In a particularly preferred, but not limiting implementation, the plurality of images for each individual object are collected simultaneously. In an empirical study, the characteristic being measured involves the synapse between conjugated cells. The conjugated cells may represent a subpopulation of the overall population of objects that were imaged. In a particularly preferred, yet not limiting embodiment, the present invention enables the quantization of the redistribution of cellular molecules due to the conjugation of different biological cells. Significantly, such quantization is not feasible with standard microscopy and flow cytometry.

Abstract translation: 本发明的方面包括从对象群体收集多光谱图像，以及使用在图像集合中可识别的光度和/或形态测量特征来分析收集的图像以测量群体的至少一个特征。 在示例性应用中，对象是生物细胞。 在特别优选但不是限制的实现中，用于每个单独对象的多个图像被同时收集。 在实证研究中，所测量的特征涉及共轭细胞之间的突触。 共轭细胞可以代表成像对象的总体群体的亚群。 在特别优选的但不是限制性的实施方案中，本发明使得由于不同生物细胞的共轭而能够量化细胞分子的再分布。 值得注意的是，这种量化在标准显微镜和流式细胞术中是不可行的。

公开(公告)号：EP1886139A4

公开(公告)日：2008-07-16

申请号：EP06758550

申请日：2006-04-20

Applicant: AMNIS CORP

Inventor： ORTYN WILLIAM ,  BASIJI DAVID ,  LYNCH DAVID

IPC: G01N33/53

CPC classification number: G01N33/505 ,  G01J3/2889 ,  G01J3/36 ,  G01J3/4406 ,  G01N15/1429 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/53 ,  G01N21/6428 ,  G01N21/6456 ,  G01N21/6458 ,  G01N33/5005 ,  G01N2015/1006 ,  G01N2015/1497 ,  G01N2021/6421 ,  G01N2021/6441 ,  G06K9/00127

Abstract: Aspects of the present invention encompass the collection of multispectral images from a population of objects, and the analysis of the collected images to measure at least one characteristic of the population, using photometric and/or morphometric features identifiable in the collection of images. In an exemplary application, the objects are biological cells. In a particularly preferred, but not limiting implementation, the plurality of images for each individual object are collected simultaneously. In an empirical study, the characteristic being measured involves the synapse between conjugated cells. The conjugated cells may represent a subpopulation of the overall population of objects that were imaged. In a particularly preferred, yet not limiting embodiment, the present invention enables the quantization of the redistribution of cellular molecules due to the conjugation of different biological cells. Significantly, such quantization is not feasible with standard microscopy and flow cytometry.

Abstract translation: 本发明的各方面包括从物体群收集多光谱图像，并且采集所收集图像的分析以使用图像集合中可识别的光度和/或形态学特征来测量群体的至少一个特征。 在示例性应用中，物体是生物细胞。 在特别优选的但非限制性的实现中，同时收集每个单独对象的多个图像。 在实证研究中，被测量的特征涉及共轭细胞之间的突触。 结合的细胞可以代表成像的整个对象群体的亚群。 在一个特别优选的但非限制性的实施方案中，本发明能够量化由于不同生物细胞的缀合而导致的细胞分子的重新分布。 重要的是，这种量化不适用于标准显微镜和流式细胞术。

公开(公告)号：AT520984T

公开(公告)日：2011-09-15

申请号：AT06758550

申请日：2006-04-20

Applicant: AMNIS CORP

Inventor： ORTYN WILLIAM ,  BASIJI DAVID ,  LYNCH DAVID

IPC: G01N33/53

Abstract: Multimodal/multispectral images of a population of cells are simultaneously collected. Photometric and/or morphometric features identifiable in the images are used to separate the population of cells into a plurality of subpopulations. Where the population of cells includes diseased cells and healthy cells, the images can be separated into a healthy subpopulation, and a diseased subpopulation. Where the population of cells does not include diseased cells, one or more ratios of different cell types in patients not having a disease condition can be compared to the corresponding ratios in patients having the disease condition, enabling the disease condition to be detected. For example, blood cells can be separated into different types based on their images, and an increase in the number of lymphocytes, a phenomenon associated with chronic lymphocytic leukemia, can readily be detected.