公开(公告)号：WO2005119325A2

公开(公告)日：2005-12-15

申请号：PCT/US2005/018677

申请日：2005-05-26

Applicant: APERIO TECHNOLOGIES, INC. ,  OLSON, Allen ,  SALIGRAMAL, Kiran ,  SOENKSEN, Dirk, G.

Inventor： OLSON, Allen ,  SALIGRAMAL, Kiran ,  SOENKSEN, Dirk, G.

IPC: G02B7/04

CPC classification number: G06T7/0012 ,  G02B21/365 ,  G06K9/00127 ,  G06K9/036 ,  G06T2207/10016 ,  G06T2207/10056 ,  G06T2207/30024

Abstract: Systems and methods for assessing virtual microscope slide image quality are provided. In order to determine whether a virtual slide image has any out of focus areas and is therefore a candidate for manual inspection, the various focus points used to scan the virtual slide image are used to calculate a best fit surface for the virtual slide image. The distance of each focus point from the best fit surface is then calculated and the largest distance is compared to a predetermined value. If the largest distance from a focus point to the best fit surface is larger than the predetermined value, then the virtual slide image is designated as needing a manual inspection and possible re-scan.

Abstract translation: 提供了评估虚拟显微镜幻灯片图像质量的系统和方法。 为了确定虚拟幻灯片图像是否具有任何偏离焦点区域，因此是用于手动检查的候选者，用于扫描虚拟幻灯片图像的各种对焦点用于计算虚拟幻灯片图像的最佳拟合曲面。 然后计算每个对焦点与最佳拟合表面的距离，并将最大距离与预定值进行比较。 如果从焦点到最佳配合曲面的最大距离大于预定值，则虚拟幻灯片图像被指定为需要手动检查和可能的重新扫描。

公开(公告)号：WO2004079523A2

公开(公告)日：2004-09-16

申请号：PCT/US2004/006013

申请日：2004-02-27

Applicant: APERIO TECHNOLOGIES, INC. ,  EICHHORN, Ole ,  OLSON, Allen

Inventor： EICHHORN, Ole ,  OLSON, Allen

IPC: G06F

CPC classification number: G06T7/0012 ,  G06F19/00 ,  G06F19/321 ,  G06T1/00 ,  G06T2207/10056 ,  G06T2207/20016

Abstract: A system and method for processing and analyzing virtual microscopy digital images ("virtual slides") is provided. The system comprises an algorithm server that maintains or has access to a plurality of image processing and analysis routines. The algorithm server additionally has access to a plurality of virtual slides. The algorithm server executes a selected routine on an identified virtual slide and provides the resulting data. The virtual slide can be accessed locally or remotely across a network. Similarly, the image processing routines can be obtained from local storage or across a network, or both. Advantageously, certain common sub-routines may be stored locally for inclusion in other local or remotely obtained routines. Access to image processing and analysis may be restricted through a monitor process that authenticates requests to process or view virtual slides. Variations in restrictions to images provide a rich diversity in access levels that allow sharing of virtual slides and demonstrations of image processing algorithms.

Abstract translation: 提供了一种用于处理和分析虚拟显微镜数字图像（“虚拟幻灯片”）的系统和方法。 该系统包括维护或访问多个图像处理和分析例程的算法服务器。 算法服务器还可以访问多个虚拟幻灯片。 算法服务器在识别的虚拟幻灯片上执行所选择的例程，并提供所得到的数据。 虚拟幻灯片可以通过网络在本地或远程访问。 类似地，图像处理例程可以从本地存储器或网络或两者获得。 有利地，某些公共子例程可以被本地存储以包含在其他本地或远程获得的例程中。 图像处理和分析的访问可能会通过对进程或查看虚拟幻灯片的请求进行身份验证的监视进程来限制。 对图像限制的变化提供了访问级别丰富的多样性，允许共享虚拟幻灯片和图像处理算法的演示。

公开(公告)号：WO2011072211A2

公开(公告)日：2011-06-16

申请号：PCT/US2010/059861

申请日：2010-12-10

Applicant: APERIO TECHNOLOGIES, INC. ,  OLSON, Allen ,  EICHHORN, Ole

Inventor： OLSON, Allen ,  EICHHORN, Ole

IPC: G06Q50/00 ,  G06T7/00

CPC classification number: G06T7/0012 ,  G06F19/00 ,  G06F19/321 ,  G06T2207/10056 ,  G06T2207/30024

Abstract: A digital slide analysis system comprises an algorithm server that maintains or has access to a plurality of image processing and analysis routines. The algorithm server additionally has access to a plurality of digital slide images. The algorithm server executes a selected routine on an identified digital slide and provides the resulting data. Prior to the application of selected routine, the system employs a digital pre-processing module to create a metadata mask that reduces undesirable image data such that the image data processed by the selected routine has an improved signal to noise ratio. The pre-processing module uses a classifier that may be implemented as a pattern recognition module, for example. Undesirable image data is therefore excluded from the image data that is processed by the digital pathology image processing and analysis routine, which significantly improves the digital pathology image analysis.

Abstract translation: 数字幻灯片分析系统包括维护或访问多个图像处理和分析例程的算法服务器。 算法服务器还可以访问多个数字幻灯片图像。 算法服务器在识别的数字幻灯片上执行选择的例程，并提供结果数据。 在应用选择的例程之前，系统采用数字预处理模块来创建元数据掩码，其减少不期望的图像数据，使得由所选程序处理的图像数据具有改善的信噪比。 预处理模块使用例如可以被实现为模式识别模块的分类器。 因此，不希望的图像数据被排除在由数字病理图像处理和分析程序处理的图像数据中，这显着改善了数字病理图像分析。

公开(公告)号：WO2013165576A1

公开(公告)日：2013-11-07

申请号：PCT/US2013/031045

申请日：2013-03-13

Applicant: APERIO TECHNOLOGIES, INC.

Inventor： ZOU, Yunlu ,  CRANDALL, Greg J. ,  OLSON, Allen

IPC: G02B21/00 ,  G01B9/04

CPC classification number: G02B21/006 ,  G02B21/245 ,  G02B21/361 ,  G02B21/365

Abstract: Systems and methods for capturing a digital image of a slide using an imaging line sensor and a focusing line sensor. In an embodiment, a beam-splitter is optically coupled to an objective lens and configured to receive one or more images of a portion of a sample through the objective lens. The beam-splitter simultaneously provides a first portion of the one or more images to the focusing sensor and a second portion of the one or more images to the imaging sensor. A processor controls the stage and/or objective lens such that each portion of the one or more images is received by the focusing sensor prior to it being received by the imaging sensor. In this manner, a focus of the objective lens can be controlled using data received from the focusing sensor prior to capturing an image of a portion of the sample using the imaging sensor.

Abstract translation: 使用成像线传​​感器和聚焦线传感器捕获幻灯片的数字图像的系统和方法。 在一个实施例中，分束器光学耦合到物镜并且被配置为通过物镜接收样品的一部分的一个或多个图像。 分束器同时将一个或多个图像的第一部分提供给聚焦传感器，并将一个或多个图像的第二部分提供给成像传感器。 处理器控制舞台和/或物镜，使得一个或多个图像的每个部分在被成像传感器接收之前由聚焦传感器接收。 以这种方式，可以在使用成像传感器捕获样本的一部分的图像之前，使用从聚焦传感器接收的数据来控制物镜的焦点。

公开(公告)号：WO2013010023A2

公开(公告)日：2013-01-17

申请号：PCT/US2012/046535

申请日：2012-07-12

Applicant: APERIO TECHNOLOGIES, INC. ,  CRANDALL, Gregory ,  OLSON, Allen

Inventor： CRANDALL, Gregory ,  OLSON, Allen

IPC: G06T7/00 ,  G01N21/64

CPC classification number: G01N21/64 ,  G01J3/0235 ,  G01J3/0264 ,  G01J3/027 ,  G01J3/4406 ,  G01N21/274 ,  G01N21/278 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6458 ,  G01N2021/6471 ,  G02B21/365 ,  G06T5/009 ,  G06T5/40 ,  G06T7/74 ,  G06T7/80 ,  G06T2207/10024 ,  G06T2207/10056 ,  G06T2207/10064

Abstract: Systems and methods for standardizing one or more fluorescence scanning instruments to a reference system by separating the effects of drift and normalization. In an embodiment, a drift image comprising an image of a drift reference slide is captured by a system to be standardized. A drift measurement is calculated using the drift image. A first normalization image comprising an image of a normalization slide is also captured by the system to be standardized. A reference normalization image, also comprising an image of the normalization slide, is captured by a reference system. The first normalization image is compared to the reference normalization image to determine a gamma value and offset value for the system to be standardized.

Abstract translation: 通过分离漂移和归一化的影响将一个或多个荧光扫描仪器标准化到参考系统的系统和方法。 在一个实施例中，包括漂移参考幻灯片的图像的漂移图像由待标准化的系统捕获。 使用漂移图像计算漂移测量。 包括标准化幻灯片的图像的第一归一化图像也被要标准化的系统捕获。 还包括归一化幻灯片的图像的参考归一化图像由参考系统捕获。 将第一归一化图像与参考归一化图像进行比较，以确定要标准化的系统的伽马值和偏移值。

公开(公告)号：EP1631872A2

公开(公告)日：2006-03-08

申请号：EP04715734.2

申请日：2004-02-27

Applicant: Aperio Technologies, Inc.

Inventor： EICHHORN, Ole ,  OLSON, Allen

IPC: G06F1/00

CPC classification number: G06T7/0012 ,  G06F19/00 ,  G06F19/321 ,  G06T1/00 ,  G06T2207/10056 ,  G06T2207/20016

Abstract: A system and method for processing and analyzing virtual microscopy digital images ("virtual slides") is provided. The system comprises an algorithm server that maintains or has access to a plurality of image processing and analysis routines. The algorithm server additionally has access to a plurality of virtual slides. The algorithm server executes a selected routine on an identified virtual slide and provides the resulting data. The virtual slide can be accessed locally or remotely across a network. Similarly, the image processing routines can be obtained from local storage or across a network, or both. Advantageously, certain common sub-routines may be stored locally for inclusion in other local or remotely obtained routines. Access to image processing and analysis may be restricted through a monitor process that authenticates requests to process or view virtual slides. Variations in restrictions to images provide a rich diversity in access levels that allow sharing of virtual slides and demonstrations of image processing algorithms.

公开(公告)号：EP2510494A2

公开(公告)日：2012-10-17

申请号：EP10836742.6

申请日：2010-12-10

Applicant: Aperio Technologies, Inc.

Inventor： OLSON, Allen ,  EICHHORN, Ole

IPC: G06Q50/00 ,  G06T7/00

CPC classification number: G06T7/0012 ,  G06F19/00 ,  G06F19/321 ,  G06T2207/10056 ,  G06T2207/30024

Abstract: A digital slide analysis system comprises an algorithm server that maintains or has access to a plurality of image processing and analysis routines. The algorithm server additionally has access to a plurality of digital slide images. The algorithm server executes a selected routine on an identified digital slide and provides the resulting data. Prior to the application of selected routine, the system employs a digital pre-processing module to create a metadata mask that reduces undesirable image data such that the image data processed by the selected routine has an improved signal to noise ratio. The pre-processing module uses a classifier that may be implemented as a pattern recognition module, for example. Undesirable image data is therefore excluded from the image data that is processed by the digital pathology image processing and analysis routine, which significantly improves the digital pathology image analysis.

Abstract translation: 数字幻灯片分析系统包括维护或访问多个图像处理和分析例程的算法服务器。 算法服务器还可以访问多个数字幻灯片图像。 算法服务器在识别的数字幻灯片上执行选择的例程，并提供结果数据。 在应用选择的例程之前，系统采用数字预处理模块来创建元数据掩码，其减少不期望的图像数据，使得由所选程序处理的图像数据具有改善的信噪比。 预处理模块使用例如可以被实现为模式识别模块的分类器。 因此，不希望的图像数据被排除在由数字病理图像处理和分析程序处理的图像数据中，这显着改善了数字病理图像分析。