公开(公告)号：EP0497434A3

公开(公告)日：1992-12-16

申请号：EP92201182.0

申请日：1989-02-14

Applicant: APPLIED BIOSYSTEMS, INC.

Inventor： Sapp, Edwin R. ,  Kinast, Eric K.

IPC: G01N21/27 ,  G01J1/16 ,  G01J3/42

CPC classification number: G01N21/27 ,  G01J3/2803 ,  G01J3/42 ,  G01J2001/161 ,  G01J2001/1673 ,  G01N30/74 ,  G01N2201/1232 ,  G01N2201/126

Abstract: The present invention relates to an improved dual beam multichannel spectrophotomer employing a simple and novel optical system in combination with photodiode arrays and a unique logrithmic data converter to convert light signals to absorbance. In particular, the optical system utilizes optical elements in a novel arrangement to direct a pair of equivalent sample and reference beams in an essentially parallel formation respectively through a sample and reference cell and to focus and direct the emergent sample and reference beams to a single flat horizonatally ruled grating which disperses each of the sample and reference beams respectively onto a pair of vertically disposed photodiode arrays whereby the light signals are converted into absorbance units (AU) by an unique logarithmic data converter. The spectrophotometer is highly accurate, has very low drift, less than 2 x 10⁻⁴ AU/°C, and very low noise, less than ±2 x 10⁻⁵AU. The dual beam multichannel spectrophotomer is particularly suitable for use in high pressure liquid chromatography to record the absorbance spectrum of the samples as they are being eluted from the chromatographic column.

公开(公告)号：EP0497434B1

公开(公告)日：1996-10-23

申请号：EP92201182.0

申请日：1989-02-14

Applicant: APPLIED BIOSYSTEMS, INC.

Inventor： Sapp, Edwin R. ,  Kinast, Eric K.

IPC: G01N21/27 ,  G01J1/16 ,  G01J3/42

CPC classification number: G01N21/27 ,  G01J3/2803 ,  G01J3/42 ,  G01J2001/161 ,  G01J2001/1673 ,  G01N30/74 ,  G01N2201/1232 ,  G01N2201/126

公开(公告)号：EP0329061A3

公开(公告)日：1991-11-13

申请号：EP89102478.8

申请日：1989-02-14

Applicant: APPLIED BIOSYSTEMS, INC.

Inventor： Sapp, Edwin R. ,  Kinast, Eric K.

IPC: G01N21/27 ,  G01J1/16 ,  G01J3/42

CPC classification number: G01N21/27 ,  G01J3/2803 ,  G01J3/42 ,  G01J2001/161 ,  G01J2001/1673 ,  G01N30/74 ,  G01N2201/1232 ,  G01N2201/126

Abstract: The present invention relates to an improved dual beam multichannel spectrophotomer employing a simple and novel optical system in combination with photodiode arrays and a unique logrithmic data converter to convert light signals to absorbance. In particular, the optical system utilizes optical elements in a novel arrangement to direct a pair of equivalent sample and reference beams in an essentially parallel formation respectively through a sample and reference cell and to focus and direct the emergent sample and reference beams to a single flat horizontally ruled grating which disperses each of the sample and reference beams respectively onto a pair of vertically disposed photodiode arrays whereby the light signals are converted into absorbance units (AU) by an unique logarithmic data converter. The spectrophotometer is highly accurate, has very low drift, less than 2 x 10⁻⁴ AU/°C, and very low noise, less than ±2 x 10⁻⁵AU. The dual beam multichannel spectrophotomer is particularly suitable for use in high pressure liquid chromatography to record the absorbance spectrum of the samples as they are being eluted from the chromatographic column.

公开(公告)号：EP0497434A2

公开(公告)日：1992-08-05

申请号：EP92201182.0

申请日：1989-02-14

Applicant: APPLIED BIOSYSTEMS, INC.

Inventor： Sapp, Edwin R. ,  Kinast, Eric K.

IPC: G01N21/27 ,  G01J1/16 ,  G01J3/42

CPC classification number: G01N21/27 ,  G01J3/2803 ,  G01J3/42 ,  G01J2001/161 ,  G01J2001/1673 ,  G01N30/74 ,  G01N2201/1232 ,  G01N2201/126

Abstract: The present invention relates to an improved dual beam multichannel spectrophotomer employing a simple and novel optical system in combination with photodiode arrays and a unique logrithmic data converter to convert light signals to absorbance. In particular, the optical system utilizes optical elements in a novel arrangement to direct a pair of equivalent sample and reference beams in an essentially parallel formation respectively through a sample and reference cell and to focus and direct the emergent sample and reference beams to a single flat horizonatally ruled grating which disperses each of the sample and reference beams respectively onto a pair of vertically disposed photodiode arrays whereby the light signals are converted into absorbance units (AU) by an unique logarithmic data converter. The spectrophotometer is highly accurate, has very low drift, less than 2 x 10⁻⁴ AU/°C, and very low noise, less than ±2 x 10⁻⁵AU. The dual beam multichannel spectrophotomer is particularly suitable for use in high pressure liquid chromatography to record the absorbance spectrum of the samples as they are being eluted from the chromatographic column.

Abstract translation: 本发明涉及一种改进的双光束多通道分光光度计，其采用与光电二极管阵列组合的简单且新颖的光学系统以及将光信号转换成吸光度的唯一的对数数据转换器。 特别地，光学系统利用新颖的布置中的光学元件来分别通过样品和参考单元引导基本上平行的一对等效样品和参考光束，并将出射样品和参考光束聚焦并引导到单个平面 分别将每个样品和参考光束分散在一对垂直设置的光电二极管阵列上，由此光信号通过独特的对数数据转换器转换成吸光度单位（AU）。 分光光度计是高精度的，具有非常低的漂移，小于2×10 -4 AU /℃，噪音非常低，小于+/- 2×10 <5> AU。 双光束多通道分光光度计特别适用于高压液相色谱，以记录样品从色谱柱中洗脱的吸收光谱。

公开(公告)号：EP0329061A2

公开(公告)日：1989-08-23

申请号：EP89102478.8

申请日：1989-02-14

Applicant: APPLIED BIOSYSTEMS, INC.

Inventor： Sapp, Edwin R. ,  Kinast, Eric K.

IPC: G01N21/27 ,  G01J1/16 ,  G01J3/42

CPC classification number: G01N21/27 ,  G01J3/2803 ,  G01J3/42 ,  G01J2001/161 ,  G01J2001/1673 ,  G01N30/74 ,  G01N2201/1232 ,  G01N2201/126

Abstract: The present invention relates to an improved dual beam multichannel spectrophotomer employing a simple and novel optical system in combination with photodiode arrays and a unique logrithmic data converter to convert light signals to absorbance. In particular, the optical system utilizes optical elements in a novel arrangement to direct a pair of equivalent sample and reference beams in an essentially parallel formation respectively through a sample and reference cell and to focus and direct the emergent sample and reference beams to a single flat horizontally ruled grating which disperses each of the sample and reference beams respectively onto a pair of vertically disposed photodiode arrays whereby the light signals are converted into absorbance units (AU) by an unique logarithmic data converter. The spectrophotometer is highly accurate, has very low drift, less than 2 x 10⁻⁴ AU/°C, and very low noise, less than ±2 x 10⁻⁵AU. The dual beam multichannel spectrophotomer is particularly suitable for use in high pressure liquid chromatography to record the absorbance spectrum of the samples as they are being eluted from the chromatographic column.

Abstract translation: 本发明涉及一种改进的双光束多通道分光光度计，其采用与光电二极管阵列组合的简单且新颖的光学系统以及将光信号转换成吸光度的唯一的对数数据转换器。 特别地，光学系统利用新颖的布置中的光学元件来分别通过样品和参考单元引导基本上平行的一对等效样品和参考光束，并将出射样品和参考光束聚焦并引导到单个平面 将每个样本和参考光束分别分散在一对垂直设置的光电二极管阵列上的水平格栅光栅，由此光信号通过独特的对数数据转换器转换成吸光度单位（AU）。 分光光度计是高精度的，具有非常低的漂移，小于2×10 -4 AU /℃，噪音非常低，小于+/- 2×10 <5> AU。 双光束多通道分光光度计特别适用于高压液相色谱，以记录样品从色谱柱中洗脱的吸收光谱。