公开(公告)号：WO2007080583A3

公开(公告)日：2009-02-12

申请号：PCT/IL2007000034

申请日：2007-01-09

Applicant: APPLIED SPECTRAL IMAGING LTD ,  KATZIR NIR ,  KOLATT TSAFRIR ,  BAR-AM IRIT

Inventor： KATZIR NIR ,  KOLATT TSAFRIR ,  BAR-AM IRIT

IPC: G01N33/48 ,  C12Q1/68

CPC classification number: C12Q1/6841 ,  G06K9/00134

Abstract: Methods, computer readable storage media and systems which can be used for analyzing labeled biological samples, identifying chromosomal aberrations, identifying genetically abnormal cells and/or computationally scanning the samples using randomly or randomized scanning methods are provided. Specifically, the present invention can be used to analyze FISH-stained samples and automatically identify chromosomal aberrations associated with abnormal intensity ratio of stained occurrences in the sample.

Abstract translation: 提供了可用于分析标记的生物样品，识别染色体畸变，识别遗传异常细胞和/或使用随机或随机扫描方法计算扫描样品的方法，计算机可读存储介质和系统。 具体地，本发明可用于分析FISH染色的样品，并自动识别与样品中染色事件的异常强度比相关的染色体畸变。

公开(公告)号：AU5123599A

公开(公告)日：2000-02-21

申请号：AU5123599

申请日：1999-07-23

Applicant: APPLIED SPECTRAL IMAGING LTD

Inventor： BAR-AM IRIT ,  JANISH CATHY ,  GARINI YUVAL ,  CABIB DARIO ,  BUCKWALD ROBERT A

IPC: G01N33/53 ,  C12N15/09 ,  C12Q1/68 ,  C12Q1/6841 ,  G01N33/533 ,  G01N33/566 ,  C07H21/04

Abstract: A fluorescent in situ hybridization method including the steps of (a) obtaining a chromosome spread of a species; (b) preparing a hybridization composite containing a plurality of chromosomal paints each of the plurality of chromosomal paints being labeled with a different fluorophore-or-combination-of-fluorophores, such that an averaged specific activity of highly repetitive sequences in the hybridization composite substantially equals an averaged specific activity of unique sequences in the hybridization composite; (c) denaturing the hybridization composite and subjecting the hybridization composite to conditions for allowing at least a part of the highly repetitive sequences in the hybridization composite to reanneal while at least a part of the unique sequences in the hybridization composite remaining single stranded; (d) contacting under hybridization conditions the hybridization composite with the chromosome spread; (e) washing away excess of the hybridization composite; and (d) analyzing and presenting images of the now hybridized chromosome spread.

公开(公告)号：EP1100965A4

公开(公告)日：2004-11-03

申请号：EP99935850

申请日：1999-07-23

Applicant: APPLIED SPECTRAL IMAGING LTD

Inventor： BAR-AM IRIT ,  JANISH CATHY ,  GARINI YUVAL ,  CABIB DARIO ,  BUCKWALD ROBERT A

IPC: G01N33/53 ,  C12N15/09 ,  C12Q1/68 ,  G01N33/533 ,  G01N33/566 ,  C07H21/04

CPC classification number: C12Q1/6841 ,  C12Q2527/125 ,  C12Q2563/107 ,  C12Q2527/101 ,  C12Q2525/151

Abstract: A fluorescent in situ hybridization method including the steps of (a) obtaining a chromosome spread of a species; (b) preparing a hybridization composite containing a plurality of chromosomal paints each of the plurality of chromosomal paints being labeled with a different fluorophore-or-combination-of-fluorophores, such that an averaged specific activity of highly repetitive sequences in the hybridization composite substantially equals an averaged specific activity of unique sequences in the hybridization composite; (c) denaturing the hybridization composite and subjecting the hybridization composite to conditions for allowing at least a part of the highly repetitive sequences in the hybridization composite to reanneal while at least a part of the unique sequences in the hybridization composite remaining single stranded; (d) contacting under hybridization conditions the hybridization composite with the chromosome spread; (e) washing away excess of the hybridization composite; and (f) analyzing and presenting images of the now hybridized chromosome spread.