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    METHOD FOR GENERATION OF LONGER cDNA FRAGMENTS FROM SAGE TAGS FOR GENE IDENTIFICATION
    2.
    发明申请
    METHOD FOR GENERATION OF LONGER cDNA FRAGMENTS FROM SAGE TAGS FOR GENE IDENTIFICATION 审中-公开
    用于从用于基因鉴定的标签生成更长的cDNA片段的方法

    公开(公告)号：WO0148247A3

    公开(公告)日：2002-09-06

    申请号：PCT/US0035579

    申请日：2000-12-22

    Applicant: ARCH DEV CORP

    Inventor： WANG SAN MING ,  CHEN JIANJUN ,  ROWLEY JANET D

    IPC: C07H21/02 ,  C07H21/04 ,  C12N15/10 ,  C12P19/34 ,  C12Q1/68

    CPC classification number: C12Q1/6853 ,  C12N15/1096

    Abstract: Generation of longer cDNA fragments from SAGE tags for gene identification (GLGI) is disclosed. This method converts SAGE tags, which are about 10 base pairs in length, into their corresponding 3'cDNA fragments covering hundred bases. This added information provides for more accurate genome-wide analysis and overcomes the inherent deficiencies of SAGE. The generation of longer cDNA fragments from isolated and purified protein fragments for gene identification is also disclosed. This method converts a short amino acid sequence into extended version of the DNA sequences encoding the protein/protein fragment and additional 3' end sequences of the gene encoding the protein. This additional sequence information allows gene identification from purified protein sequences. The invention also provides a high-throughput GLGI procedure for identifying genes corresponding to a set of unidentified SAGE tags.

    Abstract translation: 公开了用于基因鉴定（GLGI）的SAGE标签的较长cDNA片段的产生。 该方法将长度约为10个碱基对的SAGE标签转换成覆盖百个碱基的相应的3'cDNA片段。 这个增加的信息提供了更准确的全基因组分析，并克服了SAGE的固有缺陷。 还公开了用于基因鉴定的分离和纯化的蛋白质片段产生更长的cDNA片段。 该方法将短氨基酸序列转化为编码蛋白质/蛋白质片段的DNA序列和编码蛋白质的基因的另外的3'末端序列的扩增形式。 这个额外的序列信息允许从纯化的蛋白质序列中进行基因鉴定。 本发明还提供了用于鉴定与一组不明身份的SAGE标签相对应的基因的高通量GLGI程序。

    Method for generation of longer cdna fragments from sage tags for gene identification
    3.
    发明专利
    Method for generation of longer cdna fragments from sage tags for gene identification 未知

    公开(公告)号：AU2743001A

    公开(公告)日：2001-07-09

    申请号：AU2743001

    申请日：2000-12-22

    Applicant: ARCH DEV CORP

    Inventor： WANG SAN MING ,  CHEN JIAN-JUN ,  ROWLEY JANET D

    IPC: C07H21/02 ,  C07H21/04 ,  C12N15/10 ,  C12P19/34 ,  C12Q1/68

    Abstract: Generation of longer cDNA fragments from SAGE tags for gene identification (GLGI) is disclosed. This method converts SAGE tags, which are about 10 base pairs in length, into their corresponding 3' cDNA fragments covering hundred bases. This added information provides for more accurate genome-wide analysis and overcomes the inherent deficiencies of SAGE. The generation of longer cDNA fragments from isolated and purified protein fragments for gene identification is also disclosed. This method converts a short amino acid sequence into extended versions of the DNA sequences encoding the protein/protein fragment and additional 3' end sequences of the gene encoding the protein. This additional sequence information allows gene identification from purified protein sequences. The invention also provides a high-throughput GLGI procedure for identifying genes corresponding to a set of unidentified SAGE tags.

    METHOD FOR GENERATION OF LONGER CDNA FRAGMENTS FROM SAGE TAGS FOR GENE IDENTIFICATION
    4.
    发明专利
    METHOD FOR GENERATION OF LONGER CDNA FRAGMENTS FROM SAGE TAGS FOR GENE IDENTIFICATION 未知

    公开(公告)号：CA2395920A1

    公开(公告)日：2001-07-05

    申请号：CA2395920

    申请日：2000-12-22

    Applicant: ARCH DEV CORP

    Inventor： CHEN JIANJUN ,  WANG SAN MING ,  ROWLEY JANET D

    IPC: C07H21/02 ,  C07H21/04 ,  C12N15/10 ,  C12P19/34 ,  C12Q1/68

    Abstract: Generation of longer cDNA fragments from SAGE tags for gene identification (GLGI) is disclosed. This method converts SAGE tags, which are about 10 base pairs in length, into their corresponding 3'cDNA fragments covering hundred bases. This added information provides for more accurate genome-wide analysi s and overcomes the inherent deficiencies of SAGE. The generation of longer cD NA fragments from isolated and purified protein fragments for gene identificati on is also disclosed. This method converts a short amino acid sequence into extended version of the DNA sequences encoding the protein/protein fragment and additional 3' end sequences of the gene encoding the protein. This additional sequence information allows gene identification from purified protein sequences. The invention also provides a high-throughput GLGI procedure for identifying genes corresponding to a set of unidentified SAGE tags.

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