公开(公告)号：WO2007080583A2

公开(公告)日：2007-07-19

申请号：PCT/IL2007/000034

申请日：2007-01-09

Applicant: APPLIED SPECTRAL IMAGING LTD. ,  KATZIR, Nir ,  KOLATT, Tsafrir ,  BAR-AM, Irit

Inventor： KATZIR, Nir ,  KOLATT, Tsafrir ,  BAR-AM, Irit

IPC: C12Q1/68 ,  G06F19/00

CPC classification number: C12Q1/6841 ,  G06K9/00134

Abstract: Methods, computer readable storage media and systems which can be used for analyzing labeled biological samples, identifying chromosomal aberrations, identifying genetically abnormal cells and/or computationally scanning the samples using randomly or randomized scanning methods are provided. Specifically, the present invention can be used to analyze FISH-stained samples and automatically identify chromosomal aberrations associated with abnormal intensity ratio of stained occurrences in the sample.

Abstract translation: 提供了可用于分析标记的生物样品，识别染色体畸变，识别遗传异常细胞和/或使用随机或随机扫描方法计算扫描样品的方法，计算机可读存储介质和系统。 具体地，本发明可用于分析FISH染色的样品，并自动识别与样品中染色事件的异常强度比相关的染色体畸变。

公开(公告)号：EP1100965A1

公开(公告)日：2001-05-23

申请号：EP99935850.0

申请日：1999-07-23

Applicant: Applied Spectral Imaging Ltd.

Inventor： BAR-AM, Irit ,  JANISH, Cathy ,  GARINI, Yuval ,  CABIB, Dario ,  BUCKWALD, Robert, A.

IPC: C12Q1/68 ,  C07H21/04

CPC classification number: C12Q1/6841 ,  C12Q2527/125 ,  C12Q2563/107 ,  C12Q2527/101 ,  C12Q2525/151

Abstract: A fluorescent in situ hybridization method including the steps of (a) obtaining a chromosome spread of a species; (b) preparing a hybridization composite containing a plurality of chromosomal paints each of the plurality of chromosomal paints being labeled with a different fluorophore-or-combination-of-fluorophores, such that an averaged specific activity of highly repetitive sequences in the hybridization composite substantially equals an averaged specific activity of unique sequences in the hybridization composite; (c) denaturing the hybridization composite and subjecting the hybridization composite to conditions for allowing at least a part of the highly repetitive sequences in the hybridization composite to reanneal while at least a part of the unique sequences in the hybridization composite remaining single stranded; (d) contacting under hybridization conditions the hybridization composite with the chromosome spread; (e) washing away excess of the hybridization composite; and (f) analyzing and presenting images of the now hybridized chromosome spread.