公开(公告)号：JPH11243975A

公开(公告)日：1999-09-14

申请号：JP36575198

申请日：1998-12-22

Applicant: BASF AG

Inventor： POMPEJUS MARKUS ,  SEULBERGER HARALD ,  HOFFKEN HANS WOLFGANG ,  DOVAL JOSE LUIS REVUELTA ,  JIMENEZ ALBERTO ,  GARCIA MARIA ANGELES SANTOS

IPC: C12N15/09 ,  C07K14/39 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/00 ,  C12N9/02 ,  C12N9/04 ,  C12N9/10 ,  C12N9/12 ,  C12N15/00 ,  C12N15/52 ,  C12N15/53 ,  C12N15/80 ,  C12P19/28 ,  C12P25/00 ,  C12R1/645

Abstract: PROBLEM TO BE SOLVED: To obtain a new protein capable of carrying out the riboflavin biosynthesis by using a gene of an enzyme for the biosysnthesis of purine from Ashbya gossipii having a specific polypeptide sequence and the enzyme activity of a phosphoribosyl pyrophosphate synthase. SOLUTION: This new protein has a polypeptide sequence represented by the formula or a polypeptide sequence obtained by substituting, inserting or deleting up to 15% amino acids from the formula and the enzyme activity of a phosphoribosyl pyrophosphate synthase and is capable of carrying out the biosynthesis of riboflavin (vitamin B2 ) by expressing a gene from an enzyme for biosynthesis of purine from Ashbya gossipii in a microorganism. The protein is obtained by extracting a genomic DNA from Ashbya gossipii ATCC10895, then producing a genomic genetic bank according to a conventional method, cloning the resultant bank, providing a gene of an enzyme for the biosynthesis of the purine and expressing the resultant gene in the microorganism according to a gene technical constitution of the microorganism. The prepared protein is used to perform the biosynthesis of the riboflavin in the microorganism.