公开(公告)号：JPH11243975A

公开(公告)日：1999-09-14

申请号：JP36575198

申请日：1998-12-22

Applicant: BASF AG

Inventor： POMPEJUS MARKUS ,  SEULBERGER HARALD ,  HOFFKEN HANS WOLFGANG ,  DOVAL JOSE LUIS REVUELTA ,  JIMENEZ ALBERTO ,  GARCIA MARIA ANGELES SANTOS

IPC: C12N15/09 ,  C07K14/39 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/00 ,  C12N9/02 ,  C12N9/04 ,  C12N9/10 ,  C12N9/12 ,  C12N15/00 ,  C12N15/52 ,  C12N15/53 ,  C12N15/80 ,  C12P19/28 ,  C12P25/00 ,  C12R1/645

Abstract: PROBLEM TO BE SOLVED: To obtain a new protein capable of carrying out the riboflavin biosynthesis by using a gene of an enzyme for the biosysnthesis of purine from Ashbya gossipii having a specific polypeptide sequence and the enzyme activity of a phosphoribosyl pyrophosphate synthase. SOLUTION: This new protein has a polypeptide sequence represented by the formula or a polypeptide sequence obtained by substituting, inserting or deleting up to 15% amino acids from the formula and the enzyme activity of a phosphoribosyl pyrophosphate synthase and is capable of carrying out the biosynthesis of riboflavin (vitamin B2 ) by expressing a gene from an enzyme for biosynthesis of purine from Ashbya gossipii in a microorganism. The protein is obtained by extracting a genomic DNA from Ashbya gossipii ATCC10895, then producing a genomic genetic bank according to a conventional method, cloning the resultant bank, providing a gene of an enzyme for the biosynthesis of the purine and expressing the resultant gene in the microorganism according to a gene technical constitution of the microorganism. The prepared protein is used to perform the biosynthesis of the riboflavin in the microorganism.

公开(公告)号：WO9961623A3

公开(公告)日：2000-01-27

申请号：PCT/EP9903196

申请日：1999-05-10

Applicant: BASF AG ,  ALTHOEFER HENNING ,  SEULBERGER HARALD ,  ZELDER OSKAR ,  REVUELTA DOVAL JOSE LUIS

Inventor： ALTHOEFER HENNING ,  SEULBERGER HARALD ,  ZELDER OSKAR ,  REVUELTA DOVAL JOSE LUIS

IPC: C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N15/54 ,  C12N15/80 ,  C12P25/00 ,  C12N15/52

CPC classification number: C12P25/00 ,  C12N15/80

Abstract: The invention relates to a genetic method for producing riboflavin. The production of riboflavin in organisms is increased by specially selecting genes of the riboflavin biosynthesis or of the combination thereof in organisms, especially in bacteria, fungi, yeasts and plants, and of the expression thereof. The invention also relates to a nucleic acid fragment containing genes with the sequences SEQ ID No. 1, SEQ ID No. 3 or SEQ ID No. 5 or the functional equivalents thereof, to expression vectors containing the nucleic acid fragments, and to organisms containing at least one nucleic acid fragment or at least one vector.

Abstract translation: 本发明涉及一种用于核黄素的制备遗传程序。 由核黄素生物合成或在生物体它们的组合，特别是在细菌，真菌，酵母，植物和它们的表达的基因的特殊选择，核黄素产量在这些生物中增加。 本发明还涉及核酸片段具有序列SEQ ID号含有基因 1，SEQ ID号 3或SEQ ID号 其5个或功能等同物，包括含有至少一种核酸片段或至少一种载体中的核酸片段和有机体的表达载体。

公开(公告)号：WO9829538A3

公开(公告)日：1998-12-10

申请号：PCT/EP9707309

申请日：1997-12-29

Applicant: BASF AG ,  ALTMANN JOEHL REGULA ,  PHILIPPSEN PETER ,  ALTHOEFER HENNING ,  SEULBERGER HARALD

Inventor： ALTMANN-JOEHL REGULA ,  PHILIPPSEN PETER ,  ALTHOEFER HENNING ,  SEULBERGER HARALD

IPC: C12N15/09 ,  C07K14/37 ,  C12N1/15 ,  C12N9/02 ,  C12N9/04 ,  C12N9/10 ,  C12N9/88 ,  C12N15/00 ,  C12N15/31 ,  C12N15/60 ,  C12P25/00 ,  C12Q1/18 ,  C12Q1/68 ,  C12R1/645 ,  C12R1/865

CPC classification number: C07K14/37 ,  C12N9/0006 ,  C12N9/1077 ,  C12N9/88 ,  C12P25/00

Abstract: A gene is disclosed which contains the nucleotide sequence represented by SE ID NO:1 and which extends from nucleotide 671 to nucleotide 6295, or a nucleotide sequence derived therefrom by substitution, insertion or deletion of up to 30 %, 20 %, 10 % or 5 % of its nucleotides, the lower percentages being preferable, and whose genetic product exhibits the enzymatic activity of an adenylate cyclase. Also disclosed is its use.

公开(公告)号：WO9829539A3

公开(公告)日：1998-11-12

申请号：PCT/EP9707312

申请日：1997-12-29

Applicant: BASF AG ,  PHILIPPSEN PETER ,  POMPEJUS MARKUS ,  SEULBERGER HARALD

Inventor： PHILIPPSEN PETER ,  POMPEJUS MARKUS ,  SEULBERGER HARALD

IPC: C12N15/09 ,  C07K14/37 ,  C12N1/15 ,  C12N9/02 ,  C12N9/04 ,  C12N9/10 ,  C12N9/88 ,  C12N15/00 ,  C12N15/31 ,  C12N15/60 ,  C12P25/00 ,  C12Q1/18 ,  C12Q1/68 ,  C12R1/645 ,  C12R1/865

CPC classification number: C07K14/37 ,  C12N9/0006 ,  C12N9/1077 ,  C12N9/88 ,  C12P25/00

Abstract: Partial sequences of purine biosynthesis genes from Ashbya gossypii are disclosed, as well as their production and use.

公开(公告)号：ES2232035T3

公开(公告)日：2005-05-16

申请号：ES98966671

申请日：1998-12-23

Applicant: BASF AG

Inventor： REVUELTA DOVAL JOSE LUIS ,  SANTOS GARCIA MARIA ANGELES ,  POMPEJUS MARKUS ,  SEULBERGER HARALD

IPC: C12N15/09 ,  C07K14/39 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/00 ,  C12N9/02 ,  C12N9/04 ,  C12N9/10 ,  C12N9/12 ,  C12N15/00 ,  C12N15/52 ,  C12N15/53 ,  C12N15/80 ,  C12P19/28 ,  C12P25/00 ,  C12R1/645

Abstract: Secuencia de ADN adecuada como promotor, que contiene la estructura primaria representada en SEQ ID NO: 1 Posiciones 9 a 307.

公开(公告)号：ZA986188B

公开(公告)日：2000-01-13

申请号：ZA986188

申请日：1998-07-13

Applicant: BASF AG

Inventor： SEULBERGER HARALD ,  LERCHL JENS ,  SCHMIDT RALF-MICHAEL ,  KRUPINSKA KARIN ,  FALK JOH

IPC: C12N9/02 ,  C12N15/53 ,  C12N15/82 ,  C12N ,  A01H ,  C07H

公开(公告)号：AU8216998A

公开(公告)日：1999-02-10

申请号：AU8216998

申请日：1998-06-23

Applicant: BASF AG

Inventor： SEULBERGER HARALD ,  LERCHL JENS ,  SCHMIDT RALF-MICHAEL ,  KRUPINSKA KARIN ,  FALK JON

IPC: C12N9/02 ,  C12N15/53 ,  C12N15/82 ,  A01H5/00 ,  C12N5/10 ,  G01N33/50

公开(公告)号：AU6291698A

公开(公告)日：1998-07-31

申请号：AU6291698

申请日：1997-12-29

Applicant: BASF AG

Inventor： ALTMANN-JOHL REGULA ,  PHILIPPSEN PETER ,  ALTHOFER HENNING ,  SEULBERGER HARALD

IPC: C12N15/09 ,  C07K14/37 ,  C12N1/15 ,  C12N9/02 ,  C12N9/04 ,  C12N9/10 ,  C12N9/88 ,  C12N15/00 ,  C12N15/31 ,  C12N15/60 ,  C12P25/00 ,  C12Q1/18 ,  C12Q1/68 ,  C12R1/645 ,  C12R1/865

Abstract: The present invention relates to the terminal sequencing of random genomic fragments performed with the filamentous fungus A.gossypii, to the sequences obtained therewith and the use of the sequences for forensic identification, to characterize genes and gene organization of this ascomycete by inter-genomic comparison, to identify biosynthetic genes that can be used as selection markers, to isolate promotors and terminators for application in a homologous as well as heterologous context, to find putative centromere containing clones, chromosome mapping, chromosome identifying, general information about chromosome organization and in addition to identify ORF containing SRS sequences with no homology to S. cerevisiae or any other organism which allows the identification of A. gossypii specific genes.

公开(公告)号：DE19545468A1

公开(公告)日：1997-08-21

申请号：DE19545468

申请日：1995-12-06

Applicant: FORSCHUNGSZENTRUM JUELICH GMBH ,  BASF AG

Inventor： STAHMANN KLAUS-PETER ,  BOEDDECKER THEO ,  SAHM HERMANN ,  SEULBERGER HARALD

IPC: C07D475/14 ,  C12N1/15 ,  C12N15/60 ,  C12P25/00 ,  C12N15/63 ,  C12N1/00 ,  C12N15/80

Abstract: In the microbial prodn. of riboflavin (Rf) by growing a Rf-producing microorganism in a nutrient medium, the new feature is that the endogenous isocitrate lyase (ICL) activity of the microorganism has been altered. Also new are: (1) ICL gene encoding the 560 residue amino acid sequence (I), given in the specification; and (2) genetic construct contg. the ICL gene.

公开(公告)号：NO962023D0

公开(公告)日：1996-05-15

申请号：NO962023

申请日：1996-05-15

Applicant: BASF AG

Inventor： KROGER BURKHARD ,  SEULBERGER HARALD ,  MEYER THOMAS ,  SCHMIDT MARTIN ,  JACOB ELARD ,  OTTER RAINER ,  SUBKOWSKI THOMAS ,  HILLEN HEINZ

IPC: G01N33/573 ,  A01K67/027 ,  A61K31/70 ,  A61K35/12 ,  A61K38/00 ,  A61K38/43 ,  A61K38/48 ,  A61K39/395 ,  A61K48/00 ,  A61P9/00 ,  A61P9/08 ,  A61P9/10 ,  A61P9/12 ,  A61P11/00 ,  A61P13/02 ,  A61P15/00 ,  A61P25/04 ,  A61P25/28 ,  A61P29/00 ,  A61P31/04 ,  C07H21/04 ,  C07K14/47 ,  C07K16/40 ,  C12N5/00 ,  C12N5/10 ,  C12N9/64 ,  C12N15/09 ,  C12N15/113 ,  C12N15/57 ,  C12R1/91 ,  C12N

Abstract: PCT No. PCT/EP94/03706 Sec. 371 Date May 16, 1996 Sec. 102(e) Date May 16, 1996 PCT Filed Nov. 10, 1994 PCT Pub. No. WO95/14095 PCT Pub. Date May 26, 1995The invention relates to endothelin-converting enzymes containing the polypeptide sequence described in SEQ. ID. NO. 30 or SEQ. ID. NO. 36 or functional fragments thereof, genes which code for such enzymes, and processes for producing the said enzymes and the use thereof.