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公开(公告)号：CA2428372A1
公开(公告)日：2002-05-23
申请号：CA2428372
申请日：2001-11-14
Applicant: BASF AG , WEBER GMBH DR
Inventor： PLATSCH HERBERT , WEBER GERHARD , HAMMERMANN MARKUS , EIPEL HEINZ
IPC: G01N27/447 , G01N33/68
Abstract: The invention relates to a method for separating and detecting proteins or protein samples of cellular origin. Said proteins are contained in a separation buffer solution and the following steps are carried out: the protein samples are separated into individual fractions in a first separating step by means of free flow electrophoresis, isoelectric focusing (IEF) or isotachophoresis, and are linked with a marker; the protein fractions are separated into at least one capillary by means of capillary electrophoresis in a second separating step; and at least one marker linked with the protein fractions is detected in the at least one capillary.
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公开(公告)号：DE10056838A1
公开(公告)日：2002-05-23
申请号：DE10056838
申请日：2000-11-16
Applicant: BASF AG
Inventor： EIPEL HEINZ , HAMMERMANN MARKUS , PLATSCH HERBERT , WEBER GERHARD
IPC: G01N27/447 , G01N33/68 , C07K1/26
Abstract: Separating and detecting proteins or protein samples (A) of cellular origin by subjecting them, dissolved in buffer solution, to separation by free-flow electrophoresis, isoelectric focusing or isotachophoresis into individual fractions, which are labeled, and further separation by capillary electrophoresis (CE), using capillaries, with detection of at least one label, is new.
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公开(公告)号：DE3210757A1
公开(公告)日：1983-10-06
申请号：DE3210757
申请日：1982-03-24
Applicant: BASF AG
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Patent Agency Ranking
公开(公告)号：DE3202064A1
公开(公告)日：1983-08-04
申请号：DE3202064
申请日：1982-01-23
Applicant: BASF AG
Inventor： BOUQUET ROLF , WEBER GERHARD , KOOB KNUT DIPL CHEM DR
Abstract: 1. A wet sieving apparatus for particle size analysis, comprising a plurality of superposed sieves (3) whose apertures sizes decrease progressively downards, a device (2) for supplying onto the sieves the pulverulent material which is suspended in a liquid and whose particle size distribution is to be determined with the aid of the sieves, and a collecting vessel (21), having overflow means (22), for the liquid which has run through the sieves, a distributor (5) being allocated to each sieve (3) for the purpose of supplying a conveying liquid onto it, wherein, in order to uniformly supply the sieves with the conveying liquid, the distributor is a tube (7) which is rotatably mounted at the middle on a distributor head (8), and is provided with a large mumber of radial holes (6) which are arranged in such a way that the thrust produced by the conveying liquid leaving the said holes can be utilized to rotate the said tube, and the distributors are connected to a controllable source for supplying the liquid under pressure.
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公开(公告)号：DE10135497A1
公开(公告)日：2003-01-30
申请号：DE10135497
申请日：2001-07-20
Applicant: BASF AG
Inventor： EIPEL HEINZ , HAMMERMANN MARKUS , PLATSCH HERBERT , WEBER GERHARD
IPC: G01N27/447 , G01N33/68 , C07K1/28 , G01N33/48
Abstract: Separating and detecting proteins or protein samples (A) of cellular origin by subjecting them, dissolved in buffer solution, to separation by free-flow electrophoresis, isoelectric focusing or isotachophoresis into individual fractions, which are labeled, and further separation by capillary electrophoresis (CE), using capillaries, with detection of at least one label, is new.
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公开(公告)号：AU1702902A
公开(公告)日：2002-05-27
申请号：AU1702902
申请日：2001-11-14
Applicant: BASF AG , WEBER GMBH DR
Inventor： HAMMERMANN MARKUS , PLATSCH HERBERT , WEBER GERHARD
IPC: G01N27/447 , G01N33/68
Abstract: The invention relates to a method for fractionating and detecting proteins or protein samples of cellular origin, the proteins being contained in a separation buffer solution and the following process steps being performed: the protein samples are split into individual fractions in a first separation step in accordance with free-flow electrophoresis or isoelectric focusing (IEF) or isotachophoresis and are linked to a label, in a second separation step, the protein fractions are fractionated in one or more capillaries in accordance with capillary electrophoresis, and at least one label, linked to the protein fractions, is detected in said one capillary or said several capillaries.
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公开(公告)号：DE3269759D1
公开(公告)日：1986-04-10
申请号：DE3269759
申请日：1982-12-23
Applicant: BASF AG
Inventor： BOUQUET ROLF , WEBER GERHARD , KOOB KNUT DR
Abstract: 1. A wet sieving apparatus for particle size analysis, comprising a plurality of superposed sieves (3) whose apertures sizes decrease progressively downards, a device (2) for supplying onto the sieves the pulverulent material which is suspended in a liquid and whose particle size distribution is to be determined with the aid of the sieves, and a collecting vessel (21), having overflow means (22), for the liquid which has run through the sieves, a distributor (5) being allocated to each sieve (3) for the purpose of supplying a conveying liquid onto it, wherein, in order to uniformly supply the sieves with the conveying liquid, the distributor is a tube (7) which is rotatably mounted at the middle on a distributor head (8), and is provided with a large mumber of radial holes (6) which are arranged in such a way that the thrust produced by the conveying liquid leaving the said holes can be utilized to rotate the said tube, and the distributors are connected to a controllable source for supplying the liquid under pressure.
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公开(公告)号：DE10120803A1
公开(公告)日：2002-10-31
申请号：DE10120803
申请日：2001-04-27
Applicant: BASF AG
Inventor： EIPEL HEINZ , HAMMERMANN MARKUS , PLATSCH HERBERT , WEBER GERHARD
IPC: G01N27/447 , G01N33/68 , C07K1/26 , C07K1/28 , G01N33/48
Abstract: Separating and detecting proteins or protein samples (A) of cellular origin by subjecting them, dissolved in buffer solution, to separation by free-flow electrophoresis, isoelectric focusing or isotachophoresis into individual fractions, which are labeled, and further separation by capillary electrophoresis (CE), using capillaries, with detection of at least one label, is new.
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