公开(公告)号：WO2011009849A3

公开(公告)日：2011-04-07

申请号：PCT/EP2010060458

申请日：2010-07-20

Applicant: BASF SE ,  MAX PLANCK GESELLSCHAFT ,  STUERMER RAINER ,  SCHNEIDER NINA ,  BOY MATTHIAS ,  ACHATZ BRIGITTE ,  RABUS RALF ,  HEIDER JOHANN

Inventor： STUERMER RAINER ,  SCHNEIDER NINA ,  BOY MATTHIAS ,  ACHATZ BRIGITTE ,  RABUS RALF ,  HEIDER JOHANN

IPC: C12P7/62 ,  C12N9/04 ,  C12P7/64

CPC classification number: C12P7/62 ,  C12N9/0008 ,  C12P7/6436

Abstract: The invention relates to a method for preparing optically active 2-hydroxy acid ester derivatives of the formula R1-C(OH)-C(O)-O-R2 (I),comprising the bringing into contact of 2-oxo acid ester derivatives of the formula R1-C(O)-C(O)-O-R2 (II) with an enzyme (E) selected from the class of dehydrogenases, in the presence of reduction equivalents, where the compound of the formula (II) is enzymatically reduced to the compound of the formula (I), and the reduction equivalents consumed in the course of the reaction are regenerated again by converting a reducing agent (RA) to the corresponding oxidation product (OP) with the help of the enzyme (E). The invention further comprises a dehydrogenase which reduces 2-oxo acid esters in the presence of reduction equivalents to the corresponding S- 2-hydroxy acid esters, and also a nucleic acid encoding the dehydrogenase.

Abstract translation: 本发明涉及制备式R 1 -C（OH）-C（O）-O-R 2（I）的光学活性2-羟基酸酯衍生物的方法，包括使2-氧代酸酯衍生物 在式（II）化合物的存在下，式R 1 -C（O）-C（O）-O-R 2（II））与选自脱氢酶类的酶（E） 被酶还原成式（I）的化合物，并且在反应过程中消耗的还原等价物通过在酶的帮助下将还原剂（RA）转化成相应的氧化产物（OP）再次再生（ E）。 本发明还包括在相应的S- 2-羟基酸酯的还原当量存在下还原2-氧代酸酯的脱氢酶，以及编码脱氢酶的核酸。

公开(公告)号：ES2377907T3

公开(公告)日：2012-04-03

申请号：ES08774090

申请日：2008-06-16

Applicant: BASF SE ,  MAX PLANCK GESELLSCHAFT ,  UNIV ALBERT LUDWIGS FREIBURG ,  MAX PLANCK GESELLSCHAFT

Inventor： BREUER MICHAEL ,  RABUS RALF ,  HEIDER JOHANN

IPC: C12P7/02

Abstract: Procedimiento para la obtención de alcoholes con actividad óptica de la fórmula Ia o Ib Fórmula Ia Fórmula Ib donde R1, R2 representan grupos alquilo, alquenilo, arilo o alquilarilo, que pueden estar substituidos a su vez una o varias veces por alquilo, halógeno, SH, SR2, OH, OR2, NO2, CN, CO, COOR2, NR2R3 o NR2R3R+X, representando R2, R3 y R4, independientemente entre sí, H o un resto alquilo inferior o alcoxi inferior con 1 a 6 átomos de carbono, y representando X' un contraión, con la medida de que R1 sea diferente a R2, mediante reducción de la correspondiente cetona, llevándose a cabo la reducción con una deshidrogenasa con la secuencia de polipéptidos SEQ ID NO: 2, o con una secuencia de polipéptidos, en la que hasta un 25 % de restos aminoácido frente a SEQ ID NO: 2 se han modificado mediante deleción, inserción, sustitución, o una combinación de los mismos.

公开(公告)号：CA2691799C

公开(公告)日：2016-06-07

申请号：CA2691799

申请日：2008-06-16

Applicant: BASF SE ,  ALBERT-LUDWIGS-UNIVERSITAET FREIBURG ,  MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WSS E V

Inventor： BREUER MICHAEL ,  RABUS RALF ,  HEIDER JOHANN

IPC: C12P7/02

Abstract: A method for producing optically active alcohols of formula (Ia) or (Ib), wherein R1, R2 are alkyl, alkenyl, aryl, or alkylaryl groups, which in turn can be mono-substituted or polsubstituted with alkyl, halogen, SH, SR2, OH OR2, NO2, CN, CO, COOR2, NR2R3 or NR2R3R4+ X, wherein R2 , R3 and R4 independently of each other are H or a low-alkyl or low-alkoxy radical, and X is a counterion, with the stipulation that R1 is not identical to R2, by reduction of the corresponding ketone, the reduction being performed with a dehydrogenase having the polypeptide sequence SEQ ID NO:2 or NO:4, or having a polypeptide sequence in which up to 25% of the amino acid radicals are modified relative to SEQ ID NO:2 or NO:4 by deletion, insertion, substitution, or a combination thereof.

公开(公告)号：AT535608T

公开(公告)日：2011-12-15

申请号：AT08774090

申请日：2008-06-16

Applicant: BASF SE ,  MAX PLANCK GESELLSCHAFT

Inventor： BREUER MICHAEL ,  RABUS RALF ,  HEIDER JOHANN

IPC: C12P7/02

Abstract: A process for preparing optically active alcohols of the formula Ia or Ib in which R1,R2 are alkyl, alkenyl, aryl, or alkylaryl groups which may in turn be substituted one or more times by alkyl, halogen, SH, SR2, OH, OR2, NO2, CN, CO, COOR2, NR2R3 or NR2R3R4+X, where R2, R3 and R4 are independently of one another H or a lower alkyl or lower alkoxy radical, and X−is a counter ion, with the proviso that R1 is not equal to R2, by reducing the corresponding ketone, where the reduction is carried out with a dehydrogenase having the polypeptide sequence of SEQ ID NO: 2 or NO: 4, or with a polypeptide sequence in which up to 25% of the amino acid residues are altered by comparison with SEQ ID NO: 2 or NO: 4 by deletion, insertion, substitution or a combination thereof.

公开(公告)号：MX2009014055A

公开(公告)日：2010-04-27

申请号：MX2009014055

申请日：2008-06-16

Applicant: BASF SE

Inventor： BREUER MICHAEL ,  RABUS RALF ,  HEIDER JOHANN

IPC: C12P7/02

Abstract: Un proceso para preparar alcoholes ?pticamente activos de la f?rmula la o Ib (ver f?rmulas) F?rmula la F?rmula Ib en la que R1,R2 son grupos alquilo, alquenilo, arilo, o alquilarilo, que a su vez pueden estar substituidos una o m?s veces por alquilo, hal?geno, SH, SR2, OH, OR2, NO2, CN, CO, COOR2, NR2R3 o NR2R3R4+X en donde R2, R3 y R4 son independientemente uno del otro H o un radical alquilo inferior o alcoxi inferior, y X es un contra ion, con la condici?n de que R1 no sea igual a R2, reduciendo la cetona correspondiente, en donde la reducci?n se lleva a cabo con una dehidrogenasa que tiene la secuencia de polip?ptido de SEC ID NO: 2 o NO: 4, o con una secuencia de polip?ptido en la que hasta 25% de los residuos de amino?cido se alteran mediante comparaci?n con SEC ID NO: 2 o NO: 4 mediante omisi?n, inserci?n, substituci?n o una combinaci?n de los mismos.

公开(公告)号：CA2691799A1

公开(公告)日：2008-12-24

申请号：CA2691799

申请日：2008-06-16

Applicant: BASF SE

Inventor： BREUER MICHAEL ,  HEIDER JOHANN ,  RABUS RALF

IPC: C12P7/02

Abstract: A method for producing optically active alcohols of formula (Ia) or (Ib), wherein R1, R2 are alkyl, alkenyl, aryl, or alkylaryl groups, which in turn can be mono-substituted or poly-substituted with alkyl, halogen, SH, SR2, O H, OR2, NO2, CN, CO, COOR2, NR2R3 or NR2R3R4+X, wherein R2, R3 and R4 indepe ndently of each other are H or a low-alkyl or low-alkoxy radical, and X- is a counterion, with the stipulation that R1 is not identical to R2, by reduct ion of the corresponding ketone, the reduction being performed with a dehydr ogenase having the polypeptide sequence SEQ ID NO:2 or NO:4, or having a pol ypeptide sequence in which up to 25% of the amino acid radicals are modified relative to SEQ ID NO:2 or NO:4 by deletion, insertion, substitution, or a combination thereof.