公开(公告)号：JP2012228257A

公开(公告)日：2012-11-22

申请号：JP2012151968

申请日：2012-07-06

Applicant: Basf Se ,  ビーエーエスエフ ソシエタス・ヨーロピアBasf Se

Inventor： BREUER MICHAEL ,  HAUER BERNHARD ,  SCHWANEBERG ULRICH ,  WONG TUCK SENG

IPC: C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N9/16 ,  C12P41/00

CPC classification number: C12N9/16 ,  C12P7/62 ,  C12P41/003

Abstract: PROBLEM TO BE SOLVED: To provide novel proteins exhibiting esterase activity and mutants thereof, nucleic acid sequences coding therefor, expression cassettes, vectors and recombinant microorganisms.SOLUTION: There are disclosed a method for producing the proteins obtained by starting from Pseudomonas glumae (Burkholderia plantarii) LU 2023 and the use of the proteins for the enzymatic, in particular, enantioselective ester hydrolysis or interesterification of organic esters.

Abstract translation: 要解决的问题：提供表现出酯酶活性的新蛋白质及其突变体，编码它们的核酸序列，表达盒，载体和重组微生物。 解决方案：公开了一种生产通过从假单胞菌（Burkholderia plantarii）LU 2023开始获得的蛋白质的方法，以及使用蛋白质进行酶促，特别是有机酯的对映选择性酯水解或酯交换。 版权所有（C）2013，JPO＆INPIT

公开(公告)号：JP2011142908A

公开(公告)日：2011-07-28

申请号：JP2011005361

申请日：2011-01-14

Applicant: Basf Se ,  ビーエーエスエフ ソシエタス・ヨーロピアBasf Se

Inventor： STUERMER RAINER ,  KESSELER MARIA ,  HAUER BERNHARD ,  FRIEDRICH THOMAS ,  BREUER MICHAEL

IPC: C12N15/09 ,  C07B61/00 ,  C07D333/16 ,  C07D333/20 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/04 ,  C12N15/53 ,  C12N15/63 ,  C12P7/04 ,  C12P17/00

CPC classification number: C07D333/16

Abstract: PROBLEM TO BE SOLVED: To provide an enzymatic or nonenzymatic method for producing 3-methylamino-1-(thien-2-yl)propan-1-ol represented by formula (I), and further, to provide an enzyme for conducting the method, a nucleic acid sequence encoding the enzyme, an expression cassette including the nucleic acid sequence, a vector and a recombinant host organism.
SOLUTION: The method comprises the following processes: (a) thiophene is reacted with a halogenated β-halopropionyl or halogenated acryloyl in the presence of a Lewis acid; wherein, either simultaneously or after the reaction but prior to isolating a reaction product, a hydrogen halide is passed through the reaction product to obtain a 3-halo-1-(thien-2-yl)propan-1-one; and (b) the propanone obtained in the step (a) is reduced, and subsequently, the reduced product is reacted with methylamine without isolating the reaction product as appropriate.
COPYRIGHT: (C)2011,JPO&INPIT

Abstract translation: 待解决的问题：提供用于制备由式（I）表示的3-甲基氨基-1-（噻吩-2-基）丙-1-醇的酶或非酶法，并且还提供用于 进行该方法，编码该酶的核酸序列，包含该核酸序列的表达盒，载体和重组宿主生物。 解决方案：该方法包括以下方法：（a）在路易斯酸存在下，噻吩与卤代β-卤代丙酰基或卤代丙烯酰基反应; 其中，在反应同时或之后但在分离反应产物之前，使卤化氢通过反应产物，得到3-卤代-1-（噻吩-2-基）丙-1-酮; 和（b）步骤（a）中获得的丙酮被还原，随后将还原产物与甲胺反应，而不适当地分离反应产物。 版权所有（C）2011，JPO＆INPIT

公开(公告)号：WO2014030096A3

公开(公告)日：2014-04-17

申请号：PCT/IB2013056604

申请日：2013-08-13

Applicant: BASF SE ,  EVOLVA SA ,  BASF CHINA CO LTD

Inventor： NAESBY MICHAEL ,  FOLLY CHRISTOPHE ,  NIELSEN CURT AIMÉ FRIIS ,  HATSCH ANAELLE ,  SCHWAB MARKUS ,  ZELDER OSKAR ,  HAEFNER STEFAN ,  SCHRÖDER HARTWIG ,  HOFF BIRGIT ,  MOLT ANDREA ,  DITRICH KLAUS ,  BREUER MICHAEL ,  HARTMANN HOLGER ,  KÖRBER KARSTEN

IPC: C12N1/21 ,  A61K31/48 ,  C07K14/195 ,  C07K14/37 ,  C07K16/00 ,  C12N1/15 ,  C12N1/16 ,  C12N15/31 ,  C12N15/52 ,  C12N15/80 ,  C12N15/81 ,  C12P1/00 ,  C12P17/10

CPC classification number: C12P17/12 ,  C07D209/90 ,  C07D487/06 ,  C07K16/40 ,  C12N9/0004 ,  C12N9/001 ,  C12N9/1007 ,  C12N9/1085 ,  C12N9/90 ,  C12N15/52 ,  C12P17/10 ,  C12P17/182 ,  C12Y205/01

Abstract: Microorganisms and processes for the recombinant manufacture of clavine-type alkaloids such as cycloclavine, festuclavine, agroclavine, chanoclavine and chanoclavine aldehyde, as well as polypeptides, polynucleotides and vectors comprising such polynucleotides which can be applied in a method for the manufacture of clavine-type alkaloids are provided.

Abstract translation: 用于重组生产角鲨毒碱生物碱如环圈子，festuclavine，agroclavine，chanoclavine和chanoclavine醛的微生物和方法，以及多肽，多核苷酸和包含这样的多核苷酸的载体，其可以用于制造棒状的方法 提供生物碱。

公开(公告)号：WO2012066059A2

公开(公告)日：2012-05-24

申请号：PCT/EP2011070304

申请日：2011-11-17

Applicant: BASF SE ,  BREUER MICHAEL ,  HAUER BERNHARD ,  JENDROSSEK DIETER ,  SIEDENBURG GABRIELE ,  PLEISS JUERGEN ,  SIRIM DEMET ,  RACOLTA SILVIA

Inventor： BREUER MICHAEL ,  HAUER BERNHARD ,  JENDROSSEK DIETER ,  SIEDENBURG GABRIELE ,  PLEISS JUERGEN ,  SIRIM DEMET ,  RACOLTA SILVIA

IPC: C12N9/90 ,  C07C29/56 ,  C12N15/52

CPC classification number: C12N9/90 ,  C07C29/17 ,  C07C29/56 ,  C07C2601/14 ,  C12P7/02 ,  C07C35/17 ,  C07C35/12

Abstract: The invention relates to novel types of mutants with cyclase-activity and to the use thereof in a method for the biocatalytic cyclisation of terpenes, such as, in particular, for producing isopulegol by the cyclisation of citronellal; to a method for producing menthol and methods for the biocatalytic conversion of other compounds with terpene type structural motifs.

Abstract translation: 本发明涉及一种具有环化酶活性以及它们在用于萜烯，如特别是用于通过环化香茅醛生产异胡薄荷醇的生物催化环化方法中使用的突变体新颖; 用于制备薄荷醇以及程序其他化合物与类似萜烯的结构基序的生物催化转化的方法。

公开(公告)号：WO2010139719A3

公开(公告)日：2011-02-24

申请号：PCT/EP2010057696

申请日：2010-06-02

Applicant: BASF SE ,  BREUER MICHAEL ,  HOERSTER ANDREA ,  HAUER BERNHARD

Inventor： BREUER MICHAEL ,  HOERSTER ANDREA ,  HAUER BERNHARD

IPC: C12P17/04 ,  C12N9/90

CPC classification number: C12P17/04 ,  C12N9/90

Abstract: The invention relates to a method for the biocatalytic production of ambroxan by means of a polypeptide having the activity of a homofarnesol-ambroxan cyclase and representing a novel class of enzymes.

Abstract translation: 本发明通过一种多肽的手段具有Homofarnesol-Ambroxan的活性环化酶，其代表一类新的酶涉及用于生物催化生产Ambroxan的方法。

公开(公告)号：WO2014041011A3

公开(公告)日：2014-05-08

申请号：PCT/EP2013068786

申请日：2013-09-11

Applicant: BASF SE ,  BASF SCHWEIZ AG ,  UNIV GRONINGEN

Inventor： BALDENIUS KAI-UWE ,  DITRICH KLAUS ,  BREUER MICHAEL ,  NAVICKAS VAIDOTAS ,  DICK JANSEN ,  BARTSCH SEBASTIAN ,  CRISMARU CIPRIAN GICA

IPC: C12P13/00 ,  C12N9/04 ,  C12N9/06 ,  C12N9/10

CPC classification number: C12N9/1096 ,  C12N9/0006 ,  C12P13/001 ,  C12Y101/01001 ,  C12Y104/01001 ,  C12Y206/01018

Abstract: The present invention relates to a novel enzymatically catalyzed method for the production of aliphatic primary amines, which method comprises the enzymatic oxidation of a primary aliphatic alcohol catalyzed by an alcohol dehydrogenase, amination of the resulting oxocompound catalyzed by a transaminase and optionally coupled with an enzymatically catalyzed regeneration of the consumed cofactor; recombinant expression systems and microorganisms procuring the required enzyme activities; and bioreactors for performing such methods; as well as the use of the obtained amines in organic synthesis, in particular for preparing organic polymers.

Abstract translation: 本发明涉及用于生产脂族伯胺的新的酶催化方法，该方法包括由醇脱氢酶催化的伯脂肪醇的酶氧化，由转氨酶催化的所得氧化合物的胺化，并任选地与酶 消耗的辅因子的催化再生; 重组表达系统和微生物获得所需的酶活性; 和用于进行这种方法的生物反应器; 以及在有机合成中使用得到的胺，特别是制备有机聚合物。

公开(公告)号：WO2008055988A3

公开(公告)日：2008-07-31

申请号：PCT/EP2007062170

申请日：2007-11-09

Applicant: BASF SE ,  BREUER MICHAEL ,  ERNST HANSGEORG ,  HAUER BERNHARD

Inventor： BREUER MICHAEL ,  ERNST HANSGEORG ,  HAUER BERNHARD

IPC: C12P23/00 ,  C12N9/04

CPC classification number: C12P23/00 ,  C12P7/02

Abstract: The invention relates to a method for production of optically active (4S)-4-hydroxy-2,6,6-trimethyl-cyclohex-2-ene-1-one derivatives of formula (I) and a method for production of (3S, 3'S)-astaxanthine of formula (III) including the above method.

Abstract translation: 本发明涉及一种用于光学活性的（4S）的制备-4-羟基-2,6,6-三甲基 - 环己-2-烯-1-酮的式（I）和用于制备的方法衍生物（ 3S，3'S） - 虾青素，包括前者的方法的式（III）的。

公开(公告)号：WO2010031776A3

公开(公告)日：2010-09-10

申请号：PCT/EP2009061974

申请日：2009-09-15

Applicant: BASF SE ,  BREUER MICHAEL ,  PLETSCH ANDREAS ,  HAUER BERNHARD ,  SIEGEL WOLFGANG

Inventor： BREUER MICHAEL ,  PLETSCH ANDREAS ,  HAUER BERNHARD ,  SIEGEL WOLFGANG

IPC: C12P7/22 ,  C12P13/00

CPC classification number: C12P13/001 ,  C12P7/22

Abstract: The present invention relates to a multi-stage process for producing substituted, optically active alcohols, comprising an enzyme-catalyzed synthesis step, in particular a synthesis step which is catalyzed by an alcohol dehydrogenase. The inventive method is particularly suitable for producing phenylephrine, i.e. 3-[(1R)-1-hydroxy-2-methylamino-ethyl]-phenol.

Abstract translation: 本发明涉及一种多级方法，其包括酶催化，特别是醇脱氢酶的催化下合成步骤被取代的，光学活性醇的制备方法。 特别地，对于苯福林的制备本发明的方法是合适的，即 3 - [（1R）-1-羟基-2-甲基氨基 - 乙基] - 苯酚。

公开(公告)号：WO2014086702A3

公开(公告)日：2014-09-12

申请号：PCT/EP2013075212

申请日：2013-12-02

Applicant: BASF SE

Inventor： BREUER MICHAEL ,  BLANK BENOIT

IPC: C12P5/00 ,  C07D307/44 ,  C12N9/04 ,  C12P7/00 ,  C12P17/04

CPC classification number: C07D307/44 ,  C12N9/0006 ,  C12P17/04

Abstract: The present invention relates to a biocatalytic process for preparing furandimethanols from hydroxymethylfurfurals, catalysed by suitable alcohol dehydrogenase enzymes, and to the use of the dimethanols thus prepared for preparation of or in polyesters, bisphenol A substitutes, resins, binders, polyethers, solvents, amines.

Abstract translation: 本发明涉及一种生物催化方法生产从Hydroxymethylfurfuralen Furandimethanolen通过合适的醇脱氢酶的酶和使用Dimethanole的用于生产或在聚酯，双酚A替代材料，树脂，粘合剂，聚醚，溶剂，胺这样制备的催化。

公开(公告)号：EP2888351A4

公开(公告)日：2016-04-27

申请号：EP13831449

申请日：2013-08-13

Applicant: BASF SE ,  EVOLVA SA

Inventor： NAESBY MICHAEL ,  FOLLY CHRISTOPHE ,  NIELSEN CURT AIMÉ FRIIS ,  HATSCH ANAELLE ,  SCHWAB MARKUS ,  ZELDER OSKAR ,  HAEFNER STEFAN ,  SCHRÖDER HARTWIG ,  HOFF BIRGIT ,  MOLT ANDREA ,  DITRICH KLAUS ,  BREUER MICHAEL ,  HARTMANN HOLGER ,  KÖRBER KARSTEN

IPC: C12N15/52 ,  C12N9/00 ,  C12N9/10 ,  C12N9/88 ,  C12N9/90 ,  C12P17/18

CPC classification number: C12P17/12 ,  C07D209/90 ,  C07D487/06 ,  C07K16/40 ,  C12N9/0004 ,  C12N9/001 ,  C12N9/1007 ,  C12N9/1085 ,  C12N9/90 ,  C12N15/52 ,  C12P17/10 ,  C12P17/182 ,  C12Y205/01

Abstract: Microorganisms and processes for the recombinant manufacture of clavine-type alkaloids such as cycloclavine, festuclavine, agroclavine, chanoclavine and chanoclavine aldehyde, as well as polypeptides, polynucleotides and vectors comprising such polynucleotides which can be applied in a method for the manufacture of clavine-type alkaloids are provided.

Abstract translation: 用于重组生产角鲨毒碱生物碱如环圈子，festuclavine，agroclavine，chanoclavine和chanoclavine醛的微生物和方法，以及多肽，多核苷酸和包含这样的多核苷酸的载体，其可以用于制造棒状的方法 提供生物碱。