公开(公告)号：CA2887300A1

公开(公告)日：2016-10-02

申请号：CA2887300

申请日：2015-04-02

Applicant: BASF SE

Inventor： KNAPP ANDREAS ,  VOGET SONJA ,  DANIEL ROLF ,  JAEGER KARL-ERICH

IPC: C12N15/55 ,  C07K14/195 ,  C12N1/21 ,  C12N9/16 ,  C12N9/20 ,  C12N15/113 ,  C12N15/31 ,  C12N15/63 ,  C12N15/67

Abstract: The present invention relates to the improved production of proteins, preferably enzymes such as lipases. In particular, the invention relates to a mutated signal peptide and nucleotide sequence encoding said signal peptide that results in an increased protein secretion. Further, the invention relates to a mutated promoter that results in an increased protein expression. It was surprisingly found that the combination of the mutated signal peptide and the mutated promoter act synergistically to result in an about 100-fold increased protein production. Nucleic acid molecules, expression vectors and host cells comprising the mutated signal peptide, mutated promoter, or the combination thereof are also encompassed by the invention. Finally, the invention relates to methods and uses of such nucleic acid molecules, expression vectors and host cells for protein preparation.

公开(公告)号：DE502006002664D1

公开(公告)日：2009-03-05

申请号：DE502006002664

申请日：2006-06-13

Applicant: BASF SE

Inventor： BESELIN ANKE ,  ROSENAU FRANK ,  JAEGER KARL-ERICH ,  BREUER MICHAEL ,  HAUER BERNHARD

IPC: C12N9/52

Abstract: The invention relates to novel proteins, especially with protease activity, which promote the production of extracellular lipases by bacteria, especially of the genus Burkholderia, nucleic acid sequences encoding them, expression constructs containing them, hosts transformed with them, methods for the production of the proteins with protease activity and methods for the production of lipases, especially by bacteria of the genus Burkholderia.

公开(公告)号：AT420950T

公开(公告)日：2009-01-15

申请号：AT06754335

申请日：2006-06-13

Applicant: BASF SE

Inventor： BESELIN ANKE ,  ROSENAU FRANK ,  JAEGER KARL-ERICH ,  BREUER MICHAEL ,  HAUER BERNHARD

IPC: C12N9/52

Abstract: The invention relates to novel proteins, especially with protease activity, which promote the production of extracellular lipases by bacteria, especially of the genus Burkholderia, nucleic acid sequences encoding them, expression constructs containing them, hosts transformed with them, methods for the production of the proteins with protease activity and methods for the production of lipases, especially by bacteria of the genus Burkholderia.

公开(公告)号：DK1893755T3

公开(公告)日：2009-04-20

申请号：DK06754335

申请日：2006-06-13

Applicant: BASF SE

Inventor： BESELIN ANKE ,  ROSENAU FRANK ,  HAUER BERNHARD ,  JAEGER KARL-ERICH ,  BREUER MICHAEL

IPC: C12N9/52

Abstract: The invention relates to novel proteins, especially with protease activity, which promote the production of extracellular lipases by bacteria, especially of the genus Burkholderia, nucleic acid sequences encoding them, expression constructs containing them, hosts transformed with them, methods for the production of the proteins with protease activity and methods for the production of lipases, especially by bacteria of the genus Burkholderia.