Abstract:
The present invention relates to a modified microorganism having, compared to its wild-type, a reduced activity of the enzyme that is encoded by the wcaJ-gene. The present invention also relates to a method for producing an organic compound and to the use of a modified microorganism.
Abstract:
The present invention relates to a modified microorganism having, compared to its wildtype, -a reduced activity of an enzyme encoded by the ptsA-gene, -a reduced activity of an enzyme encoded by the ptsH-gene or -a reduced activity of an enzyme encoded by the ptsA-gene and a reduced activity of an enzyme encoded by the ptsH-gene, wherein the wildtype from which the modified microorganism has been derived belongs to the family of Pasteurellaceae. The present invention also relates to a method for producing succinic acid and to the use of modified microorganisms.
Abstract:
A modified microorganism having, compared to its wildtype, an increased activity of the enzyme that is encoded by the alaD-gene is provided. A method for producing an alanine and the use of modified microorganisms are also provided.
Abstract:
Un microorganismo modificado de la familia Pasteurellaceae en el cual el gen wcaJ se ha delecionado o en el cual se ha introducido al menos una mutación en el gen wcaJ que conduce a la expresión de una enzima truncada codificada por el gen wcaJ en la cual al menos 100 aminoácidos de la enzima de tipo silvestre codificada por el gen wcaJ se delecionan del extremo C terminal, en el que el gen wcaJ comprende un ácido nucleico seleccionado del grupo que consiste en: a) ácidos nucleicos que tienen la secuencia nucleotídica de SEQ ID NO: 3; b) ácidos nucleicos que codifican la secuencia de aminoácidos de SEQ ID NO: 4; c) ácidos nucleicos que son al menos 70 % idénticos al ácido nucleico de a) o b), siendo la identidad la identidad en la longitud total de los ácidos nucleicos de a) o b); d) ácidos nucleicos que codifican una secuencia de aminoácidos que es al menos 70 % idéntica a la secuencia de aminoácidos codificada por el ácido nucleico de a) o b), siendo la identidad la identidad en la longitud total de la secuencia de aminoácidos codificada por los ácidos nucleicos de a) o b), e) ácidos nucleicos capaces de hibridar en condiciones rigurosas con una secuencia complementaria de cualquiera de los ácidos nucleicos de acuerdo con a) o b); y f) ácidos nucleicos que codifican la misma proteína que cualquiera de los ácidos nucleicos de a) o b), pero que difieren de los ácidos nucleicos de a) o b) anteriores debido a la degeneración del código genético.
Abstract:
The present invention relates to a modified microorganism having, compared to its wild-type, a reduced activity of the enzyme that is encoded by the wcaJ-gene. The present invention also relates to a method for producing an organic compound and to the use of a modified microorganism.
Abstract:
The present invention relates to a modified microorganism having, compared to its wild-type, a reduced activity of the enzyme that is encoded by the pykA-gene. The present invention also relates to a method for producing an organic compound and to the use of modified microorganisms.
Abstract:
Un microorganismo modificado que tiene, en comparación con su tipo silvestre, una actividad reducida de la enzima que está codificada por el gen pykA, en el que la actividad de la enzima codificada por el gen pykA se reduce por modificación genética, en el que la reducción de la actividad la enzima codificada por el gen pykA está en el intervalo de 15 a 99 %, en el que el tipo silvestre del cual se deriva el microorganismo modificado pertenece a la familia de Pasteurellaceae y en el que el tipo silvestre se refiere al microorganismo de origen natural que no ha sido modificado genéticamente.
Abstract:
The present invention relates to a process for producing an organic acid by fermentation, comprising the process steps: I)cultivating microorganisms in a culture medium to which are fed, as assimilable carbon sources, glycerol and a further carbonaceous compound, to allow the microorganisms to produce the organic acid, thereby obtaining a fermentation broth comprising the organic acid; II) recovering the organic acid or the salt thereof from the fermentation broth obtained in process step I); wherein, at least for a certain period of time in process step I), the consumption rate of the further carbonaceous compound (CRc.c.; in g per liter per hour) is lower than the maximum theoretical consumption rate of the further carbonaceous compound (CRc.c. max; in g per liter per hour).
Abstract:
The present invention relates to a modified microorganismhaving, compared to its wild-type, a reduced activity of the enzyme that is encoded bythe fruA-gene. The present invention also relates to a method for producing an organic compount and to the use of modified microorganisms.