公开(公告)号：WO2012050642A3

公开(公告)日：2012-04-19

申请号：PCT/US2011/041032

申请日：2011-06-20

Applicant: BATTELLE MEMORIAL INSTITUTE ,  SUN, Xuefei ,  KELLY, Ryan T. ,  TANG, Keqi ,  SMITH, Richard D.

Inventor： SUN, Xuefei ,  KELLY, Ryan T. ,  TANG, Keqi ,  SMITH, Richard D.

IPC: G01N27/26 ,  G01N27/447 ,  G01N35/10

Abstract: Microchip capillary electrophoresis (CE) utilizing a sample injector based on a mechanical valve rather than electrokinetic injection can provide improved sample injections, enhanced capabilities, and can eliminate the need for changing the electric field in the separation channel to induce sample injection. In one instance CE electrodes continuously apply an electric field for CE separation along a separation channel. A sample channel is connected to the separation channel at an intersection and has a sample pressure that is greater than that which is present in the separation channel near the intersection. The sample channel does not have electrodes that apply voltages for electrokinetic injection. A sample injector in the sample channel or at the intersection comprises a mechanical valve to control sample injection from the sample channel to the separation channel.

公开(公告)号：WO2014109812A1

公开(公告)日：2014-07-17

申请号：PCT/US2013/066773

申请日：2013-10-25

Applicant: BATTELLE MEMORIAL INSTITUTE

Inventor： SHI, Tujin ,  QIAN, Weijun ,  FILLMORE, Thomas L. ,  SUN, Xuefei ,  SMITH, Richard D.

IPC: C40B30/04

CPC classification number: G01N33/6848 ,  G01N30/72 ,  G01N30/8631 ,  G01N33/50 ,  G01N33/6842 ,  G01N2030/8831 ,  G01N2458/15 ,  G01N2560/00

Abstract: Antibody-free processes are disclosed that provide accurate quantification of a wide variety of low-abundance target analytes in complex samples. The processes can employ high-pressure, high resolution chromatographic separations for analyte enrichment. Intelligent selection of target fractions may be performed via on line Selected Reaction Monitoring (SRM) or off-line rapid screening of internal standards. Quantification may be performed on individual or multiplexed fractions. Applications include analyses of, e.g., very low abundance proteins or candidate biomarkers in plasma, cell, or tissue samples without the need for affinity specific reagents.

Abstract translation: 公开了无抗体方法，其提供复杂样品中各种低丰度目标分析物的准确定量。 该过程可以采用高压，高分辨率色谱分离来进行分析物富集。 目标分数的智能选择可以通过在线选择反应监测（SRM）或离线快速筛选内部标准进行。 可以对单个或多个分数进行定量。 应用包括例如血浆，细胞或组织样品中非常低丰度蛋白或候选生物标志物的分析，而不需要亲和力特异性试剂。