公开(公告)号：WO1994019693A1

公开(公告)日：1994-09-01

申请号：PCT/US1994001539

申请日：1994-02-09

Applicant: BAXTER DIAGNOSTICS INC.

Inventor： BAXTER DIAGNOSTICS INC. ,  LIN, Spencer, H. ,  YU, Kwok, Sum ,  SINGH, Pratap ,  DIAMOND, Steven, E.

IPC: G01N33/545

CPC classification number: G01N33/54306 ,  G01N33/545 ,  G01N33/552 ,  Y10S436/817 ,  Y10S436/823 ,  Y10S530/811

Abstract: Methods and compositions are provided for specific binding assays in which specific binding reagents are immobilized on a solid phase. Immobilization is facilitated by covalently coupling specific binding assay reagents such as polypeptide receptors or analytes with water soluble polymers. Such water soluble polymers, for example star polymers such as dendrimers, provide production advantages of lot-to-lot uniformity and homogeneity, and can enhance sensitivity due to low non-specific binding to the solid phase.

Abstract translation: 提供了用于特异性结合试验的方法和组合物，其中特异性结合试剂固定在固相上。 通过将特异性结合测定试剂如多肽受体或分析物与水溶性聚合物共价偶联来促进固定化。 这样的水溶性聚合物，例如星形聚合物，例如树枝状聚合物，提供了批次均匀性和均匀性的生产优点，并且由于低固定相的非特异性结合而提高了灵敏度。

公开(公告)号：WO1995008771A1

公开(公告)日：1995-03-30

申请号：PCT/US1994010032

申请日：1994-09-07

Applicant: BAXTER DIAGNOSTICS INC.

Inventor： BAXTER DIAGNOSTICS INC. ,  LIN, Spencer, H. ,  SINGH, Pratap ,  DIAMOND, Steven, E.

IPC: G01N33/543

CPC classification number: C07K16/3007 ,  C07K16/26 ,  C07K16/40 ,  G01N33/54353

Abstract: Methods and compositions are provided for specific binding assays in which specific binding reagents are immobilized on a solid phase. Immobilization is facilitated by coupling bifunctional reagents to specific binding assay proteins thereby chemically cross-linking the specific binding assay reagents. Such cross-linked specific binding assay reagents provide the advantage of performing assays without the use of secondary reagents. This provides for enhanced sensitivity through minimizing nonspecific background interference with the solid phase or with the target samples.

Abstract translation: 提供了用于特异性结合试验的方法和组合物，其中特异性结合试剂固定在固相上。 通过将双功能试剂偶联到特异性结合测定蛋白，从而化学交联特异性结合测定试剂来促进固定化。 这种交联特异性结合测定试剂提供了在不使用第二试剂的情况下进行测定的优点。 这通过最小化与固相或目标样品的非特异性背景干扰来提供增强的灵敏度。

公开(公告)号：WO1994019691A1

公开(公告)日：1994-09-01

申请号：PCT/US1994001906

申请日：1994-02-14

Applicant: BAXTER DIAGNOSTICS INC.

Inventor： BAXTER DIAGNOSTICS INC. ,  SINGH, Pratap ,  JOHNSON, Randy, W.

IPC: G01N33/53

CPC classification number: C12Q1/42 ,  G01N33/54393 ,  G01N33/581

Abstract: A composition and method are disclosed to reduce the effects that endogenous alkaline phosphatase found in some human bodily fluid samples have on solid phase immunoassay systems. The composition comprises a detergent, inhibitors to alkaline phosphatase, and a buffer and may contain a substrate to alkaline phosphatase.

Abstract translation: 公开了一种组合物和方法，以减少在一些人体液样品中发现的内源性碱性磷酸酶对固相免疫测定系统的影响。 组合物包含洗涤剂，碱性磷酸酶抑制剂和缓冲液，并且可以含有碱性磷酸酶的底物。

公开(公告)号：EP0637385A1

公开(公告)日：1995-02-08

申请号：EP94910108.0

申请日：1994-02-09

Applicant: BAXTER DIAGNOSTICS INC.

Inventor： LIN, Spencer, H. ,  YU, Kwok, Sum ,  SINGH, Pratap ,  DIAMOND, Steven, E.

IPC: G01N33

CPC classification number: G01N33/54306 ,  G01N33/545 ,  G01N33/552 ,  Y10S436/817 ,  Y10S436/823 ,  Y10S530/811

Abstract: Methods and compositions are provided for specific binding assays in which specific binding reagents are immobilized on a solid phase. Immobilization is facilitated by covalently coupling specific binding assay reagents such as polypeptide receptors or analytes with water soluble polymers. Such water soluble polymers, for example star polymers such as dendrimers, provide production advantages of lot-to-lot uniformity and homogeneity, and can enhance sensitivity due to low non-specific binding to the solid phase.

公开(公告)号：EP0670040A1

公开(公告)日：1995-09-06

申请号：EP94928017.0

申请日：1994-09-07

Applicant: BAXTER DIAGNOSTICS INC.

Inventor： LIN, Spencer, H. ,  SINGH, Pratap ,  DIAMOND, Steven, E.

IPC: G01N33 ,  C07K16 ,  C12N11

CPC classification number: C07K16/3007 ,  C07K16/26 ,  C07K16/40 ,  G01N33/54353

Abstract: Methods and compositions are provided for specific binding assays in which specific binding reagents are immobilized on a solid phase. Immobilization is facilitated by coupling bifunctional reagents to specific binding assay proteins thereby chemically cross-linking the specific binding assay reagents. Such cross-linked specific binding assay reagents provide the advantage of performing assays without the use of secondary reagents. This provides for enhanced sensitivity through minimizing nonspecific background interference with the solid phase or with the target samples.

公开(公告)号：EP0636247A1

公开(公告)日：1995-02-01

申请号：EP94909759.0

申请日：1994-02-14

Applicant: BAXTER DIAGNOSTICS INC.

Inventor： SINGH, Pratap ,  JOHNSON, Randy, W.

IPC: G01N33 ,  C12Q1

CPC classification number: C12Q1/42 ,  G01N33/54393 ,  G01N33/581

Abstract: A composition and method are disclosed to reduce the effects that endogenous alkaline phosphatase found in some human bodily fluid samples have on solid phase immunoassay systems. The composition comprises a detergent, inhibitors to alkaline phosphatase, and a buffer and may contain a substrate to alkaline phosphatase.