公开(公告)号：DE69503903T2

公开(公告)日：1999-03-25

申请号：DE69503903

申请日：1995-06-13

Applicant: BAXTER INT

Inventor： TSENG-LAW JANET ,  KOBORI JOAN ,  AL-ABDALY FAHAD ,  GUILLERMO ROY ,  HELGERSON SAM ,  DEANS ROBERT

IPC: G01N33/53 ,  C07K1/04 ,  C07K5/113 ,  C07K7/06 ,  C07K7/08 ,  C07K7/64 ,  C07K14/705 ,  C07K16/28 ,  C07K16/30 ,  C12N5/00 ,  C12N5/06 ,  C12N15/00 ,  C12N15/09 ,  C12P21/08 ,  C12R1/91 ,  G01N33/569 ,  G01N33/577 ,  C07K14/52

Abstract: The invention provides a nonenzymic method for the release of cells which have been selected from a heterogeneous cell suspension by antibody-mediated binding to beads or other solid support. The method entails forming within the cell suspension a complex comprising the solid support linked to a primary monoclonal antibody, which in turn is bound to a cell surface antigen on the target cells. The complex is separated from the cell suspension, and then contacted with a specific peptide which binds to the primary antibody, displacing the antibody from the cell surface antigen, thereby releasing the target cell from the complex. The invention also provides methods for positive/negative cell selection wherein target cells having a first antigen are selected from a heterogeneous cell suspension containing undesired cells having a second antigen.

公开(公告)号：DE69503903D1

公开(公告)日：1998-09-10

申请号：DE69503903

申请日：1995-06-13

Applicant: BAXTER INT

Inventor： TSENG-LAW JANET ,  KOBORI JOAN ,  AL-ABDALY FAHAD ,  GUILLERMO ROY ,  HELGERSON SAM ,  DEANS ROBERT

IPC: G01N33/53 ,  C07K1/04 ,  C07K5/113 ,  C07K7/06 ,  C07K7/08 ,  C07K7/64 ,  C07K14/705 ,  C07K16/28 ,  C07K16/30 ,  C12N5/00 ,  C12N5/06 ,  C12N15/00 ,  C12N15/09 ,  C12P21/08 ,  C12R1/91 ,  G01N33/569 ,  G01N33/577 ,  C07K14/52

Abstract: The invention provides a nonenzymic method for the release of cells which have been selected from a heterogeneous cell suspension by antibody-mediated binding to beads or other solid support. The method entails forming within the cell suspension a complex comprising the solid support linked to a primary monoclonal antibody, which in turn is bound to a cell surface antigen on the target cells. The complex is separated from the cell suspension, and then contacted with a specific peptide which binds to the primary antibody, displacing the antibody from the cell surface antigen, thereby releasing the target cell from the complex. The invention also provides methods for positive/negative cell selection wherein target cells having a first antigen are selected from a heterogeneous cell suspension containing undesired cells having a second antigen.