公开(公告)号：WO9507466A3

公开(公告)日：1995-04-06

申请号：PCT/US9410124

申请日：1994-09-08

Applicant: BAXTER INT

Inventor： AL-ABDALY FAHAD A ,  CHANG YU-AN ,  GUILLERMO ROY ,  SCOUTEN WILLIAM H ,  SMITH ALAN K

IPC: G01N33/53 ,  C07K16/28 ,  C07K16/30 ,  C12N11/00 ,  C12N15/02 ,  C12P21/08 ,  G01N33/543 ,  G01N33/569 ,  G01N33/58 ,  G01N33/50 ,  G01N33/532

CPC classification number: G01N33/56972 ,  G01N33/58

Abstract: The invention provides a stable conjugate of a target cell binding protein, preferably an antibody, covalently linked to a biotin analog moiety for use in positive cell selection and release. Methods for positive cell selection and release using the stable conjugate are described.

公开(公告)号：DE69503903T2

公开(公告)日：1999-03-25

申请号：DE69503903

申请日：1995-06-13

Applicant: BAXTER INT

Inventor： TSENG-LAW JANET ,  KOBORI JOAN ,  AL-ABDALY FAHAD ,  GUILLERMO ROY ,  HELGERSON SAM ,  DEANS ROBERT

IPC: G01N33/53 ,  C07K1/04 ,  C07K5/113 ,  C07K7/06 ,  C07K7/08 ,  C07K7/64 ,  C07K14/705 ,  C07K16/28 ,  C07K16/30 ,  C12N5/00 ,  C12N5/06 ,  C12N15/00 ,  C12N15/09 ,  C12P21/08 ,  C12R1/91 ,  G01N33/569 ,  G01N33/577 ,  C07K14/52

Abstract: The invention provides a nonenzymic method for the release of cells which have been selected from a heterogeneous cell suspension by antibody-mediated binding to beads or other solid support. The method entails forming within the cell suspension a complex comprising the solid support linked to a primary monoclonal antibody, which in turn is bound to a cell surface antigen on the target cells. The complex is separated from the cell suspension, and then contacted with a specific peptide which binds to the primary antibody, displacing the antibody from the cell surface antigen, thereby releasing the target cell from the complex. The invention also provides methods for positive/negative cell selection wherein target cells having a first antigen are selected from a heterogeneous cell suspension containing undesired cells having a second antigen.

公开(公告)号：ES2121400T3

公开(公告)日：1998-11-16

申请号：ES95923835

申请日：1995-06-13

Applicant: BAXTER INT

Inventor： TSENG-LAW JANET ,  KOBORI JOAN A ,  AL-ABDALY FAHAD A ,  GUILLERMO ROY ,  HELGERSON SAM L ,  DEANS ROBERT J

IPC: G01N33/53 ,  C07K1/04 ,  C07K5/113 ,  C07K7/06 ,  C07K7/08 ,  C07K7/64 ,  C07K14/705 ,  C07K16/28 ,  C07K16/30 ,  C12N5/00 ,  C12N5/06 ,  C12N15/00 ,  C12N15/09 ,  C12P21/08 ,  C12R1/91 ,  G01N33/569 ,  G01N33/577 ,  C07K14/52

Abstract: The invention provides a nonenzymic method for the release of cells which have been selected from a heterogeneous cell suspension by antibody-mediated binding to beads or other solid support. The method entails forming within the cell suspension a complex comprising the solid support linked to a primary monoclonal antibody, which in turn is bound to a cell surface antigen on the target cells. The complex is separated from the cell suspension, and then contacted with a specific peptide which binds to the primary antibody, displacing the antibody from the cell surface antigen, thereby releasing the target cell from the complex. The invention also provides methods for positive/negative cell selection wherein target cells having a first antigen are selected from a heterogeneous cell suspension containing undesired cells having a second antigen.

公开(公告)号：CA2192455C

公开(公告)日：2007-11-20

申请号：CA2192455

申请日：1995-06-13

Applicant: BAXTER INT

Inventor： TSENG-LAW JANET ,  KOBORI JOAN A ,  AL-ABDALY FAHAD A ,  GUILLERMO ROY ,  HELGERSON SAM L ,  DEANS ROBERT J

IPC: C07K16/00 ,  G01N33/53 ,  C07K1/04 ,  C07K5/113 ,  C07K7/06 ,  C07K7/08 ,  C07K7/64 ,  C07K14/00 ,  C07K14/705 ,  C07K16/28 ,  C07K16/30 ,  C12N1/02 ,  C12N5/00 ,  C12N5/06 ,  C12N5/08 ,  C12N5/12 ,  C12N15/00 ,  C12N15/09 ,  C12P21/08 ,  C12R1/91 ,  G01N33/543 ,  G01N33/554 ,  G01N33/566 ,  G01N33/569 ,  G01N33/577

Abstract: The invention provides a non-enzymatic method for the release of cells which have beem positively selected from a heterogeneous cell suspension by antibody-mediated binding to beads or other solid support . The method entails forming within the cell suspension a complex comprising the solid support linked to a primary monoclonal antibody , which in tum is bound to a cell surface antigen on the target cells. The complex is separated from the cell suspension, and then contacted with a specific peptide which binds to the primary antibody, displacing the antibody from the cell surface antigen, thereby releasing the target cell from the complex. The invention also provides methods for positive/negative cell selection wherein target cells having a first antigen are selected from a heterogeneous cell suspension containing undesired cells having a second antigen. The invention also provides methods for identifying a specific peptide useful for the release of a target cell from the binding of a specific monoclonal antibody. The methods of the invention are particularly useful for the positive selection of CD34+ hematopoietic stem cells and the concomitant purging of undesired tumor cells or lymphocytes from the positively selected cell population. The purified CD34+ cell composition is then usefulfor reinfusion to a cancer patient after high-dose therapy in order to reconstitute the patient's immune system.

公开(公告)号：DE69503903D1

公开(公告)日：1998-09-10

申请号：DE69503903

申请日：1995-06-13

Applicant: BAXTER INT

Inventor： TSENG-LAW JANET ,  KOBORI JOAN ,  AL-ABDALY FAHAD ,  GUILLERMO ROY ,  HELGERSON SAM ,  DEANS ROBERT

IPC: G01N33/53 ,  C07K1/04 ,  C07K5/113 ,  C07K7/06 ,  C07K7/08 ,  C07K7/64 ,  C07K14/705 ,  C07K16/28 ,  C07K16/30 ,  C12N5/00 ,  C12N5/06 ,  C12N15/00 ,  C12N15/09 ,  C12P21/08 ,  C12R1/91 ,  G01N33/569 ,  G01N33/577 ,  C07K14/52

Abstract: The invention provides a nonenzymic method for the release of cells which have been selected from a heterogeneous cell suspension by antibody-mediated binding to beads or other solid support. The method entails forming within the cell suspension a complex comprising the solid support linked to a primary monoclonal antibody, which in turn is bound to a cell surface antigen on the target cells. The complex is separated from the cell suspension, and then contacted with a specific peptide which binds to the primary antibody, displacing the antibody from the cell surface antigen, thereby releasing the target cell from the complex. The invention also provides methods for positive/negative cell selection wherein target cells having a first antigen are selected from a heterogeneous cell suspension containing undesired cells having a second antigen.

公开(公告)号：AU697627B2

公开(公告)日：1998-10-15

申请号：AU2826195

申请日：1995-06-13

Applicant: BAXTER INT

Inventor： TSENG-LAW JANET ,  KOBORI JOAN A ,  AL-ABDALY FAHAD A ,  GUILLERMO ROY ,  HELGERSON SAM L ,  DEANS ROBERT J

IPC: G01N33/53 ,  C07K1/04 ,  C07K5/113 ,  C07K7/06 ,  C07K7/08 ,  C07K7/64 ,  C07K14/705 ,  C07K16/28 ,  C07K16/30 ,  C12N5/00 ,  C12N5/06 ,  C12N15/00 ,  C12N15/09 ,  C12P21/08 ,  C12R1/91 ,  G01N33/569 ,  G01N33/577 ,  C07K14/52

Abstract: The invention provides a nonenzymic method for the release of cells which have been selected from a heterogeneous cell suspension by antibody-mediated binding to beads or other solid support. The method entails forming within the cell suspension a complex comprising the solid support linked to a primary monoclonal antibody, which in turn is bound to a cell surface antigen on the target cells. The complex is separated from the cell suspension, and then contacted with a specific peptide which binds to the primary antibody, displacing the antibody from the cell surface antigen, thereby releasing the target cell from the complex. The invention also provides methods for positive/negative cell selection wherein target cells having a first antigen are selected from a heterogeneous cell suspension containing undesired cells having a second antigen.

公开(公告)号：CA2192455A1

公开(公告)日：1995-12-21

申请号：CA2192455

申请日：1995-06-13

Applicant: BAXTER INT

Inventor： TSENG-LAW JANET ,  KOBORI JOAN A ,  AL-ABDALY FAHAD A ,  GUILLERMO ROY ,  HELGERSON SAM L ,  DEANS ROBERT J

IPC: G01N33/53 ,  C07K1/04 ,  C07K5/113 ,  C07K7/06 ,  C07K7/08 ,  C07K7/64 ,  C07K14/705 ,  C07K16/28 ,  C07K16/30 ,  C12N5/00 ,  C12N5/06 ,  C12N15/00 ,  C12N15/09 ,  C12P21/08 ,  C12R1/91 ,  G01N33/569 ,  G01N33/577 ,  C07K16/00 ,  C12N5/08 ,  C12N5/12 ,  G01N33/543 ,  G01N33/554 ,  G01N33/566

Abstract: The invention provides a non-enzymatic method for the release of cells which have beem positively selected from a heterogeneous cell suspension by antibodymediated binding to beads or other solid support. The method entails forming within the cell suspension a complex comprising the solid support linked to a primary monoclonal antibody, which in turn is bound to a cell surface antigen on the target cells. The complex is separated from the cell suspension, and then contacted with a specific peptide which binds to the primary antibody, displacing the antibody from the cell surface antigen, thereby releasing the target cell from the complex. The invention also provides methods for positive/negative cell selection wherein target cells having a first antigen are selected from a heterogeneous cell suspension containing undesired cells having a second antigen. The invention also provides methods for identifying a specific peptide useful for the release of a target cell from the binding of a specific monoclonal antibody. The methods of the invention are particularly useful for the positive selection of CD34+ hematopoietic stem cells and the concomitant purging of undesired tumor cells or lymphocytes from the positively selected cell population. The purified CD34+ cell composition is then useful for reinfusion to a cancer patient after high-dose therapy in order to reconstitute the patient's immune system.

公开(公告)号：CA2168205A1

公开(公告)日：1995-03-16

申请号：CA2168205

申请日：1994-09-08

Applicant: BAXTER INT

Inventor： AL-ABDALY FAHAD A ,  CHANG YU-AN ,  GUILLERMO ROY ,  SCOUTEN WILLIAM H ,  SMITH ALAN K

IPC: G01N33/53 ,  C07K16/28 ,  C07K16/30 ,  C12N11/00 ,  C12N15/02 ,  C12P21/08 ,  G01N33/543 ,  G01N33/569 ,  G01N33/58 ,  C12N5/00 ,  C07K1/13 ,  C12N5/08

Abstract: The invention provides a stable conjugate of a target cell binding protein, preferably an antibody, covalently linked to a biotin analog moiety for use in positive cell selection and release. The invention also provides a method for forming a stable conjugate of an antibody and a biotin analog moiety comprising bringing an N-hydroxysuccinimide (NHS) ester of a biotin analog into reactive contact with an antibody in a medium with a pH of about 8 and at a temperature of about 2.degree.-8.degree.C. The invention also provides methods for the positive selection of target cells from a heterogeneous cell suspension. One method involves the formation within the cell suspension of a complex formed by the target cell bound to a primary antibody linked to a biotin analog moiety, a secondary anti-biotin antibody bound to the biotin analog moiety and linked to a cell separation means. The complex is separated from the heterogeneous cell suspension, then incubated with a competitive compound, preferably authentic biotin, for which the secondary antibody has a higher affinity than it has for the biotin analog. The competitive compound displaces the biotin analog moiety from the binding site of the secondary antibody, thus releasing the target cells from the complex. The cell separation means may then be separated from the target cells. Another method involves the formation within the heterogeneous cell suspension of a complex formed by the target cell bound to a primary antibody linked to a biotin analog moiety and a form of avidin, which is bound to the biotin analog moiety. The complex is separated from the heterogeneous cell suspension and then incubated with a competitive compound, preferably authentic biotin, for which avidin has a higher affinity than it has for the biotin analog. The competitive compound displaces the biotin analog moiety from the avidin binding site, thus releasing the target cells from the complex. The avidin may then be separated from the target cells. The invention also provides compositions of matter which are intermediate complexes in the above methods. One intermediate complex comprises a cell separation means bound to an anti-biotin antibody, which in turn is bound to a biotin analog moiety covalently linked to a target cell binding protein. Another intermediate complex comprises avidin bound to a biotin analog moiety covalently linked to a target cell binding protein.

公开(公告)号：AT169409T

公开(公告)日：1998-08-15

申请号：AT95923835

申请日：1995-06-13

Applicant: BAXTER INT

Inventor： TSENG-LAW JANET ,  KOBORI JOAN A ,  AL-ABDALY FAHAD A ,  GUILLERMO ROY ,  HELGERSON SAM L ,  DEANS ROBERT J

IPC: G01N33/53 ,  C07K1/04 ,  C07K5/113 ,  C07K7/06 ,  C07K7/08 ,  C07K7/64 ,  C07K14/705 ,  C07K16/28 ,  C07K16/30 ,  C12N5/00 ,  C12N5/06 ,  C12N15/00 ,  C12N15/09 ,  C12P21/08 ,  C12R1/91 ,  G01N33/569 ,  G01N33/577 ,  C07K14/52

Abstract: The invention provides a nonenzymic method for the release of cells which have been selected from a heterogeneous cell suspension by antibody-mediated binding to beads or other solid support. The method entails forming within the cell suspension a complex comprising the solid support linked to a primary monoclonal antibody, which in turn is bound to a cell surface antigen on the target cells. The complex is separated from the cell suspension, and then contacted with a specific peptide which binds to the primary antibody, displacing the antibody from the cell surface antigen, thereby releasing the target cell from the complex. The invention also provides methods for positive/negative cell selection wherein target cells having a first antigen are selected from a heterogeneous cell suspension containing undesired cells having a second antigen.

公开(公告)号：AU7832094A

公开(公告)日：1995-03-27

申请号：AU7832094

申请日：1994-09-08

Applicant: BAXTER INT

Inventor： AL-ABDALY FAHAD A ,  CHANG YU-AN ,  GUILLERMO ROY ,  SCOUTEN WILLIAM H ,  SMITH ALAN K

IPC: G01N33/53 ,  C07K16/28 ,  C07K16/30 ,  C12N11/00 ,  C12N15/02 ,  C12P21/08 ,  G01N33/543 ,  G01N33/569 ,  G01N33/58 ,  G01N33/50 ,  G01N33/532