公开(公告)号：WO2014144868A1

公开(公告)日：2014-09-18

申请号：PCT/US2014/029460

申请日：2014-03-14

Applicant: BECKMAN COULTER, INC.

Inventor： GRAHAM, Marshall Donnie ,  GRAHAM, William Gerry ,  CLARKIN, James P. ,  WELLS, Mark A. ,  CANO, Jose M. ,  ARBOLEDA, Carlos Alberto ,  SANCHEZ, Armando J.

IPC: G01N15/14 ,  C03B23/047

CPC classification number: G01N21/05 ,  C03B23/047 ,  C03C27/00 ,  G01N15/1434 ,  G01N2015/1006 ,  G01N2015/1452 ,  G01N2015/1486 ,  G01N2015/149 ,  G01N2201/02 ,  Y02P40/57

Abstract: An improved optical flow cell adapted for use in a flow cytometer for differentiating formed bodies (e.g., blood cells) in liquid suspensions. Preferably manufactured by assembling, aligning, and optically joining at least two elements made from transparent material, the improved flow cell has a seamless internal flow channel of preferably non-circular cross-section in a cylindrical first element through which prepared samples can be metered and an independent second element having an external envelope suited to acquisition of optical parameters from formed bodies in such suspensions, the second element being conforming and alignable to the first element so that non-axisymmetric refractive effects on optical characterizing parameters of formed bodies passing through the flow channel in the first element may be minimized before the two elements are optically joined and fixed in working spatial relationship.

Abstract translation: 一种改进的光学流通池，适用于流式细胞仪，用于分离液体悬浮液中的成形体（例如血细胞）。 优选通过组装，对准和光学地连接由透明材料制成的至少两个元件来制造，改进的流动池具有在圆柱形第一元件中优选非圆形横截面的无缝内部流动通道，通过该圆形第一元件可以计量准备的样品， 独立的第二元件具有适合于在这种悬架中从成形体获取光学参数的外部包络线，所述第二元件与第一元件一致并对齐，使得对通过流动的成形体的光学特性参数的非轴对称折射效应 在两个元件以工作空间关系被光学地连接和固定之前，第一元件中的通道可以被最小化。

公开(公告)号：EP2972209B1

公开(公告)日：2019-08-28

申请号：EP14721665.9

申请日：2014-03-14

Applicant: Beckman Coulter, Inc.

Inventor： GRAHAM, Marshall Donnie ,  GRAHAM, William Gerry ,  CLARKIN, James P. ,  WELLS, Mark A. ,  CANO, Jose M. ,  ARBOLEDA, Carlos Alberto ,  SANCHEZ, Armando J.

IPC: G01N15/14 ,  C03B23/047 ,  C03C27/00 ,  G01N21/05 ,  G01N15/10

公开(公告)号：EP2972209A1

公开(公告)日：2016-01-20

申请号：EP14721665.9

申请日：2014-03-14

Applicant: Beckman Coulter, Inc.

Inventor： GRAHAM, Marshall Donnie ,  GRAHAM, William Gerry ,  CLARKIN, James P. ,  WELLS, Mark A. ,  CANO, Jose M. ,  ARBOLEDA, Carlos Alberto ,  SANCHEZ, Armando J.

IPC: G01N15/14 ,  C03B23/047

CPC classification number: G01N21/05 ,  C03B23/047 ,  C03C27/00 ,  G01N15/1434 ,  G01N2015/1006 ,  G01N2015/1452 ,  G01N2015/1486 ,  G01N2015/149 ,  G01N2201/02 ,  Y02P40/57

Abstract: An improved optical flow cell adapted for use in a flow cytometer for differentiating formed bodies (e.g., blood cells) in liquid suspensions. Preferably manufactured by assembling, aligning, and optically joining at least two elements made from transparent material, the improved flow cell has a seamless internal flow channel of preferably non-circular cross-section in a cylindrical first element through which prepared samples can be metered and an independent second element having an external envelope suited to acquisition of optical parameters from formed bodies in such suspensions, the second element being conforming and alignable to the first element so that non-axisymmetric refractive effects on optical characterizing parameters of formed bodies passing through the flow channel in the first element may be minimized before the two elements are optically joined and fixed in working spatial relationship.

Abstract translation: 改进的光学流通池适用于流式细胞仪，用于分离液体悬浮液中的成形体（例如血细胞）。 优选地，通过组装，对准和光学地连接由透明材料制成的至少两个元件制造，改进的流动池具有在圆柱形第一元件中优选非圆形横截面的无缝内部流动通道，通过该圆形第一元件可以计量准备的样品， 独立的第二元件具有适合于在这种悬架中从成形体获取光学参数的外部包络线，第二元件与第一元件一致并对准，使得对通过流动的成形体的光学特性参数的非轴对称折射效应 在两个元件以工作空间关系被光学连接和固定之前，第一元件中的通道可以被最小化。