公开(公告)号：DE69219589T2

公开(公告)日：1997-09-11

申请号：DE69219589

申请日：1992-11-16

Applicant: BECKMAN INSTRUMENTS INC

Inventor： HSIEH YOU-ZUNG ,  CHEN FU-TAI ,  STERNBERG JAMES ,  KLIEN GERALD ,  LIU CHENG-MING

IPC: C12Q1/04 ,  C12Q1/00 ,  G01N27/447

Abstract: Disclosed herein is a methodology for analyzing isoenzymes using capillary zone electrophoresis ("CZE") techniques. Briefly, an isoenzyme-containing sample and a substrate capable of being catalyzed by said isoenzyme into a reaction product are introduced into a capillary column comprising a buffer. Most preferably, the buffer contains the substrate prior to introduction of the sample into such substrate-buffer. CZE separation techniques are applied to the column such that the isoenzymes are separated from each other into discrete zones. The separation techniques are terminated such that product is rapidly generated by the catalytic conversion of substrate by the isoenzymes, and accumulated, within each discrete zone, followed by detection of product. Information regarding the relative distribution of the isoenzymes can be derived from the relative distribution of the product.

公开(公告)号：DE69219589D1

公开(公告)日：1997-06-12

申请号：DE69219589

申请日：1992-11-16

Applicant: BECKMAN INSTRUMENTS INC

Inventor： HSIEH YOU-ZUNG ,  CHEN FU-TAI ,  STERNBERG JAMES ,  KLIEN GERALD ,  LIU CHENG-MING

IPC: C12Q1/04 ,  C12Q1/00 ,  G01N27/447

Abstract: Disclosed herein is a methodology for analyzing isoenzymes using capillary zone electrophoresis ("CZE") techniques. Briefly, an isoenzyme-containing sample and a substrate capable of being catalyzed by said isoenzyme into a reaction product are introduced into a capillary column comprising a buffer. Most preferably, the buffer contains the substrate prior to introduction of the sample into such substrate-buffer. CZE separation techniques are applied to the column such that the isoenzymes are separated from each other into discrete zones. The separation techniques are terminated such that product is rapidly generated by the catalytic conversion of substrate by the isoenzymes, and accumulated, within each discrete zone, followed by detection of product. Information regarding the relative distribution of the isoenzymes can be derived from the relative distribution of the product.