公开(公告)号：AU3876993A

公开(公告)日：1994-01-20

申请号：AU3876993

申请日：1993-05-24

Applicant: BECKMAN INSTRUMENTS INC

Inventor： LIU CHENG-MING ,  WANG HANN-PING ,  CHEN FU-TAI ALBERT ,  STERNBERG JAMES C ,  KLEIN GERALD L

IPC: B26D5/22 ,  B26F1/02 ,  B65H20/24 ,  G01N33/577 ,  G01N33/558 ,  G01N33/561 ,  G01N33/564

Abstract: A method for perforating a sheet material provides for a perforating die located intermediate two orbital rollers. The two orbital rollers move in orbital paths in the same orbital direction at the same speed and in positions which are 180 degrees apart. Each roller acts as a temporary storage input and output in opposite phase from each other so that incoming sheet material is alternately stored and dispensed as a loop on the orbital roller. Since the rollers are out of phase, this permits a segment of the sheet material to be stopped intermediate the orbital rollers so that the perforating die can act on the stopped segment.

公开(公告)号：DE69219589T2

公开(公告)日：1997-09-11

申请号：DE69219589

申请日：1992-11-16

Applicant: BECKMAN INSTRUMENTS INC

Inventor： HSIEH YOU-ZUNG ,  CHEN FU-TAI ,  STERNBERG JAMES ,  KLIEN GERALD ,  LIU CHENG-MING

IPC: C12Q1/04 ,  C12Q1/00 ,  G01N27/447

Abstract: Disclosed herein is a methodology for analyzing isoenzymes using capillary zone electrophoresis ("CZE") techniques. Briefly, an isoenzyme-containing sample and a substrate capable of being catalyzed by said isoenzyme into a reaction product are introduced into a capillary column comprising a buffer. Most preferably, the buffer contains the substrate prior to introduction of the sample into such substrate-buffer. CZE separation techniques are applied to the column such that the isoenzymes are separated from each other into discrete zones. The separation techniques are terminated such that product is rapidly generated by the catalytic conversion of substrate by the isoenzymes, and accumulated, within each discrete zone, followed by detection of product. Information regarding the relative distribution of the isoenzymes can be derived from the relative distribution of the product.

公开(公告)号：CA2143206A1

公开(公告)日：1995-01-19

申请号：CA2143206

申请日：1994-05-18

Applicant: BECKMAN INSTRUMENTS INC

Inventor： LIU CHENG-MING ,  WANG HANN-PING

IPC: B01J20/285 ,  C07K1/16 ,  C07K1/26 ,  G01N1/10 ,  G01N1/36 ,  G01N27/447 ,  G01N30/14 ,  G01N30/88 ,  G01N33/49 ,  G01N33/493 ,  G01N1/34 ,  G01N33/483 ,  G01N33/68

Abstract: Processes are provided for pretreating body fluid compositions and subsequently analyzing the pretreated body fluid compositions for analytes of interest. Processes for penetrating the compositions include providing size exclusion gel having a molecular weight fractionation range or a molecular weight exclusion such that the size exclusion gel is capable of excluding or fractionating the analytes of interest, and then causing the composition to contact the size exclusion gel in order to separate the analytes from low molecular weight composition components which interfere with the separation and analysis of the analytes of interest. Processes for analyzing pretreated compositions include electrophoretic methods such as capillary zone electrophoresis which involve the separation and detection of analytes of interest.

公开(公告)号：AU686495B2

公开(公告)日：1998-02-05

申请号：AU4646896

申请日：1995-12-20

Applicant: BECKMAN INSTRUMENTS INC

Inventor： KEO NIDA ,  SAFARIAN ZARA ,  LIU CHENG-MING ,  WANG HANN-PING

IPC: G01N33/72 ,  G01N27/447

Abstract: A buffer and method useful for the analysis of glycoproteins by capillary zone electrophoresis. The buffer comprises water, a sugar complexing compound, a base compound for adjusting the pH, and a zwitterionic compound. An embodiment of the buffer comprises sodium borate as the complexing compound, sodium hydroxide as the base, and 3-cyclohexylamino-1-propanesulfonic acid as the zwitterionic compound. In the method, a selected glycoprotein is subjected to capillary zone electrophoresis. The proportion or the amount of the glycoprotein is determined by quantitative analysis of the resulting electropherogram.

公开(公告)号：DE69219589D1

公开(公告)日：1997-06-12

申请号：DE69219589

申请日：1992-11-16

Applicant: BECKMAN INSTRUMENTS INC

Inventor： HSIEH YOU-ZUNG ,  CHEN FU-TAI ,  STERNBERG JAMES ,  KLIEN GERALD ,  LIU CHENG-MING

IPC: C12Q1/04 ,  C12Q1/00 ,  G01N27/447

Abstract: Disclosed herein is a methodology for analyzing isoenzymes using capillary zone electrophoresis ("CZE") techniques. Briefly, an isoenzyme-containing sample and a substrate capable of being catalyzed by said isoenzyme into a reaction product are introduced into a capillary column comprising a buffer. Most preferably, the buffer contains the substrate prior to introduction of the sample into such substrate-buffer. CZE separation techniques are applied to the column such that the isoenzymes are separated from each other into discrete zones. The separation techniques are terminated such that product is rapidly generated by the catalytic conversion of substrate by the isoenzymes, and accumulated, within each discrete zone, followed by detection of product. Information regarding the relative distribution of the isoenzymes can be derived from the relative distribution of the product.

公开(公告)号：AU669308B2

公开(公告)日：1996-05-30

申请号：AU7835394

申请日：1994-09-15

Applicant: BECKMAN INSTRUMENTS INC

Inventor： WANG HANN-PING ,  LIU CHENG-MING

IPC: G01N33/68 ,  G01N21/33 ,  G01N21/79 ,  G01N27/447 ,  G01N33/49

Abstract: A method of quantitating proteins in complex samples using capillary electrophoresis can be used both to determine the concentration of a protein in a sample and to determine total protein concentration in the sample. In general, the method of determining the concentration of a marker protein comprises: (1) adding a known quantity of an internal standard compound to a sample containing at least one protein, the internal standard compound selected from the group consisting of benzoic acid substituted with at least one halogen, producing a detector signal in relation to its concentration, and being capable of electrophoretic separation from the protein; (2) subjecting the sample and the internal standard compound to capillary electrophoresis to separate the protein and the internal standard compound from each other and from other components in the sample; (3) measuring the detector signal produced by the internal standard compound and a detector signal produced by the protein to determine a ratio of protein signal to internal standard signal; and (4) determining the concentration of the protein in the sample from a standard curve of protein concentration versus the ratio of protein signal to internal standard signal. A typical protein measurable by the method is albumin. Typically, measurements are made at 214 nm and the internal standard compound is 2,4-dichlorobenzoic acid.

公开(公告)号：CA2149903A1

公开(公告)日：1995-04-13

申请号：CA2149903

申请日：1994-09-15

Applicant: BECKMAN INSTRUMENTS INC

Inventor： WANG HANN-PING ,  LIU CHENG-MING

IPC: G01N33/68 ,  G01N21/33 ,  G01N21/79 ,  G01N27/447 ,  G01N27/26

Abstract: A method of quantitating proteins in complex samples using capillary electrophoresis can be used both to determine the concentration of a protein in a sample and to determine total protein concentration in the sample. In general, the method of determining the concentration of a marker protein comprises: (1) adding a known quantity of an internal standard compound to a sample containing at least one protein, the internal standard compound selected from the group consisting of benzoic acid substituted with at least one halogen, producing a detector signal in relation to its concentration, and being capable of electrophoretic separation from the protein; (2) subjecting the sample and the internal standard compound to capillary electrophoresis to separate the protein and the internal standard compound from each other and from other components in the sample; (3) measuring the detector signal produced by the internal standard compound and a detector signal produced by the protein to determine a ratio of protein signal to internal standard signal; and (4) determining the concentration of the protein in the sample from a standard curve of protein concentration versus the ratio of protein signal to internal standard signal. A typical protein measurable by the method is albumin. Typically, measurements are made at 214 nm and the internal standard compound is 2,4-dichlorobenzoic acid.

公开(公告)号：AT152775T

公开(公告)日：1997-05-15

申请号：AT92925261

申请日：1992-11-16

Applicant: BECKMAN INSTRUMENTS INC

Inventor： HSIEH YOU-ZUNG ,  CHEN FU-TAI A ,  STERNBERG JAMES C ,  KLIEN GERALD ,  LIU CHENG-MING

IPC: C12Q1/04 ,  C12Q1/00 ,  G01N27/447 ,  C12Q1/32 ,  C12Q1/50

Abstract: Disclosed herein is a methodology for analyzing isoenzymes using capillary zone electrophoresis ("CZE") techniques. Briefly, an isoenzyme-containing sample and a substrate capable of being catalyzed by said isoenzyme into a reaction product are introduced into a capillary column comprising a buffer. Most preferably, the buffer contains the substrate prior to introduction of the sample into such substrate-buffer. CZE separation techniques are applied to the column such that the isoenzymes are separated from each other into discrete zones. The separation techniques are terminated such that product is rapidly generated by the catalytic conversion of substrate by the isoenzymes, and accumulated, within each discrete zone, followed by detection of product. Information regarding the relative distribution of the isoenzymes can be derived from the relative distribution of the product.

公开(公告)号：AU4646896A

公开(公告)日：1996-08-07

申请号：AU4646896

申请日：1995-12-20

Applicant: BECKMAN INSTRUMENTS INC

Inventor： KEO NIDA ,  SAFARIAN ZARA ,  LIU CHENG-MING ,  WANG HANN-PING

IPC: G01N33/72 ,  G01N27/447

Abstract: A buffer and method useful for the analysis of glycoproteins by capillary zone electrophoresis. The buffer comprises water, a sugar complexing compound, a base compound for adjusting the pH, and a zwitterionic compound. An embodiment of the buffer comprises sodium borate as the complexing compound, sodium hydroxide as the base, and 3-cyclohexylamino-1-propanesulfonic acid as the zwitterionic compound. In the method, a selected glycoprotein is subjected to capillary zone electrophoresis. The proportion or the amount of the glycoprotein is determined by quantitative analysis of the resulting electropherogram.

公开(公告)号：CA2184267A1

公开(公告)日：1996-07-25

申请号：CA2184267

申请日：1995-12-20

Applicant: BECKMAN INSTRUMENTS INC

Inventor： KEO NIDA ,  SAFARIAN ZARA ,  LIU CHENG-MING ,  WANG HANN-PING

IPC: G01N33/72 ,  G01N27/447

Abstract: A buffer and method useful for the analysis of glycoproteins by capillary zone electrophoresis. The buffer comprises water, a sugar complexing compound, a base compound for adjusting the pH, and a zwitterionic compound. An embodiment of the buffer comprises sodium borate as the complexing compound, sodium hydroxide as the base, and 3-cyclohexylamino-1-propanesulfonic acid as the zwitterionic compound. In the method, a selected glycoprotein is subjected to capillary zone electrophoresis. The proportion or the amount of the glycoprotein is determined by quantitative analysis of the resulting electropherogram.