公开(公告)号：WO9401446A3

公开(公告)日：1994-03-03

申请号：PCT/US9306214

申请日：1993-06-29

Applicant: BECKMAN INSTRUMENTS INC

Inventor： REDDY PARAMESWARA M ,  MICHAEL MAGED A

IPC: C07H21/00

CPC classification number: C07H21/00

Abstract: A solid-phase nucleotide synthesis intermediate useful for chemical synthesis of oligodeoxyribonucleotides and oligoribonucleotides comprises: (1) a particulate support comprising a porous polymer whose backbone comprises optionally substituted acrylate or methacrylate moieties; (2) a nucleoside; and (3) a linker having a first and a second end, the first end being covalently attached to the particulate support and the second end being covalently attached to the nucleoside, the linker spacing the nucleoside at least 3 atoms away from the polymer. The linker can comprise at least one optionally substituted aliphatic diamine. Alternatively, the linker can comprise a polyethylene glycol moiety. Preferably, the porous polymer is a methacrylate-vinylidene polymer. The solid-phase support thus produced can be used for oligodeoxyribonucleotide synthesis by either the phosphite-triester or the phosphotriester processes.