公开(公告)号：DE3478509D1

公开(公告)日：1989-07-06

申请号：DE3478509

申请日：1984-11-16

Applicant: BECKMAN INSTRUMENTS INC

Inventor： CRAGLE LINDA ,  HARRIS PAUL ,  LEE SHIH-YUN ,  TUNG KER ,  VODIAN MORTON

IPC: A61K39/00 ,  C12Q1/00 ,  G01N33/53 ,  G01N33/543 ,  G01N33/577

Abstract: An immunoassay for assaying an antigenic substance (Ag) in a fluid. The immunoassay is the type which comprises contacting the fluid with at least one first entity selected from a group consisting of an antibody (Ab) to the Ag, a soluble, labeled antibody (L-Aba) to the Ag, and an antibody (Abb) to the Ag bound to a solid support (SC). The immunoassay is characterized in that the fluid is contacted with at least one additional entity selected from a group consisting of at least one different type of soluble, labeled antibody (L-Abc) to the Ag, at least one different type of antibody (Abd) bound to a solid carrier (SC1), and at least one different type of antibody (Abe) to the Ag. The SC1 is selected from a group consisting of SC, at least one different solid carrier (SC2), and mixtures thereof (SC and SC2). Each type of L-Abc, Abd-SC1, and Abe has a lower average affinity constant (K) for Ag than each respective K of L-Aba, Abb-SC and Ab e; and the additional entity is present in an amount sufficient to avoid a hook effect. Also, a reagent of the type comprising at least one first entity selected from a group consisting of L-Aba, Abb-SC, and Ab. The reagent is characterized in that it further comprises at least one additional entity selected from a group consisting of at least one different type of L-Abc and at least one different type of Abd-SC1, and at least one different type of Abe. Ab, L-Aba, Abb-SC, L-Abc, Abd-SC1, and Abe are as defined above.