公开(公告)号：DE3464013D1

公开(公告)日：1987-07-09

申请号：DE3464013

申请日：1984-07-23

Applicant: BECKMAN INSTRUMENTS INC

Inventor： COOK WILLIAM ,  VODIAN MORTON ,  HAUNOLD OTTO

IPC: B01L9/06 ,  B01L99/00 ,  G01N33/543 ,  G01N35/00 ,  B01L11/00

Abstract: A device for handling and washing beads. The device is attachable to a test tube rack and includes a frame, a containing slide, and a capture slide. By manipulating the slides in various combinations of positions the beads may be added to, removed from, or separately washed apart from test tubes in the rack. Complete and easy washing of the beads may thus be accomplished.

公开(公告)号：DE3478509D1

公开(公告)日：1989-07-06

申请号：DE3478509

申请日：1984-11-16

Applicant: BECKMAN INSTRUMENTS INC

Inventor： CRAGLE LINDA ,  HARRIS PAUL ,  LEE SHIH-YUN ,  TUNG KER ,  VODIAN MORTON

IPC: A61K39/00 ,  C12Q1/00 ,  G01N33/53 ,  G01N33/543 ,  G01N33/577

Abstract: An immunoassay for assaying an antigenic substance (Ag) in a fluid. The immunoassay is the type which comprises contacting the fluid with at least one first entity selected from a group consisting of an antibody (Ab) to the Ag, a soluble, labeled antibody (L-Aba) to the Ag, and an antibody (Abb) to the Ag bound to a solid support (SC). The immunoassay is characterized in that the fluid is contacted with at least one additional entity selected from a group consisting of at least one different type of soluble, labeled antibody (L-Abc) to the Ag, at least one different type of antibody (Abd) bound to a solid carrier (SC1), and at least one different type of antibody (Abe) to the Ag. The SC1 is selected from a group consisting of SC, at least one different solid carrier (SC2), and mixtures thereof (SC and SC2). Each type of L-Abc, Abd-SC1, and Abe has a lower average affinity constant (K) for Ag than each respective K of L-Aba, Abb-SC and Ab e; and the additional entity is present in an amount sufficient to avoid a hook effect. Also, a reagent of the type comprising at least one first entity selected from a group consisting of L-Aba, Abb-SC, and Ab. The reagent is characterized in that it further comprises at least one additional entity selected from a group consisting of at least one different type of L-Abc and at least one different type of Abd-SC1, and at least one different type of Abe. Ab, L-Aba, Abb-SC, L-Abc, Abd-SC1, and Abe are as defined above.