公开(公告)号：WO2005035785A3

公开(公告)日：2006-04-20

申请号：PCT/FR2004002558

申请日：2004-10-08

Applicant: BERTIN TECHNOLOGIES SA ,  CENTRE NAT RECH SCIENT ,  RAVASSARD PHILIPPE ,  BIZET KARINE ,  MALLET JACQUES ,  VERDIER AMANDINE

Inventor： RAVASSARD PHILIPPE ,  BIZET KARINE ,  MALLET JACQUES ,  VERDIER AMANDINE

IPC: C12Q1/68

CPC classification number: C12Q1/6816 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/689 ,  C12Q2563/125 ,  C12Q2527/125 ,  C12Q2565/519 ,  C12Q2527/113 ,  C12Q2525/204

Abstract: The invention relates to a method for the rapid analysis of a microbial population of a given environment, such as a microbial population sampled from the air. In particular, the inventive method comprises the use of DNA chips and a radioactive or chimiluminescent detection method.

Abstract translation: 本发明涉及用于快速分析给定环境的微生物群体的方法，例如从空气中抽取的微生物群体。 特别地，本发明的方法包括使用DNA芯片和放射性或致密发光检测方法。

公开(公告)号：WO0244740A3

公开(公告)日：2003-01-03

申请号：PCT/FR0103686

申请日：2001-11-21

Applicant: BERTIN TECHNOLOGIES SA ,  BIZET KARINE ,  VALLAYER BRUNO ,  VATTAIRE MAURICE

Inventor： BIZET KARINE ,  VALLAYER BRUNO ,  VATTAIRE MAURICE

IPC: G01N1/10 ,  G01N30/06 ,  G01N30/24 ,  G01N35/00 ,  G01N35/02 ,  G01N35/04 ,  G01N35/10

CPC classification number: G01N35/025 ,  G01N30/06 ,  G01N30/24 ,  G01N35/1016 ,  G01N2001/1006 ,  G01N2035/00366 ,  G01N2035/0441 ,  G01N2035/0443 ,  G01N2035/0444 ,  G01N2035/0446

Abstract: The invention concerns an immunoassay apparatus, comprising coaxial rotating trays (14) supporting reaction dishes (18) and (16) supporting tubes (24) containing samples to be analysed, means for driving said trays in rotation about a common axis, and means (28) supporting reagents, extending in an arc around said rotating trays.

Abstract translation: 本发明涉及一种免疫测定装置，包括支撑反应盘（18）的同轴旋转托盘（14）和（16）支撑待分析样品的管子（24），用于围绕公共轴线旋转驱动所述托盘的装置，以及装置 28）支持试剂，在围绕所述旋转盘的弧中延伸。

公开(公告)号：FR2866959A1

公开(公告)日：2005-09-02

申请号：FR0401965

申请日：2004-02-26

Applicant: BERTIN TECHNOLOGIES SA

Inventor： VALLAYER BRUNO ,  MAQUET PHILIPPE ,  BIZET KARINE

IPC: G01N30/88 ,  G01N35/00 ,  G01N35/02 ,  G01N35/04 ,  G01N35/10 ,  G01N33/53

Abstract: Automatic apparatus for immunological dosing comprises a rotating plate (18) designed to receive reaction well supports, and has a drive to rotate the plate and a distributor for putting samples and reagents into the wells. Automatic apparatus for immunological dosing comprises a rotating plate (18) designed to receive reaction well supports, and has a drive to rotate the plate and a distributor for putting samples and reagents into the wells. It is made to perform a particular sequence of actions in distributing the samples and reagents, heating and incubation, washing and reading results. The various systems for performing the sequence are mounted on a chassis (10) at predetermined angular positions around the plate's axis of rotation, the chassis being fitted with a series of positioning modules for the different systems. The plate also has fixing and positioning components (40, 42) for disposable sliders with sample tubes, reaction wells, and sample and reagent dispensers. Heat is provided by a warm air flow, and incubation is controlled by monitors.

公开(公告)号：CA2541911A1

公开(公告)日：2005-04-28

申请号：CA2541911

申请日：2004-10-13

Applicant: BERTIN TECHNOLOGIES SA

Inventor： MARON PIERRE-ALAIN ,  CARVALHO ESMERALDA ,  BIZET KARINE ,  LEJON DAVID

IPC: C12N15/10

公开(公告)号：FR2860802A1

公开(公告)日：2005-04-15

申请号：FR0408344

申请日：2004-07-28

Applicant: BERTIN TECHNOLOGIES SA ,  CENTRE NAT RECH SCIENT

Inventor： RAVASSARD PHILIPPE ,  BIZET KARINE ,  MALLET JACQUES ,  VERDIER AMANDINE

IPC: B01J19/00 ,  B01L3/00 ,  C12Q1/68 ,  G01N21/76 ,  G01N33/53

Abstract: Chemiluminescent reading of a DNA chip in which a porous membrane, impregnated with a chemiluminescent substrate (I) and serving to immobilize the liquid substrate, is placed on the chip surface, is new. An independent claim is also included for a chip for the new process comprising a thin layer of glass on which is deposited capture probes (DNA or RNA specific for microorganisms) and a porous membrane impregnated with (I).

公开(公告)号：FR2860801A1

公开(公告)日：2005-04-15

申请号：FR0311921

申请日：2003-10-10

Applicant: BERTIN TECHNOLOGIES SA ,  CENTRE NAT RECH SCIENT

Inventor： RAVASSARD PHILIPPE ,  BIZET KARINE ,  MALLET JACQUES ,  VERDIER AMANDINE

IPC: C12Q1/68 ,  G01N33/58

Abstract: Rapid detection of microorganisms (A) comprising collecting (A); extracting RNA; labeling the RNA, or converting it to labeled DA; hybridizing the labeled nucleic acid to capture probes, specific for each microorganism, present on a chip, where hybridization takes only 15-45, preferably 30, minutes; detecting any hybridization and analysis of the results, is new.

公开(公告)号：AT419395T

公开(公告)日：2009-01-15

申请号：AT04817156

申请日：2004-10-08

Applicant: BERTIN TECHNOLOGIES SA ,  CENTRE NAT RECH SCIENT

Inventor： RAVASSARD PHILIPPE ,  BIZET KARINE ,  MALLET JACQUES ,  VERDIER AMANDINE

IPC: C12Q1/68

Abstract: Rapid detection of microorganisms (A) comprising collecting (A); extracting RNA; labeling the RNA, or converting it to labeled DA; hybridizing the labeled nucleic acid to capture probes, specific for each microorganism, present on a chip, where hybridization takes only 15-45, preferably 30, minutes; detecting any hybridization and analysis of the results, is new.

公开(公告)号：AT388229T

公开(公告)日：2008-03-15

申请号：AT04791526

申请日：2004-10-13

Applicant: BERTIN TECHNOLOGIES SA

Inventor： MARON PIERRE-ALAIN ,  LEJON DAVID ,  BIZET KARINE ,  CARVALHO ESMERALDA

IPC: C12N15/10

Abstract: Method for extracting nucleic acids from microorganisms using a combination of three lytic techniques. Method for extracting nucleic acids from microorganisms comprises: (a) lysing microorganisms (A) by, in any sequence, chemical lysis, using a detergent solution, thermal lysis by incubating the extract at below 0[deg]C and then immediately at over 95, preferably about 100, [deg]C, and mechanical lysis by stirring the extract in presence of beads; (b) precipitation of membrane debris and proteins; (c) recovering the nucleic acid (NA)-containing supernatant; (d) precipitation of NA and removal of supernatant; and (e) optionally redissolving NA in buffer. An independent claim is also included for a method for analyzing the microbial population of an environment by taking a sample; resuspension in solution; extracting NA by the new method, then analyzing NA.

公开(公告)号：FR2817350B1

公开(公告)日：2008-01-04

申请号：FR0015701

申请日：2000-11-29

Applicant: BERTIN TECHNOLOGIES SA

Inventor： BIZET KARINE ,  VALLAYER BRUNO ,  VATTAIRE MAURICE

IPC: G01N35/00 ,  G01N1/10 ,  G01N30/06 ,  G01N30/24 ,  G01N35/02 ,  G01N35/04 ,  G01N35/10

公开(公告)号：FR2861085A1

公开(公告)日：2005-04-22

申请号：FR0312049

申请日：2003-10-15

Applicant: BERTIN TECHNOLOGIES SA

Inventor： MARON PIERRE ALAIN ,  LEJON DAVID ,  BIZET KARINE ,  CARVALHO ESMERALDA

IPC: C12N15/10

Abstract: Method for extracting nucleic acids from microorganisms using a combination of three lytic techniques. Method for extracting nucleic acids from microorganisms comprises: (a) lysing microorganisms (A) by, in any sequence, chemical lysis, using a detergent solution, thermal lysis by incubating the extract at below 0[deg]C and then immediately at over 95, preferably about 100, [deg]C, and mechanical lysis by stirring the extract in presence of beads; (b) precipitation of membrane debris and proteins; (c) recovering the nucleic acid (NA)-containing supernatant; (d) precipitation of NA and removal of supernatant; and (e) optionally redissolving NA in buffer. An independent claim is also included for a method for analyzing the microbial population of an environment by taking a sample; resuspension in solution; extracting NA by the new method, then analyzing NA.