公开(公告)号：FR2861085A1

公开(公告)日：2005-04-22

申请号：FR0312049

申请日：2003-10-15

Applicant: BERTIN TECHNOLOGIES SA

Inventor： MARON PIERRE ALAIN ,  LEJON DAVID ,  BIZET KARINE ,  CARVALHO ESMERALDA

IPC: C12N15/10

Abstract: Method for extracting nucleic acids from microorganisms using a combination of three lytic techniques. Method for extracting nucleic acids from microorganisms comprises: (a) lysing microorganisms (A) by, in any sequence, chemical lysis, using a detergent solution, thermal lysis by incubating the extract at below 0[deg]C and then immediately at over 95, preferably about 100, [deg]C, and mechanical lysis by stirring the extract in presence of beads; (b) precipitation of membrane debris and proteins; (c) recovering the nucleic acid (NA)-containing supernatant; (d) precipitation of NA and removal of supernatant; and (e) optionally redissolving NA in buffer. An independent claim is also included for a method for analyzing the microbial population of an environment by taking a sample; resuspension in solution; extracting NA by the new method, then analyzing NA.

公开(公告)号：FR2861085B1

公开(公告)日：2006-01-27

申请号：FR0312049

申请日：2003-10-15

Applicant: BERTIN TECHNOLOGIES SA

Inventor： MARON PIERRE ALAIN ,  LEJON DAVID ,  BIZET KARINE ,  CARVALHO ESMERALDA

IPC: C12N15/10

Abstract: Method for extracting nucleic acids from microorganisms using a combination of three lytic techniques. Method for extracting nucleic acids from microorganisms comprises: (a) lysing microorganisms (A) by, in any sequence, chemical lysis, using a detergent solution, thermal lysis by incubating the extract at below 0[deg]C and then immediately at over 95, preferably about 100, [deg]C, and mechanical lysis by stirring the extract in presence of beads; (b) precipitation of membrane debris and proteins; (c) recovering the nucleic acid (NA)-containing supernatant; (d) precipitation of NA and removal of supernatant; and (e) optionally redissolving NA in buffer. An independent claim is also included for a method for analyzing the microbial population of an environment by taking a sample; resuspension in solution; extracting NA by the new method, then analyzing NA.