公开(公告)号：JPH08233824A

公开(公告)日：1996-09-13

申请号：JP32890195

申请日：1995-12-18

Applicant: BIO RAD LABORATORIES

Inventor： DONARUDO JIEI BAATORINGU ,  SERUGEI BURASENKO

IPC: G01N33/50 ,  B01J20/281 ,  G01N30/74 ,  G01N30/78 ,  G01N30/88 ,  G01N33/72 ,  G01N30/48

Abstract: PROBLEM TO BE SOLVED: To provide a method enabling continuous measurements for the content of saccharified hemoglobin in blood without regenerating an ion exchange column between a previous measuring sample and a coming measuring sample. SOLUTION: A boronate-affinitive absorption column 23 is utilized as an analyzer column. A series of hemolytic samples 11 is injected in a carrier liquid at 12, 13 every predetermined time interval. An absorbance is measured by a spectrophotometer 21 with the light of a wavelength absorbed by the hemoglobin before and after a liquid flow 14 including the samples pass through the column. Two measuring values are compared with each other, thereby to calculate the content of saccharified hemoglobin. Accordingly, 100 or more samples can be analyzed without detaching/regenerating the column.