公开(公告)号：JPH03239959A

公开(公告)日：1991-10-25

申请号：JP3597490

申请日：1990-02-16

Applicant: BIO RAD LABORATORIES

Inventor： MIN DE ZUU ,  JIENNCHII CHIEN ,  SUTERAN JIYAATEN

IPC: G01N27/447

Abstract: PURPOSE: To make it possible to separate a mixture of sample ions with good reproducibility and stability by causing it to pass a separating column electrophoretically containing an aqueous solution without a gel of a linear polymer having a specific molecular weight. CONSTITUTION: A mixture of sample ions of different molecular weights in a sample is caused to pass a separating column electrophoretically containing an aqueous solution without a gel of a practically linear polymer having a molecular weight of about 10 to about 2×10 . At this time, its molecular weight is in the range of about 0.1 to about 200 times as large as the average molecular weight of high polymers in the mixture, and is large enough to delay the flow of the high molecules passing the separating column to the extent that the concentration of the polymer in the solution changes together with the molecular weight of the high molecules. Besides, one having sample ions of macromolecules, and having a polymer concentration high enough to lengthen the high polymer holding time in at least about 25%/about 35%/about 50% columns is used. Besides, as a polymer one having an average molecular weight being between the minimum molecular weight and the maximum molecular weight of the sample ions in the mixture, is used.