公开(公告)号：JPH04264254A

公开(公告)日：1992-09-21

申请号：JP25532391

申请日：1991-10-02

Applicant: BIO RAD LABORATORIES

Inventor： ERITSUKU RAI ,  BURUUSU DABURIYU BAAREN ,  UIRU EICHI SUTABURUBAIN ,  JIYOOJI II BAASU

IPC: B01D57/02 ,  G01N27/447

Abstract: PURPOSE: To improve cataphoresis separation by receiving the support of a computer and utilizing a knowledge based on calibration data that are obtained by separating a gigantic molecule with a known size under conditions for optimizing separation. CONSTITUTION: A system 100 consisting of a computer system 150, a contour fixing homogeneous electric field(CHEF) mapper 120, and a cataphoresis device 130 operates in a complete automatic mode. A user inputs the minimum and maximum fragment sizes of a gigantic molecule to be separated, especially DNA. Related knowledge base is provided, thus determining optimized parameters of a forward voltage gradient, a backward voltage gradient, a voltage gradient, an angle, an initial switch time, a final switch time, and a run period. The mapper 120 can directly calibrate the device 130 using the parameters, thus separating the maximum gigantic molecule.