公开(公告)号：AU6716694A

公开(公告)日：1995-01-24

申请号：AU6716694

申请日：1994-05-04

Applicant: BIO RAD LABORATORIES

Inventor： BINDER STEVEN R ,  KING DAVID L

IPC: G01N30/02 ,  G01N30/06 ,  G01N30/08 ,  G01N30/14 ,  G01N30/34 ,  G01N30/46 ,  G01N30/74 ,  G01N30/86 ,  G01N30/88 ,  G01N33/94

Abstract: Biological samples are analyzed for benzodiazepines in a single isocratic analysis using a chromatographic column system containing an immobilized enzyme reactor which cleaves glucuronic acid-conjugated benzodiazepines, an anion exchange column, a hydrophobic cation exchange column and a reverse-phase analytical column. Preferred methods of performing the analysis further involve the use of a hydrophobic cation exchange precolumn prior to the anion exchange column. The system readily lends itself to automation, automatic periodic sampling and benzodiazepine identification and quantification. The system is particularly well adapted to the determination and identification of benzodiazepines in urine samples.

公开(公告)号：CA2153904A1

公开(公告)日：1995-01-12

申请号：CA2153904

申请日：1994-05-04

Applicant: BIO RAD LABORATORIES

Inventor： BINDER STEVEN R ,  KING DAVID L

IPC: G01N30/02 ,  G01N30/06 ,  G01N30/08 ,  G01N30/14 ,  G01N30/34 ,  G01N30/46 ,  G01N30/74 ,  G01N30/86 ,  G01N30/88 ,  G01N33/94 ,  C12S3/24 ,  G01N33/483

Abstract: Biological samples are analyzed for benzodiazepines in a single isocratic an alysis using a chromatographic column system containing an immobilized enzyme reactor which cleaves glucuronic acid-conjugated benzo diazepines, an anion exchange column, a hydrophobic cation exchange column and a reverse-phase analytical column. Preferred meth ods of performing the analysis further involve the use o f a hydrophobic cation exchange precolumn prior to the anion exchange column. The system readily lends itself to automation, automati c periodic sampling and benzodiazepine identification and quantification. The system is particularly well adapted to the determination and identification of benzodiazepines in urine samples.

公开(公告)号：AU668824B2

公开(公告)日：1996-05-16

申请号：AU6716694

申请日：1994-05-04

Applicant: BIO RAD LABORATORIES

Inventor： BINDER STEVEN R ,  KING DAVID L

IPC: G01N30/02 ,  G01N30/06 ,  G01N30/08 ,  G01N30/14 ,  G01N30/34 ,  G01N30/46 ,  G01N30/74 ,  G01N30/86 ,  G01N30/88 ,  G01N33/94 ,  G01N33/493

Abstract: Biological samples are analyzed for benzodiazepines in a single isocratic analysis using a chromatographic column system containing an immobilized enzyme reactor which cleaves glucuronic acid-conjugated benzodiazepines, an anion exchange column, a hydrophobic cation exchange column and a reverse-phase analytical column. Preferred methods of performing the analysis further involve the use of a hydrophobic cation exchange precolumn prior to the anion exchange column. The system readily lends itself to automation, automatic periodic sampling and benzodiazepine identification and quantification. The system is particularly well adapted to the determination and identification of benzodiazepines in urine samples.

公开(公告)号：AT163763T

公开(公告)日：1998-03-15

申请号：AT94914954

申请日：1994-05-04

Applicant: BIO RAD LABORATORIES

Inventor： BINDER STEVEN R ,  KING DAVID L

IPC: G01N30/02 ,  G01N30/06 ,  G01N30/08 ,  G01N30/14 ,  G01N30/34 ,  G01N30/46 ,  G01N30/74 ,  G01N30/86 ,  G01N30/88 ,  G01N33/94

Abstract: Biological samples are analyzed for benzodiazepines in a single isocratic analysis using a chromatographic column system containing an immobilized enzyme reactor which cleaves glucuronic acid-conjugated benzodiazepines, an anion exchange column, a hydrophobic cation exchange column and a reverse-phase analytical column. Preferred methods of performing the analysis further involve the use of a hydrophobic cation exchange precolumn prior to the anion exchange column. The system readily lends itself to automation, automatic periodic sampling and benzodiazepine identification and quantification. The system is particularly well adapted to the determination and identification of benzodiazepines in urine samples.