公开(公告)号：GB2193808A

公开(公告)日：1988-02-17

申请号：GB8716018

申请日：1987-07-08

Applicant: BIO RAD LABORATORIES

Inventor： LAMOTTE III GEORGE B

IPC: G01N33/569 ,  C12Q1/70

公开(公告)号：DE3722273A1

公开(公告)日：1988-01-21

申请号：DE3722273

申请日：1987-07-06

Applicant: BIO RAD LABORATORIES

Inventor： WALKER ROGER P ,  LAMOTTE III GEORGE B

IPC: G01N33/543 ,  G01N33/569

Abstract: Solid phase binding reagents for biochemical binding assays are prepared by isolating a plurality of selected binding species from a biological source mixture such as a cell or viral lysate; diluting each isolated species in a carrier liquid, such as a detergent-containing buffer solution, to form a series of stock mixtures each at a preselected concentration; and applying the mixtures to discrete, precisely defined regions on a solid phase support, immobilizing the species according to a preselected pattern. After suitable washing, blocking and stabilization steps, the resulting solid phase reagent may be used like a Western blot of an electropherogram, but with distinct advantages in terms of accuracy, reproducibility and readability.

公开(公告)号：DE3722272A1

公开(公告)日：1988-01-21

申请号：DE3722272

申请日：1987-07-06

Applicant: BIO RAD LABORATORIES

Inventor： LAMOTTE III GEORGE B

IPC: G01N33/569

Abstract: Antibodies to certain specific AIDS viral proteins, notably those having molecular weights of 41K-43K, and preferably also those having molecular weights of 120K and 24K are measured as a means of differentiating among individuals who have been exposed to AIDS to distinguish between those who have actually developed AIDS and those who have been exposed but have remained healthy. Statistically significant differences in the levels of antibodies binding to viral proteins at these three molecular weights have been proven to distinguish between these two exposure conditions, and may thus be used as a means of providing prognostic and/or diagnostic information regarding AIDS, rather than merely confirmatory information.

公开(公告)号：GB2193315A

公开(公告)日：1988-02-03

申请号：GB8716051

申请日：1987-07-08

Applicant: BIO RAD LABORATORIES

Inventor： FITZGERALD RONALD CHARLES ,  LAMOTTE III GEORGE B

IPC: B01L3/00 ,  G01N33/53 ,  G01N33/543 ,  G01N33/569 ,  C12Q1/70

公开(公告)号：GB2194046A

公开(公告)日：1988-02-24

申请号：GB8716020

申请日：1987-07-08

Applicant: BIO RAD LABORATORIES

Inventor： WALKER ROGER P ,  LAMOTTE III GEORGE B

IPC: G01N33/543 ,  G01N33/569 ,  G01N33/53 ,  G01N33/531 ,  G01N33/567

公开(公告)号：DE3722271A1

公开(公告)日：1988-01-21

申请号：DE3722271

申请日：1987-07-06

Applicant: BIO RAD LABORATORIES

Inventor： FITZGERALD RONALD CHARLES ,  LAMOTTE III GEORGE B

IPC: B01L3/00 ,  G01N33/53 ,  G01N33/543 ,  G01N33/569

Abstract: Antibodies having specificity for antigenic sites on AIDS-associated viruses are detected in a bodily fluid sample by incubating the sample with antigens from an AIDS virus immobilized on a solid support, associating with each of the antigen-antibody complexes a signal producing system, and determining the signal level from the resulting associated complexes. The signal producing system of particular interest is an anti-human IgG enzyme conjugate, notably alkaline phosphatase, in conjunction with Nitro, Blue Tetrazolium and 5 bromo-4 chloro 3 indolyl phosphate.