公开(公告)号：AU3024889A

公开(公告)日：1989-10-05

申请号：AU3024889

申请日：1989-02-23

Applicant: BIO RAD LABORATORIES

Inventor： DECHENE NEAL E ,  RATHBUN BARBARA J ,  REGAN BARBARA P ,  WALKER ROGER P

IPC: G01N33/547 ,  G01N33/552 ,  G01N33/569 ,  G01N33/571

Abstract: A biospecific assay method where detection of herpesviruses using silane-derivatized solid supports is disclosed. The solid supports are silanized to produce surface aminoalkyl, alkyl, or aminophenoxy groups, whereby adsorption of herpes virus particles onto the derivatized solid support is substantially increased. The method involves disrupting a herpesvirus in a sample with a solubilization agent, immobilizing the herpesvirus so disrupted on the derivatized solid support, contacting the immobilized herpesvirus with a primary antibody specific for the disrupted virus particle to form an immunocomplex, contacting the immunocomplex with a second antibody which has a label appended thereto to form a labeled immunocomplex, and detecting the labeled immunocomplex.