公开(公告)号：JPH02110373A

公开(公告)日：1990-04-23

申请号：JP16247389

申请日：1989-06-23

Applicant: BIO RAD LABORATORIES

Inventor： SHIYASHIDARA ETSUCHI EMU MAASH ,  MAIKERU AAUIN WATOKINSU

IPC: A61B10/00 ,  G01N33/53 ,  G01N33/543 ,  G01N33/547 ,  G01N33/569

Abstract: PURPOSE: To detect sperm antibody on the surface of a sperm by bringing a sperm cell into contact with latex beads that is bound to antihuman immunity globulin and examining the latex beads for the adhesion of beads to the sperm cell. CONSTITUTION: Latex beads is a synthetic water-insoluble polymer that can be dispersed in colloid shape into a water solution. A latex polymer is subjected to mono dispersion, is carboxylate modification polystyrene particle, and performs binding to liberated amine or carboxyl group that exists in immunity globulin. Crosslinking, spacer arm, or other binding parts are first coupled to the latex beads and can be used to adhere the beads to an antibody. They also increase a functional group. When antihuman immunity globulin that is coupled to the latex beads adheres human antibody to the latex beads in a buffer system, the antihuman immunity globulin reacts with human antibody, thus determining the presence of a sperm antibody that directly exists in a sperm.

公开(公告)号：JPH02247566A

公开(公告)日：1990-10-03

申请号：JP1047090

申请日：1990-01-19

Applicant: BIO RAD LABORATORIES

Inventor： SHIYASHIDARA ETSUCHI EMU MAASH ,  JIERARUDAIN TAN ZAN

IPC: C07D475/04 ,  C07D487/22 ,  G01N1/28 ,  G01N33/53 ,  G01N33/82

Abstract: PURPOSE: To enable easily, quickly and safely denaturating serum protein by processing serum sample before analyzing with microwave at the high dial. CONSTITUTION: A serum sample to be analyzed is arranged in a microwave oven and exposed to microwave. The microwave oven has dials of low, middle and high and the high dial means that the magnetron emits microwave at 80 to 100% of the time, the oven is operated. For the microwave for example, one generated at 700W is used. The sample is processed at the high dial for approximately 20 to 90 seconds (60 seconds is most proper) processed by microwave. The serum sample is thus processed with microwave for at least 15 to 20 seconds and exposed in the temperature approximately 65 to 100 deg.C (80 to 85 deg.C is most proper). The serum sample is processed with microwave at this temperature for 60 seconds or less. In this manner, denaturation is caused in existing B12 or folate without disturbance as a whole.