公开(公告)号：DE60030713D1

公开(公告)日：2006-10-26

申请号：DE60030713

申请日：2000-05-09

Applicant: BIO RAD LABORATORIES

Inventor： RAGSDALE W ,  TAGLIAMONTE JOHN

IPC: C12M1/00 ,  C12N15/64 ,  C12M1/42 ,  C12M3/00

公开(公告)号：CA2375362C

公开(公告)日：2006-10-31

申请号：CA2375362

申请日：2000-05-09

Applicant: BIO RAD LABORATORIES

Inventor： RAGSDALE CHARLES W ,  TAGLIAMONTE JOHN

IPC: C12M1/00 ,  C12N13/00 ,  A61N1/08 ,  A61N1/30 ,  C12M1/42 ,  C12N15/64 ,  G01N37/00

Abstract: An electroporation system and method for directing high-voltage currents to a suspension of cells contained in a cuvette. A high-voltage switch controls t he coupling of a charge control to a high-voltage capacitor and, additionally, controls the coupling of the high-voltage capacitor to the cuvette. A curren t diverter diverts current away from the sample whenever an arc condition commences or a low sample resistance is detected across the cuvette. The current diverter includes a current diverter switch, which is triggered when a sense resistor measures a second predetermined voltage indicative of an arc- over event (or a low sample resistance condition).

公开(公告)号：CA2375362A1

公开(公告)日：2000-12-21

申请号：CA2375362

申请日：2000-05-09

Applicant: BIO RAD LABORATORIES

Inventor： RAGSDALE CHARLES W ,  TAGLIAMONTE JOHN

IPC: C12M1/00 ,  C12M1/42 ,  C12N13/00 ,  A61N1/08 ,  A61N1/30 ,  C12N15/64 ,  G01N37/00

Abstract: An electroporation system and method for directing high-voltage currents to a suspension of cells contained in a cuvette. A high-voltage switch controls t he coupling of a charge control to a high-voltage capacitor and, additionally, controls the coupling of the high-voltage capacitor to the cuvette. A curren t diverter diverts current away from the sample whenever an arc condition commences or a low sample resistance is detected across the cuvette. The current diverter includes a current diverter switch, which is triggered when a sense resistor measures a second predetermined voltage indicative of an arc- over event (or a low sample resistance condition).

公开(公告)号：DE60030713T2

公开(公告)日：2007-09-20

申请号：DE60030713

申请日：2000-05-09

Applicant: BIO RAD LABORATORIES

Inventor： RAGSDALE W ,  TAGLIAMONTE JOHN

IPC: C12M1/00 ,  C12N15/64 ,  C12M1/42 ,  C12M3/00

公开(公告)号：AU4708500A

公开(公告)日：2001-01-02

申请号：AU4708500

申请日：2000-05-09

Applicant: BIO RAD LABORATORIES

Inventor： RAGSDALE CHARLES W ,  TAGLIAMONTE JOHN

IPC: C12M1/00 ,  C12M1/42 ,  C12N15/64

公开(公告)号：EP1190075A4

公开(公告)日：2003-04-16

申请号：EP00928922

申请日：2000-05-09

Applicant: BIO RAD LABORATORIES

Inventor： RAGSDALE CHARLES W ,  TAGLIAMONTE JOHN

IPC: C12M1/00 ,  C12M1/42 ,  C12N15/64 ,  C12M3/00

CPC classification number: C12M35/02

Abstract: An electroporation system and method for directing high-voltage currents to a suspension of cells contained in a cuvette. A high-voltage switch controls the coupling of a charge control to a high-voltage capacitor and, additionally, controls the coupling of the high-voltage capacitor to the cuvette. A current diverter diverts current away from the sample whenever an arc condition commences or a low sample resistance is detected across the cuvette. The current diverter includes a current diverter switch, which is triggered when a sense resistor measures a second predetermined voltage indicative of an arc-over event (or a low sample resistance condition).