公开(公告)号：WO2017062535A3

公开(公告)日：2017-05-26

申请号：PCT/US2016055618

申请日：2016-10-06

Applicant: BIO-RAD LABORATORIES INC

Inventor： DELANOY MICHELLE ,  FLANAGAN JOHN ,  ARJOMANDI AUDREY ,  KAUL RAVI ,  BINDER STEVEN R

IPC: C07K14/20 ,  C07K19/00 ,  G01N33/53 ,  G01N33/569

CPC classification number: G01N33/56911 ,  C07K14/20 ,  G01N2333/20

Abstract: The present invention relates to an immunoassay for the detection of Borrelia specific IgG, IgM and IgG/IgM antibodies in biological samples suspected of Lyme infection. The immunoassay can be performed via a standard immunoassay format or on an automated platform. In various embodiments, the immunoassay uses one or more Borrelia specific chimeric peptides VlsE-FlaB (designated pFlaB-mV), VlsE-ErpP (designated pErp59-mV), VlsE-P35 (designated pP35-mV) alone or in combination with one or more outer surface protein C (Osp C) types B or I, p58 and DbpA. Other aspects of the invention provide antigen/substrate combinations and compositions comprising combinations of the disclosed peptides and/or proteins for use in the immunoassays described herein.

Abstract translation: 本发明涉及用于检测疑似莱姆病感染的生物样品中疏螺旋体属特异性IgG，IgM和IgG / IgM抗体的免疫测定法。 免疫测定可以通过标准的免疫测定方式或在自动化平台上进行。 在各种实施方案中，免疫测定法单独或与一种或多种疏螺旋体特异性嵌合肽VlsE-FlaB（命名为pFlaB-mV），VlsE-ErpP（命名为pErp59-mV），VlsE-P35（命名为pP35-mV） 更多外表面蛋白C（Osp C）B或I型，p58和DbpA。 本发明的其它方面提供了包含用于本文所述的免疫测定中的公开的肽和/或蛋白质的组合的抗原/底物组合和组合物。

公开(公告)号：CA2706681C

公开(公告)日：2017-03-07

申请号：CA2706681

申请日：2002-05-28

Applicant: BIO-RAD LABORATORIES INC

Inventor： BINDER STEVEN R ,  GOODRICH JODI L ,  SAFARIAN ZARA

IPC: G01N33/53 ,  G01N33/573 ,  G01N15/10 ,  G01N33/48 ,  G01N33/96

Abstract: Analyses of serum samples for the presence and amount of either of the two subunits of human Factor XIII protein are used as a means of eliminating a significant source of error that arises in the testing of serum and plasma. For serum samples, a negative result of an analysis for the presence of subunit a is a means of verifying that a sample is indeed serum, while a negative or positive result for subunit a serves to distinguish serum (negative) from plasma (positive). A positive result for the presence of subunit b is a means of verifying that the sample is either serum or plasma and not any other biological fluid. A quantitative analysis of subunit b is a means of verifying that the sample is of the intended volume rather than having been reduced in volume due to improper sampling. A quantitative analysis of subunit b is also a means of verifying the dilution of a sample of either serum or plasma.