公开(公告)号：WO2007044727A2

公开(公告)日：2007-04-19

申请号：PCT/US2006039571

申请日：2006-10-06

Applicant: CALIFORNIA INST OF TECHN ,  DIRKS ROBERT ,  PIERCE NILES A

Inventor： DIRKS ROBERT ,  PIERCE NILES A

IPC: C12Q1/70 ,  C12P19/34 ,  C12Q1/68

CPC classification number: C12Q1/6813 ,  C12Q2525/301

Abstract: The present application relates to the use of hybridization chain reaction (HCR) to form double stranded RNA polymers in the presence of a target, such as a nucleic acid associated with a disease or disorder. The RNA polymers are preferably able to activate the RNA-dependent kinase PKR. Activation of PKR via RNA-HCR can be used to treat a wide variety of diseases and disorders by specifically targeting diseased cells.

Abstract translation: 本申请涉及杂交链反应（HCR）在靶（例如与疾病或病症相关的核酸）存在下形成双链RNA聚合物的用途。 RNA聚合物优选能够激活RNA依赖性激酶PKR。 通过RNA-HCR激活PKR可用于通过特异性靶向患病细胞来治疗多种疾病和病症。

公开(公告)号：WO2005098049A3

公开(公告)日：2006-05-11

申请号：PCT/US2005009471

申请日：2005-03-22

Applicant: CALIFORNIA INST OF TECHN ,  DIRKS ROBERT ,  PIERCE NILES A

Inventor： DIRKS ROBERT ,  PIERCE NILES A

IPC: C12Q1/68 ,  C07H21/04 ,  C12P19/34

CPC classification number: C12Q1/682 ,  C12Q1/6813 ,  C12Q1/6823 ,  C12Q1/6825 ,  Y10T436/143333 ,  C12Q2537/1373 ,  C12Q2525/301 ,  C12Q2525/161 ,  C12Q2565/131 ,  C12Q2563/107 ,  C12Q2565/543 ,  C12Q2537/161 ,  C12Q2537/1376

Abstract: The present invention relates to the use of nucleic acid probes to identify analytes in a sample. In the preferred embodiments, metastable nucleic acid monomers are provided that associate in the presence of an initiator nucleic acid. Upon exposure to the initiator, the monomers self-assemble in a hybridization chain reaction. The initiator nucleic acid may be, for example, a portion of an analyte to be detected or may be part of an initiation trigger such that it is made available in the presence of a target analyte.

Abstract translation: 本发明涉及使用核酸探针来鉴定样品中的分析物。 在优选的实施方案中，提供了在引发剂核酸存在下缔合的亚稳核酸单体。 暴露于引发剂时，单体在杂交链反应中自组装。 引发剂核酸可以是例如待检测的分析物的一部分，或者可以是起始触发的一部分，使得其在靶分析物的存在下可用。

公开(公告)号：EP1931806A4

公开(公告)日：2010-05-26

申请号：EP06836249

申请日：2006-10-06

Applicant: CALIFORNIA INST OF TECHN

Inventor： DIRKS ROBERT ,  PIERCE NILES A

IPC: A61K31/715

CPC classification number: C12Q1/6813 ,  C12Q2525/301

公开(公告)号：EP1730161A4

公开(公告)日：2008-05-21

申请号：EP05730392

申请日：2005-03-22

Applicant: CALIFORNIA INST OF TECHN

Inventor： DIRKS ROBERT ,  PIERCE NILES A

IPC: C07H21/04 ,  C12P19/34 ,  C12Q1/68

CPC classification number: C12Q1/682 ,  C12Q1/6813 ,  C12Q1/6823 ,  C12Q1/6825 ,  Y10T436/143333 ,  C12Q2537/1373 ,  C12Q2525/301 ,  C12Q2525/161 ,  C12Q2565/131 ,  C12Q2563/107 ,  C12Q2565/543 ,  C12Q2537/161 ,  C12Q2537/1376

Abstract: The present invention relates to the use of nucleic acid probes to identify analytes in a sample. In the preferred embodiments, metastable nucleic acid monomers are provided that associate in the presence of an initiator nucleic acid. Upon exposure to the initiator, the monomers self-assemble in a hybridization chain reaction. The initiator nucleic acid may be, for example, a portion of an analyte to be detected or may be part of an initiation trigger such that it is made available in the presence of a target analyte.

公开(公告)号：DE602005023426D1

公开(公告)日：2010-10-21

申请号：DE602005023426

申请日：2005-03-22

Applicant: CALIFORNIA INST OF TECHN

Inventor： DIRKS ROBERT ,  PIERCE NILES A

IPC: C07H21/04 ,  C12P19/34 ,  C12Q1/68

公开(公告)号：AT480550T

公开(公告)日：2010-09-15

申请号：AT05730392

申请日：2005-03-22

Applicant: CALIFORNIA INST OF TECHN

Inventor： DIRKS ROBERT ,  PIERCE NILES

IPC: C07H21/04 ,  C12P19/34 ,  C12Q1/68

Abstract: The present invention relates to the use of nucleic acid probes to identify analytes in a sample. In the preferred embodiments, metastable nucleic acid monomers are provided that associate in the presence of an initiator nucleic acid. Upon exposure to the initiator, the monomers self-assemble in a hybridization chain reaction. The initiator nucleic acid may be, for example, a portion of an analyte to be detected or may be part of an initiation trigger such that it is made available in the presence of a target analyte.

公开(公告)号：AT526975T

公开(公告)日：2011-10-15

申请号：AT06836249

申请日：2006-10-06

Applicant: CALIFORNIA INST OF TECHN

Inventor： DIRKS ROBERT ,  PIERCE NILES

IPC: A61K31/715

Abstract: The present application relates to the use of hybridization chain reaction (HCR) to form double stranded RNA polymers in the presence of a target, such as a nucleic acid associated with a disease or disorder. The RNA polymers are preferably able to activate the RNA-dependent kinase PKR. Activation of PKR via RNA-HCR can be used to treat a wide variety of diseases and disorders by specifically targeting diseased cells.