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    PKR ACTIVATION VIA HYBRIDIZATION CHAIN REACTION
    1.
    发明申请
    PKR ACTIVATION VIA HYBRIDIZATION CHAIN REACTION 审中-公开
    PKR激活通过混合链反应

    公开(公告)号：WO2007044727A2

    公开(公告)日：2007-04-19

    申请号：PCT/US2006039571

    申请日：2006-10-06

    Applicant: CALIFORNIA INST OF TECHN ,  DIRKS ROBERT ,  PIERCE NILES A

    Inventor： DIRKS ROBERT ,  PIERCE NILES A

    IPC: C12Q1/70 ,  C12P19/34 ,  C12Q1/68

    CPC classification number: C12Q1/6813 ,  C12Q2525/301

    Abstract: The present application relates to the use of hybridization chain reaction (HCR) to form double stranded RNA polymers in the presence of a target, such as a nucleic acid associated with a disease or disorder. The RNA polymers are preferably able to activate the RNA-dependent kinase PKR. Activation of PKR via RNA-HCR can be used to treat a wide variety of diseases and disorders by specifically targeting diseased cells.

    Abstract translation: 本申请涉及杂交链反应（HCR）在靶（例如与疾病或病症相关的核酸）存在下形成双链RNA聚合物的用途。 RNA聚合物优选能够激活RNA依赖性激酶PKR。 通过RNA-HCR激活PKR可用于通过特异性靶向患病细胞来治疗多种疾病和病症。

    SYSTEM AND METHOD FOR NUCLEIC ACID SEQUENCE DESIGN
    3.
    发明申请
    SYSTEM AND METHOD FOR NUCLEIC ACID SEQUENCE DESIGN 审中-公开
    核酸序列设计的系统与方法

    公开(公告)号：WO2011126996A2

    公开(公告)日：2011-10-13

    申请号：PCT/US2011031127

    申请日：2011-04-04

    Applicant: CALIFORNIA INST OF TECHN ,  PIERCE NILES A ,  WOLFE BRIAN R ,  ZADEH JOSEPH

    Inventor： PIERCE NILES A ,  WOLFE BRIAN R ,  ZADEH JOSEPH

    IPC: G06F17/10

    CPC classification number: G06F19/22 ,  G06F19/16 ,  G06F19/24

    Abstract: Described herein are systems and processes for designing the sequence of one or more interacting nucleic acid strands intended to adopt a target secondary structure at equilibrium. The target secondary structure is decomposed into a binary tree and candidate mutations are evaluated on leaf nodes of the tree. During a process of leaf optimization, defect-weighted mutation sampling is used to select each candidate, mutation position with a probability proportional to its contribution to an ensemble defect of the leaf. Subsequences of the tree are then merged, moving up the tree until a final nucleotide sequence of interest is determined that has the target secondary structure at equilibrium.

    Abstract translation: 本文描述了用于设计在平衡时采用目标二级结构的一个或多个相互作用的核酸链的序列的系统和方法。 目标二级结构被分解为二叉树，并在树的叶节点上评估候选突变。 在叶优化过程中，使用缺陷加权突变抽样来选择每个候选者，突变位置的概率与其对叶的整体缺陷的贡献成比例。 然后将树的子序列合并，向上移动树，直到确定目标二级结构处于平衡状态的最终核心序列。

    TRIGGERED RNAi
    4.
    发明申请
    TRIGGERED RNAi 审中-公开

    公开(公告)号：WO2008106658A3

    公开(公告)日：2008-11-27

    申请号：PCT/US2008055559

    申请日：2008-02-29

    Applicant: CALIFORNIA INST OF TECHN ,  PIERCE NILES A ,  YIN PENG

    Inventor： PIERCE NILES A ,  YIN PENG

    IPC: A61K48/00 ,  C07H21/02 ,  C07H21/04 ,  C12N15/11

    CPC classification number: A61K31/7105 ,  C12N15/111 ,  C12N2310/14 ,  C12N2310/16 ,  C12N2310/53 ,  C12N2320/50 ,  Y02P20/582

    Abstract: The present application relates to methods and compositions for triggering RNAi. Triggered RNAi is highly versatile because the silencing targets are independent of the detection targets. In some embodiments, a method of silencing a target gene is provided. The method comprises providing an initiator to a cell comprising a detection target and a silencing target gene, wherein the detection target is different from the silencing target gene; providing a first substrate monomer to the cell, wherein the first substrate monomer comprises a silencing target complement region that is substantially complementary to a portion of the silencing target gene, and an initiator complement region that is substantially complementary to a portion of the initiator; and providing a second substrate monomer to the cell, wherein the second substrate monomer comprises a silencing target region that is substantially complementary to the silencing target complement region; wherein binding of the detection target to the initiator initiates formation of an inactivator double-stranded RNA (inactivator dsRNA) which silences the silencing target gene.

    Abstract translation: 本申请涉及用于触发RNAi的方法和组合物。 触发RNAi具有很高的通用性，因为沉默目标独立于检测目标。 在一些实施方案中，提供了沉默靶基因的方法。 该方法包括向包含检测目标和沉默靶基因的细胞提供引发剂，其中检测靶与沉默靶基因不同; 向所述细胞提供第一底物单体，其中所述第一底物单体包含与所述沉默靶基因的一部分基本互补的沉默靶标补体区域和与所述引发剂的一部分基本互补的引发剂补充区域; 以及向所述细胞提供第二底物单体，其中所述第二底物单体包含与所述沉默靶标补体区域基本上互补的沉默靶标区域; 其中检测靶与启动子的结合启动沉默沉默靶基因的失活子双链RNA（失活子dsRNA）的形成。

    Fractional initiator hybridization chain reaction
    10.
    发明专利
    Fractional initiator hybridization chain reaction 未知

    公开(公告)号：AU2017291727B2

    公开(公告)日：2021-07-08

    申请号：AU2017291727

    申请日：2017-06-30

    Applicant: CALIFORNIA INST OF TECHN

    Inventor： PIERCE NILES A ,  CHOI HARRY M T

    IPC: C07H21/00 ,  C07H21/02 ,  C12Q1/68

    Abstract: The present disclosure relates to methods and compositions involving HCR reactions that involve initiators that are split into two or more parts. Effective HCR is dependent upon two or more of these split initiators being brought into proximity (e.g., via binding events mediated by a target) such that a full initiator is formed that is capable of triggering HCR signal amplification.

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