公开(公告)号：WO02072892A9

公开(公告)日：2004-05-27

申请号：PCT/US0208187

申请日：2002-03-12

Applicant: CALIFORNIA INST OF TECHN ,  QUAKE STEPHEN ,  BRASLAVSKY IDO ,  HEBERT BENEDICT ,  KARTALOV EMIL

Inventor： QUAKE STEPHEN ,  BRASLAVSKY IDO ,  HEBERT BENEDICT ,  KARTALOV EMIL

IPC: G01N21/64 ,  B01L3/00 ,  C12M1/00 ,  C12N15/09 ,  C12Q1/68 ,  G01N21/05 ,  G01N33/53 ,  G01N33/536 ,  G01N33/566 ,  G01N37/00 ,  C07H21/02 ,  C07H21/04

CPC classification number: C12P19/34 ,  C12Q1/6827 ,  C12Q1/6858 ,  C12Q1/6874 ,  C12Q2535/125 ,  C12Q2565/629 ,  C12Q2565/601 ,  C12Q2563/107

Abstract: The invention provides methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension. Some applications of the invention utilize total internal reflection fluorescence microscopy to image polynucleotide molecules at single molecule resolution.

Abstract translation: 本发明提供了通过异步碱基扩展分析多核苷酸序列的方法和装置。 本发明的一些应用利用全内反射荧光显微镜以单分子分辨率对多核苷酸分子进行成像。

公开(公告)号：WO2005054441A3

公开(公告)日：2006-11-09

申请号：PCT/US2004040147

申请日：2004-12-01

Applicant: CALIFORNIA INST OF TECHN ,  QUAKE STEPHEN R ,  KARTALOV EMIL

Inventor： QUAKE STEPHEN R ,  KARTALOV EMIL

IPC: C12Q1/68 ,  B01J19/00 ,  B01L3/00 ,  C12N20060101 ,  F16K7/04 ,  F16K31/145

CPC classification number: B01J19/0046 ,  B01J2219/00432 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00657 ,  B01J2219/00677 ,  B01J2219/00722 ,  B01L3/5025 ,  B01L3/502707 ,  B01L2200/12 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2400/0481 ,  B01L2400/0688

Abstract: A substrate is provided that facilitates immobilization of nucleic acid molecules, including DNA molecules and RNA molecules. Also provided is a device that includes the substrate, which, for example, can be a chip. In addition, methods of using the substrate are provided, including, for example, methods of sequencing a DNA molecule anchored to the substrate, and methods for conducting the process of sequencing using such devices.

Abstract translation: 提供了有助于核酸分子（包括DNA分子和RNA分子）固定化的底物。 还提供了包括例如可以是芯片的衬底的器件。 此外，提供了使用底物的方法，包括例如对锚定于底物的DNA分子进行测序的方法，以及使用这些装置进行测序的方法。

公开(公告)号：WO2005069980A3

公开(公告)日：2005-09-29

申请号：PCT/US2005000211

申请日：2005-01-05

Applicant: CALIFORNIA INST OF TECHN ,  BALAGADDE FREDERICK ,  HANSEN CARL L ,  KARTALOV EMIL ,  QUAKE STEPHEN R

Inventor： BALAGADDE FREDERICK ,  HANSEN CARL L ,  KARTALOV EMIL ,  QUAKE STEPHEN R

IPC: B01L3/00 ,  C12M1/00 ,  C12M1/113 ,  C12M1/18 ,  C12M3/00 ,  C12P19/04 ,  C12P19/34 ,  C12Q1/02 ,  C12Q1/68

CPC classification number: C12P19/04 ,  B01L3/5027 ,  B01L3/502738 ,  B01L2300/0861 ,  B01L2300/0877 ,  B01L2300/10 ,  B01L2300/163 ,  B01L2400/0481 ,  B01L2400/0655 ,  C12M23/16 ,  C12M23/34 ,  C12M39/00 ,  C12P1/00

Abstract: A chemostat (Figure 1) that includes a growth chamber having a plurality of compartments, where each of the compartments may be fluidly isolated from the rest of the growth chamber by one or more actuatable valves. The chemostat may also include a nutrient supply-line to supply growth medium to the growth chamber (s), and an output port to remove fluids from the growth chamber. Also, presented is a method to prevent biofilm formation in a growth chamber of the chemostat. The method may include the step of adding a lytic agent to an isolated portion of the growth chamber and re-uniting the isolated portion with the rest of the growth chamber.

Abstract translation: 一种恒温器（图1），其包括具有多个室的生长室，其中每个室可以通过一个或多个可致动阀与生长室的其余部分流体隔离。 恒化器还可以包括向生长室供应生长培养基的营养供应线以及从生长室去除流体的输出端口。 还提出了防止生长膜在恒化器生长室中形成的方法。 该方法可以包括将溶解剂添加到生长室的分离部分并将分离的部分与生长室的其余部分重新结合的步骤。

公开(公告)号：EP1368497A4

公开(公告)日：2007-08-15

申请号：EP02726651

申请日：2002-03-12

Applicant: CALIFORNIA INST OF TECHN

Inventor： QUAKE STEPHEN ,  BRASLAVSKY IDO ,  HEBERT BENEDICT ,  KARTALOV EMIL

IPC: G01N21/64 ,  B01L3/00 ,  C12M1/00 ,  C12N15/09 ,  C12Q1/68 ,  G01N21/05 ,  G01N33/53 ,  G01N33/536 ,  G01N33/566 ,  G01N37/00 ,  C07H21/02 ,  C07H21/04

CPC classification number: C12P19/34 ,  C12Q1/6827 ,  C12Q1/6858 ,  C12Q1/6874 ,  C12Q2535/125 ,  C12Q2565/629 ,  C12Q2565/601 ,  C12Q2563/107

Abstract: The invention provides methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension. Some applications of the invention utilize total internal reflection fluorescence microscopy to image polynucleotide molecules at single molecule resolution.

公开(公告)号：EP1743019A4

公开(公告)日：2009-09-23

申请号：EP05722383

申请日：2005-01-05

Applicant: CALIFORNIA INST OF TECHN

Inventor： BALAGADDE FREDERICK ,  HANSEN CARL L ,  KARTALOV EMIL ,  QUAKE STEPHEN R

IPC: C12M3/00 ,  B01J19/00 ,  B01L3/00 ,  C12M1/00 ,  C12M1/113 ,  C12M1/18 ,  C12P19/04 ,  C12P19/34 ,  C12Q1/02 ,  C12Q1/68

CPC classification number: C12P19/04 ,  B01L3/5027 ,  B01L3/502738 ,  B01L2300/0861 ,  B01L2300/0877 ,  B01L2300/10 ,  B01L2300/163 ,  B01L2400/0481 ,  B01L2400/0655 ,  C12M23/16 ,  C12M23/34 ,  C12M39/00 ,  C12P1/00

公开(公告)号：CA2440754A1

公开(公告)日：2002-09-19

申请号：CA2440754

申请日：2002-03-12

Applicant: CALIFORNIA INST OF TECHN

Inventor： QUAKE STEPHEN ,  BRASLAVSKY IDO ,  KARTALOV EMIL ,  HEBERT BENEDICT

IPC: G01N21/64 ,  B01L3/00 ,  C12M1/00 ,  C12N15/09 ,  C12Q1/68 ,  C12Q1/6827 ,  C12Q1/6858 ,  C12Q1/6874 ,  G01N21/05 ,  G01N33/53 ,  G01N33/536 ,  G01N33/566 ,  G01N37/00 ,  C07H21/02 ,  C07H21/04

Abstract: The invention provides methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension. Some applications of the invention utilize total internal reflection fluorescence microscopy to image polynucleotide molecules at single molecule resolution.