Abstract:
Methods and algorithms for a multiplexed single detection channel amplification process and quantification of generated amplicons is presented. Various mathematical approaches for quantifying and verifying the amplicons in a reaction are presented. Usage of such methods and approaches allow upgrading of existing single and multiple channel instruments for further multiplexing capabilities.
Abstract:
Methods and algorithms for a multiplexed single detection channel amplification process and quantification of generated amplicons is presented. Various mathematical approaches for quantifying and verifying the amplicons in a reaction are presented. Usage of such methods and approaches allow upgrading of existing single and multiple channel instruments for further multiplexing capabilities.
Abstract:
Structures and methods are described for optical detection of physical chemical and/or biological samples. An optical detection structure may include a LED source multiple filters and single or multiple sample areas. A detector may be used to record a fluorescence signal. The sample area may allow the introduction of removable cartridges.