公开(公告)号：EP1490677A4

公开(公告)日：2006-01-18

申请号：EP03743260

申请日：2003-02-27

Applicant: CALIFORNIA INST OF TECHN

Inventor： DATTA DEEPSHIKHA ,  WANG PIN ,  CARRICO ISAAC ,  MAYO STEPHEN L ,  TIRRELL DAVID

IPC: C07K1/00 ,  C12N5/06 ,  C12N9/00 ,  C12N9/22 ,  C12N15/00 ,  C12P21/02 ,  G01N33/48 ,  G01N33/50 ,  G06F19/16 ,  G06F19/00

CPC classification number: C12N9/93 ,  C07K1/00 ,  C07K2299/00 ,  G06F19/16

公开(公告)号：WO2007047668A3

公开(公告)日：2008-04-03

申请号：PCT/US2006040541

申请日：2006-10-16

Applicant: CALIFORNIA INST OF TECHN ,  DIETERICH DANIELA C ,  TIRRELL DAVID ,  LINK AARON J

Inventor： DIETERICH DANIELA C ,  TIRRELL DAVID ,  LINK AARON J

IPC: A61K47/48 ,  A61K49/00 ,  A61K49/08 ,  A61K51/04 ,  G01N33/68

CPC classification number: A61K51/0497 ,  A61K47/54 ,  A61K49/0002 ,  A61K49/0047 ,  A61K49/085 ,  A61K49/10

Abstract: The invention provides methods, reagents and systems to preferentially mark fast-proliferating cells/tissues (such as cancer), by incorporating non-natural amino acids into proteins, preferably in vivo, using the endogenous protein synthesis machinery of an organism. The incorporated non-natural amino acids contain reactive groups for further chemical reagents, which may serve as a "handle" to for a number of uses, such as imaging of cancer cells, targeting drugs to preferentially kill cancer cells, and proteomic analysis in the context of large scale or high throughput screening for candidate drug leads that affects the proliferation of a target cell, etc.

Abstract translation: 本发明提供了通过使用生物体的内源性蛋白质合成机制将非天然氨基酸掺入蛋白质，优选在体内，优先标记快速增殖细胞/组织（例如癌症）的方法，试剂和系统。 合并的非天然氨基酸含有用于其他化学试剂的反应性基团，其可用作许多用途的“处理”，例如癌细胞成像，靶向优先杀死癌细胞的药物和蛋白质组学分析 用于影响靶细胞增殖的候选药物导联的大规模或高通量筛选的上下文等。

公开(公告)号：WO03073238A2

公开(公告)日：2003-09-04

申请号：PCT/US0306083

申请日：2003-02-27

Applicant: CALIFORNIA INST OF TECHN ,  DATTA DEEPSHIKHA ,  WANG PIN ,  CARRICO ISAAC ,  MAYO STEPHEN L ,  TIRRELL DAVID

Inventor： DATTA DEEPSHIKHA ,  WANG PIN ,  CARRICO ISAAC ,  MAYO STEPHEN L ,  TIRRELL DAVID

IPC: C07K1/00 ,  C12N5/06 ,  C12N9/00 ,  C12N9/22 ,  C12N15/00 ,  C12P21/02 ,  G01N33/48 ,  G01N33/50 ,  G06F19/16 ,  G06F

CPC classification number: C12N9/93 ,  C07K1/00 ,  C07K2299/00 ,  G06F19/16

Abstract: The instant invention provides methods, reagents, and computational tools for designing non-natural substrate analogs for enzymes, especially for designing unnatural amino acid analogs for aminoacyl tRNA Synthetases (AARSs), such as the Phe tRNA Synthetase. The instant invention also provides methods to incorporate unnatural amino acid analogs, especially those with interesting functional groups, into protein products to generate proteins of modified or novel functions.

Abstract translation: 本发明提供了用于设计用于酶的非天然底物类似物的方法，试剂和计算工具，特别是用于设计用于氨基tRNA合成酶（AARS）的非天然氨基酸类似物，例如Phe tRNA合成酶。 本发明还提供了将非天然氨基酸类似物（特别是那些具有感兴趣的官能团的类似物）掺入蛋白质产物以产生修饰或新颖功能的蛋白质的方法。

公开(公告)号：AT534909T

公开(公告)日：2011-12-15

申请号：AT05855111

申请日：2005-12-21

Applicant: CALIFORNIA INST OF TECHN

Inventor： LINK AARON J ,  SCHUMAN ERIN ,  TIRRELL DAVID ,  DIETERICH DANIELA C

IPC: G01N33/68

Abstract: The invention provides methods, reagents and systems for incorporating non-natural amino acids into proteins, preferably in vivo, using endogenous protein synthesis machinery of an organism. The incorporated non-natural amino acids contain reactive groups for further chemical reagents, which may serve as a " handle" to enrich the proteins or fragments thereof in a number of uses, such as proteomic analysis, imaging of diseased tissue/cells etc.

公开(公告)号：AU2003248370A1

公开(公告)日：2003-09-09

申请号：AU2003248370

申请日：2003-02-27

Applicant: CALIFORNIA INST OF TECHN

Inventor： TIRRELL DAVID ,  DATTA DEEPSHIKHA ,  WANG PIN ,  CARRICO ISAAC ,  MAYO STEPHEN L

IPC: C07K1/00 ,  C12N5/06 ,  C12N9/00 ,  C12N9/22 ,  C12N15/00 ,  C12P21/02 ,  G01N33/48 ,  G01N33/50 ,  G06F19/16

Abstract: The instant invention provides methods, reagents, and computational tools for designing non-natural substrate analogs for enzymes, especially for designing unnatural amino acid analogs for aminoacyl tRNA Synthetases (AARSs), such as the Phe tRNA Synthetase. The instant invention also provides methods to incorporate unnatural amino acid analogs, especially those with interesting functional groups, into protein products to generate proteins of modified or novel functions.