中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

17 records (took 0.028 seconds)

    DIRECTED PROTEIN DOCKING ALGORITHM
    1.
    发明申请
    DIRECTED PROTEIN DOCKING ALGORITHM 审中-公开
    指导蛋白质锁定算法

    公开(公告)号：WO03087310A2

    公开(公告)日：2003-10-23

    申请号：PCT/US0310535

    申请日：2003-04-04

    Applicant: CALIFORNIA INST OF TECHN ,  LOVE JOHN J ,  MAYO STEPHEN L

    Inventor： LOVE JOHN J ,  MAYO STEPHEN L

    IPC: G01N33/68 ,  G06F19/16 ,  G06F19/18 ,  C12N

    CPC classification number: G06F19/16 ,  G01N33/6803 ,  G06F19/18

    Abstract: The instant invention provides methods and computational tools for designing interaction between molecules based on their three-dimensional atomic coordinates. In a preferred embodiment, the method can be used to design protein-­protein interactions based on their three-dimensional structure. In one embodiment, the method of the instant invention includes a first step of docking interacting molecules based on their surface geometric fit by quantitative correlation techniques, followed by a second step of optimizing the resulting interacting surface by altering interface side-chains, such that the interfacial side-chains are repacked in a manner analogous to the cores of well-folded proteins. The method can be used in numerous applications, including redesigning interaction interfaces between known protein-protein, protein-polynucleotide, protein-carbohydrates (such as polysaccharide), protein-lipid (or steroid), enzyme-inhibitor, or antibody-target epitope pairs, or rational design of more potent drug molecules.

    Abstract translation: 本发明提供了用于基于它们的三维原子坐标来设计分子之间的相互作用的方法和计算工具。 在优选的实施方案中，该方法可用于基于其三维结构设计蛋白质 - 蛋白质相互作用。 在一个实施方案中，本发明的方法包括通过定量相关技术基于其表面几何拟合对接相互作用分子的第一步骤，随后是通过改变界面侧链优化所得到的相互作用表面的第二步骤，使得 界面侧链以类似于良好折叠蛋白质核心的方式重新包装。 该方法可用于许多应用，包括重新设计已知蛋白质 - 蛋白质，蛋白质 - 多核苷酸，蛋白 - 碳水化合物（如多糖），蛋白质 - 脂质（或类固醇），酶抑制剂或抗体 - 靶标表位对之间的相互作用界面 ，或合理设计更有效的药物分子。

    GENE RECOMBINATION AND HYBRID PROTEIN DEVELOPMENT
    4.
    发明申请
    GENE RECOMBINATION AND HYBRID PROTEIN DEVELOPMENT 审中-公开
    基因重组和杂交蛋白质的开发

    公开(公告)号：WO0190346A3

    公开(公告)日：2002-10-10

    申请号：PCT/US0116831

    申请日：2001-05-23

    Applicant: CALIFORNIA INST OF TECHN ,  WANG ZHEN-GANG ,  VOIGT CHRISTOPHER A ,  MAYO STEPHEN L ,  ARNOLD FRANCES H

    Inventor： WANG ZHEN-GANG ,  VOIGT CHRISTOPHER A ,  MAYO STEPHEN L ,  ARNOLD FRANCES H

    IPC: C12N15/10 ,  G06F19/22 ,  G06F17/50 ,  G06F19/00

    CPC classification number: G06F19/14 ,  C12N15/1027 ,  G06F19/22

    Abstract: The invention relates to improved methods for directed evolution of polymers, including directed evolution of nucleic acids and proteins. Specifically, the methods of the invention include analytical methods for identifying "crossover locations" in a polymer. Crossovers at these locations are less likely to disrupt desirable properties of the protein, such as stability or functionality. The invention further provides improved methods for directed evolution wherein the polymer is selectively recombined at the identified "crossover locations". Crossover disruption profiles can be used to identify preferred crossover locations. Structural domains of a biopolymer can also be identified and analyzed, and domains can be organized into schema. Schema disruption profiles can be calculated, for example based on conformational energy or interatomic distances, and these can be used to identify preferred or candidate crossover locations. Computer systems for implementing analytical methods of the invention are also provided.

    Abstract translation: 本发明涉及聚合物定向进化的改进方法，包括核酸和蛋白质的定向进化。 具体地，本发明的方法包括用于识别聚合物中的“交叉位置”的分析方法。 这些位置处的交叉链不太可能破坏蛋白质的所需性质，例如稳定性或功能性。 本发明还提供了用于定向进化的改进方法，其中聚合物在所识别的“交叉位置”选择性重组。 交叉中断配置文件可用于标识首选交叉位置。 还可以鉴定和分析生物聚合物的结构域，并将结构域组织成模式。 可以例如基于构象能量或原子间距离来计算模式中断简档，并且这些可以用于识别优选或候选交叉位置。 还提供了用于实现本发明的分析方法的计算机系统。

    GENE RECOMBINATION AND HYBRID PROTEIN DEVELOPMENT
    6.
    发明专利
    GENE RECOMBINATION AND HYBRID PROTEIN DEVELOPMENT 未知

    公开(公告)号：CA2405520A1

    公开(公告)日：2001-11-29

    申请号：CA2405520

    申请日：2001-05-23

    Applicant: CALIFORNIA INST OF TECHN

    Inventor： ARNOLD FRANCES H ,  WANG ZHEN-GANG ,  VOIGT CHRISTOPHER A ,  MAYO STEPHEN L

    IPC: C12N15/10 ,  G06F19/22 ,  G06F19/00 ,  G06F17/50

    Abstract: The invention relates to improved methods for directed evolution of polymers , including directed evolution of nucleic acids and proteins. Specifically, th e methods of the invention include analytical methods for identifying "crossov er locations" in a polymer. Crossovers at these locations are less likely to disrupt desirable properties of the protein, such as stability or functionality. The invention further provides improved methods for directed evolution wherein the polymer is selectively recombined at the identified "crossover locations". Crossover disruption profiles can be used to identify preferred crossover locations. Structural domains of a biopolymer can also b e identified and analyzed, and domains can be organized into schema. Schema disruption profiles can be calculated, for example based on conformational energy or interatomic distances, and these can be used to identify preferred or candidate crossover locations. Computer systems for implementing analytic al methods of the invention are also provided.

    Gene recombination and hybrid protein development
    8.
    发明专利
    Gene recombination and hybrid protein development 未知

    公开(公告)号：AU6341101A

    公开(公告)日：2001-12-03

    申请号：AU6341101

    申请日：2001-05-23

    Applicant: CALIFORNIA INST OF TECHN

    Inventor： WANG ZHEN-GANG ,  VOIGT CHRISTOPHER A ,  MAYO STEPHEN L ,  ARNOLD FRANCES H

    IPC: C12N15/10 ,  G06F19/22 ,  G06F19/00

    Abstract: The invention relates to improved methods for directed evolution of polymers, including directed evolution of nucleic acids and proteins. Specifically, the methods of the invention include analytical methods for identifying "crossover locations" in a polymer. Crossovers at these locations are less likely to disrupt desirable properties of the protein, such as stability or functionality. The invention further provides improved methods for directed evolution wherein the polymer is selectively recombined at the identified "crossover locations". Crossover disruption profiles can be used to identify preferred crossover locations. Structural domains of a biopolymer can also be identified and analyzed, and domains can be organized into schema. Schema disruption profiles can be calculated, for example based on conformational energy or interatomic distances, and these can be used to identify preferred or candidate crossover locations. Computer systems for implementing analytical methods of the invention are also provided.

    Gene recombination and hybrid protein development
    10.
    发明专利
    Gene recombination and hybrid protein development 未知

    公开(公告)号：AU2002365143A8

    公开(公告)日：2003-07-15

    申请号：AU2002365143

    申请日：2002-10-25

    Applicant: CALIFORNIA INST OF TECHN

    Inventor： VOIGT CHRISTOPHER A ,  ARNOLD FRANCES H ,  WANG ZHEN-GANG ,  MAYO STEPHEN L

    IPC: C12N20060101 ,  C12N15/10 ,  G01N33/48 ,  G16B10/00

    Abstract: The invention relates to improved methods for directed evolution of polymers, including directed evolution of nucleic acids and proteins. Specifically, the methods of the invention include analytical methods for identifying "crossover locations" in a polymer. Crossovers at these locations are less likely to disrupt desirable properties of the protein, such as stability or functionality. The invention further provides improved methods for directed evolution wherein the polymer is selectively recombined at the identified "crossover locations". Crossover disruption profiles can be used to identify preferred crossover locations. Structural domains of a biopolymer can also be identified and analyzed, and domains can be organized into schema. Schema disruption profiles can be calculated, for example based on conformational energy or interatomic distances, and these can be used to identify preferred or candidate crossover locations. Computer systems for implementing analytical methods of the invention are also provided.

Patent Agency Ranking