公开(公告)号：EP2155770A4

公开(公告)日：2011-12-07

申请号：EP08755764

申请日：2008-05-16

Applicant: CALIFORNIA INST OF TECHN

Inventor： YIN PENG ,  PIERCE NILES A

IPC: C12N15/10 ,  C07H19/00 ,  C12N15/63 ,  C12Q1/68

CPC classification number: C12N15/63 ,  B82Y5/00 ,  C12N15/10 ,  C12N15/1031 ,  C12Q1/6844 ,  C12Q2525/301 ,  C12Q2525/161

公开(公告)号：WO2008106658A3

公开(公告)日：2008-11-27

申请号：PCT/US2008055559

申请日：2008-02-29

Applicant: CALIFORNIA INST OF TECHN ,  PIERCE NILES A ,  YIN PENG

Inventor： PIERCE NILES A ,  YIN PENG

IPC: A61K48/00 ,  C07H21/02 ,  C07H21/04 ,  C12N15/11

CPC classification number: A61K31/7105 ,  C12N15/111 ,  C12N2310/14 ,  C12N2310/16 ,  C12N2310/53 ,  C12N2320/50 ,  Y02P20/582

Abstract: The present application relates to methods and compositions for triggering RNAi. Triggered RNAi is highly versatile because the silencing targets are independent of the detection targets. In some embodiments, a method of silencing a target gene is provided. The method comprises providing an initiator to a cell comprising a detection target and a silencing target gene, wherein the detection target is different from the silencing target gene; providing a first substrate monomer to the cell, wherein the first substrate monomer comprises a silencing target complement region that is substantially complementary to a portion of the silencing target gene, and an initiator complement region that is substantially complementary to a portion of the initiator; and providing a second substrate monomer to the cell, wherein the second substrate monomer comprises a silencing target region that is substantially complementary to the silencing target complement region; wherein binding of the detection target to the initiator initiates formation of an inactivator double-stranded RNA (inactivator dsRNA) which silences the silencing target gene.

Abstract translation: 本申请涉及用于触发RNAi的方法和组合物。 触发RNAi具有很高的通用性，因为沉默目标独立于检测目标。 在一些实施方案中，提供了沉默靶基因的方法。 该方法包括向包含检测目标和沉默靶基因的细胞提供引发剂，其中检测靶与沉默靶基因不同; 向所述细胞提供第一底物单体，其中所述第一底物单体包含与所述沉默靶基因的一部分基本互补的沉默靶标补体区域和与所述引发剂的一部分基本互补的引发剂补充区域; 以及向所述细胞提供第二底物单体，其中所述第二底物单体包含与所述沉默靶标补体区域基本上互补的沉默靶标区域; 其中检测靶与启动子的结合启动沉默沉默靶基因的失活子双链RNA（失活子dsRNA）的形成。