公开(公告)号：US20190100747A1

公开(公告)日：2019-04-04

申请号：US16130810

申请日：2018-09-13

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Rustem ISMAGILOV ,  Erik Jue ,  Daan Witters

IPC: C12N15/10 ,  B01L3/00 ,  B01L7/00 ,  G01N21/64

Abstract: A sample preparation module accepts a sample including a target analyte. The sample preparation module processes the sample through several reaction chambers and a solid phase column. Different reagents are present in the reaction chambers. The eluted analyte is then transferred to the amplification module, where it is further processed and amplified for optical analysis.

公开(公告)号：US11149265B2

公开(公告)日：2021-10-19

申请号：US16130810

申请日：2018-09-13

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Rustem Ismagilov ,  Erik Jue ,  Daan Witters

IPC: B01L3/00 ,  B01L7/00 ,  G01N21/64 ,  C12N15/10 ,  C12Q1/6806

Abstract: A sample preparation module accepts a sample including a target analyte. The sample preparation module processes the sample through several reaction chambers and a solid phase column. Different reagents are present in the reaction chambers. The eluted analyte is then transferred to the amplification module, where it is further processed and amplified for optical analysis.

公开(公告)号：US20170299483A1

公开(公告)日：2017-10-19

申请号：US15485431

申请日：2017-04-12

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Rustem F. Ismagilov ,  Erik Jue ,  Nathan Schoepp

IPC: G01N1/38 ,  B33Y10/00 ,  C12Q1/68 ,  B33Y80/00 ,  B01L3/00

CPC classification number: G01N1/38 ,  B01F5/0619 ,  B01L3/502 ,  B01L2200/026 ,  B01L2200/0621 ,  B01L2200/12 ,  B01L2400/0478 ,  B01L2400/0633 ,  B29C64/00 ,  B33Y10/00 ,  B33Y80/00 ,  C12Q1/6806 ,  G01N2001/382

Abstract: Devices and methods for biological sample preparation are provided herein. Components of such devices include a plurality of plungers that can be actuated for metering and/or mixing one or more agents. Methods of manufacturing such devices using 3D printing are also provided.

公开(公告)号：US11866695B2

公开(公告)日：2024-01-09

申请号：US17131449

申请日：2020-12-22

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Rustem F. Ismagilov ,  Erik Jue ,  Daan Witters

IPC: C12N15/10 ,  C12Q1/6806

CPC classification number: C12N15/1006 ,  C12N15/1013 ,  C12Q1/6806

Abstract: Methods and systems and related compositions for separating through a solid matrix a mixture comprising a nucleic acid in a concentration of 1 μM or less, together with a target compound having a water solubility equal to or greater than 0.001 g per 100 mL, which can be used for managing fluid flow, biochemical reactions and purification of the nucleic acid or other target analytes. The method comprises contacting the solid matrix with a target compound removing agent having a water solubility equal to or less than 0.05 g per 100 mL at 25° C. at 1 atm pressure with water having a solubility in the removing agent of less than 10 g per 100 mL at 25° C. at 1 atm pressure, the contacting performed to remove the target compound from the solid matrix.

公开(公告)号：US11795448B2

公开(公告)日：2023-10-24

申请号：US17473832

申请日：2021-09-13

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Rustem Ismagilov ,  Erik Jue ,  Daan Witters

IPC: C12N15/10 ,  B01L3/00 ,  B01L7/00 ,  G01N21/64 ,  C12Q1/6806

CPC classification number: C12N15/1003 ,  B01L3/508 ,  B01L3/50851 ,  B01L3/502753 ,  B01L7/52 ,  C12N15/1006 ,  C12N15/1013 ,  C12Q1/6806 ,  G01N21/6428 ,  G01N21/6456 ,  B01L2200/0631 ,  B01L2200/0689 ,  B01L2300/04 ,  B01L2300/069 ,  B01L2300/0672 ,  B01L2300/18 ,  B01L2400/0605

Abstract: A sample preparation module accepts a sample including a target analyte. The sample preparation module processes the sample through several reaction chambers and a solid phase column. Different reagents are present in the reaction chambers. The eluted analyte is then transferred to the amplification module, where it is further processed and amplified for optical analysis.

公开(公告)号：US11753635B2

公开(公告)日：2023-09-12

申请号：US17511235

申请日：2021-10-26

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Rustem Ismagilov ,  Erik Jue ,  Daan Witters

IPC: C12N15/10 ,  B01L3/00 ,  C12Q1/6806 ,  B01L7/00 ,  G01N21/64

CPC classification number: C12N15/1003 ,  B01L3/508 ,  B01L3/50851 ,  B01L3/502753 ,  B01L7/52 ,  C12N15/1006 ,  C12N15/1013 ,  C12Q1/6806 ,  G01N21/6428 ,  G01N21/6456 ,  B01L2200/0631 ,  B01L2200/0689 ,  B01L2300/04 ,  B01L2300/069 ,  B01L2300/0672 ,  B01L2300/18 ,  B01L2400/0605

Abstract: Methods and systems and related compositions for separating through a solid matrix a mixture comprising a nucleic acid together with a target compound having a water solubility equal to or greater than 0.01 mg per 100 mL, which can be used for managing fluid flow, biochemical reactions and purification of the nucleic acid or other target analytes.

公开(公告)号：US11174477B2

公开(公告)日：2021-11-16

申请号：US16132235

申请日：2018-09-14

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Rustem Ismagilov ,  Erik Jue ,  Daan Witters

IPC: C12N15/10 ,  B01L3/00 ,  C12Q1/6806 ,  B01L7/00 ,  G01N21/64

Abstract: Methods and systems and related compositions for separating through a solid matrix a mixture comprising a nucleic acid together with a target compound having a water solubility equal to or greater than 0.01 mg per 100 mL, which can be used for managing fluid flow, biochemical reactions and purification of the nucleic acid or other target analytes.